代用

The expression of NOSⅢ mRNA was detected semiquantitatively by reverse transcription-polymerase chain reaction.③Immun ocytochemistry was used to detect the expression of NOSⅢ protein.

将原代培养的CAEC进行缺氧1 h，复氧2、6、12和24 h，用逆转录-聚合酶链式反应和免疫细胞化学方法检测NOSⅢmRN A和蛋白质表达的变化。

Methods:Rat hepatocytes were isolatcd by a single two step perfusion method,placed in 96 hole tissue plates and incubated for 1.5 hours,then exposed to different concentrations of HGF for 48 hours and the proliferation of rat hepatocytes was determined by MTT colorimetric assay.

利用胶原酶原位二步灌注法分离大鼠肝细胞，接种并预培养 1 。5 h后，用含不同浓度 HGF的培养基继续培养至 48h，MTT比色法检测肝细胞生长因子对原代培养大鼠肝细胞增殖的影响。

So one topic of this thesis is to further study Ishikawa and Mann iterationmethods for solutions to nonlinear equations involving single-valued m ?

长期以来，许多作者用Ishikawa和Mann迭代法去逼近非线性算子方程的解以及非线性算子的不动点。

In chapter 3, as one of the main parts of this thesis, by using the mixed monotone method, we discussed the existence and uniqueness of singular elliptic boundary value problems.

第三章我们将用混合单调迭代法来证明奇异椭圆微分方程边值问题解的存在唯一性。

A four-point algorithm is presented in this paper for finding the least-squares solution of R and T,which is based on the singular value decomposition of matrix,and the derivation of the algorithm is provided too.

本文提供一种基于矩阵单值分解求解R和T的最小二乘解的四点算法，并对算法进行了推导，该算法与迭代法相比，具有速度快、用点少的优点，尤其适用于实际应用的颅脑手术可视化系统。

Methods The mRNA and protein expression levels of NCAM were detected by reversed transcriptive polymerase chain reaction and Western blot after the rat hippocampal neurons were incubated with 20, 40 and 80μg/ml sodium fluoride for 24 hours in vitro.

原代培养海马细胞暴露于20、40、80μg/ml氟化钠24 h后，用RT-PCR和Western blot方法分别检测细胞内NCAM mRNA和NCAM各蛋白亚型的表达。

After the solution was kept in the dark for five minutes,chloroform was added into it to adsorb selenium formed by previous reaction,then iodine separated out was determined by sodium hyposulfite titration using starch as the indicator,at last the content of selenium dioxide in the sample was foun.

在溶有二氧化硒的0.4～0.5mol/L盐酸介质中，加入过量的碘化钾，于暗处放置5min后，加入三氧甲烷吸附反应生成的硒，以淀粉为指示剂，用硫代硫酸钠标准溶液滴定析出的碘，测得二氧化硒的含量。

With ammonium persulfate/sodium thiosulfate as Redox initiator system,a binder based on vinyl acetate/acrylate copolymer used for chopped strand mat was prepared at low temperature.

以过硫酸铵/硫代硫酸钠为氧化还原引发体系，在低温下研究制备出了短切毡用的醋酸乙烯酯与丙烯酸酯类共聚物粘结剂。

Toxicity characterisation procedures indicated that the primary toxicants in the effluent were volatile,and could be reduced by sodium thiosulfate. Toxicity based fractionation techniques were conducted,and the oxidation organic chloridates were found not to be the major toxicants.

结果表明，废水对大型蚤具有急性毒性，曝气可去除废水的部分毒性，硫代硫酸钠的投加可去除废水毒性，同时用DPD试剂盒比色法可测出游离态氯气和一价氧化态氯化物。

VB programmers using numerical solution of the Jacobian iterative formula.

详细说明：用VB程序编写的数值计算解雅克比迭代公式。

Lugalbanda was a god and shepherd king of Uruk where he was worshipped for over a thousand years.

Lugalbanda 是神和被崇拜了一千年多 Uruk古埃及喜克索王朝国王。

I am coming just now,' and went on perfuming himself with Hunut, then he came and sat.

我来只是现在，'歼灭战perfuming自己与胡努特，那麼，他来到和SAT 。