代用

Culture tender leaves in culture medium of MS+2,4-D1.5mg/L+30g/L suger+0.7% agar pH5.8 for 20 days in darkness at 25℃, and then subculture to induced Ⅱ-type calli. Use forceps cutting the tissue to nubble with 2mm2, and put the tissue into Agrodacterium tumefaciens LBA4404 liquid supplemented with AS 100mg/L,then, co-culture 3 days, resume 7 days in MS+2,4-D1.5mg/L+30g/L suger+500mg/L cef, take to MS+2,4-D1.5mg/L+30g/L suger+100mg/L cef+10.0mg/L kanamycin culture 20 days in darkness. After that to make it polarize in MS+30g/L suger+100mg/L cef+10.0mg/L km. The percentage of km resistant callus was reached max after infection for 45 min, the average is 29.66%. Simultaneity, tender leave callus are infected 5 min by A. tumefaciens liquid in different negative pressure, and kept on 15 min in Agrodacterium tumefaciens liquid without negative pressure. Then screen out resistant callus and obtain transgenic plants. When the negative pressure is -0.05MP the percentage of km resistant callus was reached max, the average rate is 37.5%.

将心叶接种于MS+2.4-D1.5mg/L+30g/L 蔗糖，琼脂0.7%，pH5.8 培养基中25℃暗培养20d 后继代一次，诱导产生Ⅱ型胚性愈伤组织，用镊子将其夹碎成2mm2大小的小块，置入添加100mg/L AS 的根癌农杆菌LBA4404 菌液中，侵染时间为45min，共培养3 天后，转入MS+2.4-D1.5mg/L+30g/L 蔗糖+500mg/L Cef 培养基中恢复7天，再转入MS+2.4-D1.5mg/L+30g/L 蔗糖+100mg/L Cef+10.0mg/L Km 中，遮光培养20d后，置入其MS+30g/L 蔗糖+100mg/L Cef+10.0mg/L Km 分化，卡那霉素抗性愈伤组织获得率在侵染45min 时达到最大值平均为29.66%；同时以甘蔗心叶愈伤组织材料，通过循环水式真空泵，产生负压，设定不同的负压值，在农杆菌菌液中感染5min 后，在负压条件下继续侵染15min 后，筛选出抗性愈伤组织并获得转化植株，其中在负压为-0.05 时转化率达到最大值，其卡那霉素抗性愈伤组织获得率平均为37.5%。

For the characteristic of ring permanent magnet torque motor used in direct drive numerical control round table suffering from the effect of load disturbances and parameter variations, the H∞ controller design methodology without the orthogonality assumptions is combined with m-K iteration in m synthesis to design a m-H∞ speed controller in this paper.

针对直接驱动数控转台用环形永磁力矩电机易受负载扰动及参数变化影响的特点，将去除正交性假设的H∞控制器设计方法与m综合中的m-K迭代法相结合，设计了一个m-H∞速度控制器。

The delegate model of media person has Zhao Yahui of People's Daily reporter, original ahead uses net inconvenience, we are rear the phone that edits to pass him without precedent ground is nuncupative, for him era keeps rich guest content.

媒体人的代表典型有人民日报记者赵亚辉，最初前方用网不便，我们后方编辑无先例地通过他的电话口述，为他代写博客内容。

West has pointed out that the newly-emerged tactics of cultural differences is characterized by the substitution of one-dimensionality and homogeneity with multi-dimensionality and multiplicity, the replacement of the abstract general and universal by the concrete exceptional and singular, and the realization of the historicization contextualization and multi-dimentionalization through the emphasis on the accidental temporary uncertain indeterminate transformative and changeable.

柯尔内·韦尔斯明确指出，"新的文化差异性策略的明显特征在于用多样性、多元性和异质去取代一元和同质。拒斥抽象、一般和普遍，而代之以具体、特殊和个别。通过强调偶然、暂时、无常、不确定性、转换和变化而实现历史化、情境化和多元化。"

We can use value iteration to obtain the optimal value function, from which the optimal policy can be derived efficiently.

用值迭代的方法可以求解MDP的最优值函数，然后从最优值函数得到最优策略。

Firstly,the mixed-integer bi-level programming is transformed into a single level continuous optimization problem by virtue of penalty function concept and optimal value function tool.

利用罚函数思想和最优值函数的概念将混合整数双层规划转化为连续变量的单层非线性规划，然后用事先确定步长的凸组合算法迭代求解此单层非线性规划，进而得到原双层规划的局部最优解

The geometric structure, frontier orbital coefficients and frontier electron densities of the important lubricating oil additive Zinc dialkydithophosphate are investigated by using ab initio method.

采用量子化学从头算法，全优化计算了二烷基二硫代磷酸锌化合物的平衡几何构型和成键特徵，用前线轨道能级和组成以及前线电子密度等参数，探讨了ZDDP的润滑机理。

By introducing reasonable fundamental assumptions and the Green strain in orthogonal curvilinear coordinates,geometric equations expressed by the Green strain tensor for solving thin shells with large deformation are derived in this paper.

将正交曲线坐标下的格林应变张量引入到薄壳大变形分析，通过建立恰当的基本假设，直接导出了用格林应变张量表示的壳体变形几何方程，将该方程代入到本构方程，由能量原理得到了小应变非线性变形平衡方程、内力方程和边界条件，在此基础上提出了大应变变形的简化分析方法。

Rats with CHF were divided randomly into two groups, one group was injected dexamethasone(1mg /kg) intramuscularly at the first and forth day respectively,the other group was injected equi-volume saline likewise, and observing the changes of symptoms of rats with CHF; measuring rats hemodynamics index including blood pressure, heart rate and left ventricular end-diastolic pressure 4 days later. Part 2: To measure every group rats myocardialα1、β1、β2、β3-AR density by immunohistochemical method ,and to understand the AR density changes .Taking the first part rats'myocardium tissues for testing myocardialα1、β1、β2、β3-AR density and 5 normal rats for normo-contrast group and taking gray scale of slice as AR density. Part 3: The study enrolled 35 patients with DCHF. Patients were included if they had orthopnea and refractory edema due to acute DCHF that was severe and had taken more 1 week hospitalization and intravenous therapy in addition to diuretics.

观察心衰大鼠的症状，4天后对两组大鼠进行血流动力学检测，检测指标包括血压、心率和左室舒张末压；第二部分：取第一部分实验动物大鼠的心肌组织，并取5只正常大鼠的心肌组织作为正常对照，用免疫组化法检测大鼠心衰前后及糖皮质激素治疗前后大鼠心肌组织肾上腺素能受体α1、β1、β2、β3受体的变化，取镜下切片的灰度值反应受体的密度；第三部分：研究对象为临床难治性心衰病人35例，病人的选择标准包括由于急性失代偿的心力衰竭出现的端坐呼吸，难治性水肿，患者病情严重，住院超过1周，包括利尿剂之外药物的静脉治疗。

The genetic relations of some subpopulations in the fourth generation of selection in outbreeding population of winter wheat were examined by RAPD techniques and quantitative analysis.

用RAPD分子标记技术及数量遗传分析手段对小麦异交群体趋异选择4代得到的各子群体的遗传关系进行了分析，并对两种结果进行了相关分析。

Lugalbanda was a god and shepherd king of Uruk where he was worshipped for over a thousand years.

Lugalbanda 是神和被崇拜了一千年多 Uruk古埃及喜克索王朝国王。

I am coming just now,' and went on perfuming himself with Hunut, then he came and sat.

我来只是现在，'歼灭战perfuming自己与胡努特，那麼，他来到和SAT 。