代用

Methods The lymphocytes of rats were isolated from the peripheral blood and cultured with sodium arsenite under 37 ℃.The ROS content was detected by DCFH-DA;The content of LPO was detected by fluoresce method.

体外分离大鼠外周血淋巴细胞后，施加处理因素，在37℃条件下恒温培养，用2'7'-二乙酰二氯荧光素染色法检测细胞内的ROS水平，用硫代巴比妥酸荧光法测定细胞内LPO含量。

Conceal disturbing elements like iron,copper,lead with hydrogen fluoride amine-EDTA,add the potassium iodide to separate iodine, and take starch as indicator,titrate with sodium hyposulfite.

试样以王水分解、泡塑富集分离金，硫脲解脱，以硝酸-硫酸破坏硫脲，用氟化氢胺-EDTA掩蔽铁、铜、铅等干扰元素，加碘化钾使之析出碘，以淀粉为指示剂，用硫代硫酸钠进行滴定。

In IPO-FDTD method, PO is used to calculate the scattering of the airfoil and FDTD is used to calculate the scattering of the suspender.

在该方法中，机翼的散射用PO求解，外挂物的散射用FDTD方法求解，通过通过多次迭代考虑两者之间的耦合效应。

The United States to achieve the target of three generations, we have introduced a generation to go beyond, way of life has achieved a "triple jump."

美国用三代人达到的指标，我们用一代人就实行了超越，生活方式也实现了"三级跳"。

With the aid of Zemax software, a three-mirror, unobscured, high-resolution optical system with large aperture, long focal length was successfully aligned by first collecting interferograms from several fields of all image plane, and obtaining the wavefront error, namely, obtained wavefront Zernike polynomials; then using the self-made program package to calculate the optical system misalignment.

利用Zemax光学设计软件与自编计算机辅助装调软件，实现了对大口径、长焦距、无中心遮拦离轴三反射镜光学系统的装调。通过Zemax软件模拟光学系统的失调模式，得到整个光学系统的波前像差，把波前像差代入到自编的复杂光学系统计算机辅助装调软件中，计算出光学系统的失调量，与引入的失调量对比，证明了其正确性。在实际的装调过程中，用小型球面干涉仪分别收集3个视场的干涉图，得到失调光学系统的失调量，用Zemax软件验证失调量数据的正确性，从而指导装调。

Adenosine was used as the raw material, dissolved in triethyl phosphate and then esterified with thiophosphoryl chloride under 0~5℃ for 8h without water and oxgen, in alkaline condition provided by 2,6-dimethylpyridine, introducing thiophosphoryl ester to adenosine.Then superfluous triethylamine was added and reacted under 5℃ for 1h to synthesize rough 5'-AMPS.The unpurified product was appled to a DEAE Sephadex A-25 column and eluted with triethylammonium bicarbonate.

用腺苷为起始原料，溶解于磷酸三乙脂中后，在无水无氧环境中及2，6-二甲基吡啶提供的碱性条件下与三氯硫磷以0~5℃下发生酯化反应8h，将硫代磷酸酯引入腺苷，然后再与过量三乙胺在5℃下反应1h，合成粗产品，再用碳酸氢三乙胺作洗脱液，过DEAE Sephadex A-25凝胶柱纯化。

METHODS: The BEC were preincubated with crocetin (1, 0.1 and 0.01 μmol/L) for 12 h, then exposed to AGE (100 mg/L). The RAGE mRNA expression was detected by RT-PCR analysis, the intercellular adhesion molecule-1(ICAM-1) was measured by ELISA. The extracellular superoxide ion and thiobarbituric acid reactive substances were assessed with superoxide ion kit and colorimetric assay, respectively The intracellular I2O2 was also detected using the probe 2,7-dichlorofluorescein. The mitochondrial membrane potential and mitochondrial Succinate dehydrogenase were analyzed by the retention of rhodamine 123 (Rh123) and MTT.

不同浓度的西红花酸(1、0.1、0.01μmol/L)预孵BEC细胞12h后，用AGE(100mg/L)刺激细胞12h，RT-PCR法测定RAGEmRNA的表达水平；ELISA法测定细胞间黏附分子－(ICAM-1)的表达；试剂盒分别检测胞外超氧阴离子和硫代巴比妥酸反应产物浓度；同时，还用2，7-二氯荧光素测定了胞内H2O2的浓度，并用罗丹明123(Rh123)荧光法及MTT法分别检测细胞线粒体膜电位水平和其琥珀酸脱氢酶的活性。

This method uses what Professor Salton has proposed as the vector spacial model to express text as an invisible vector in hyperspherical space.It makes use of certain pre-disposal methods (reduce the dimensions,clustering thesaurus,filtering out old-fashioned words) to reduce the dimensions.It usescosines to measure the degree of similaritybetween texts and uses the total similarity as the target convergence vector.It produces clustered results by using restricted iteratives to achieve partial perfection.

该算法用Salton教授提出的向量空间模型把文本表示成不可视的超球向量空间的一个向量，采用一定的预处理手段(降维、同义词归并、停用词过滤)完成维数约减，使用余弦相似度度量文本之间的相似度，并选择总凝聚度作为目标收敛函数，通过有限次的迭代达到局部最优，输出聚类结果。

The PEI grafted on the surface of nylon was modified by the alkyl halide such as bromohexane and iodomethane,to give high-molecular-weight antimicrobial quaternary ammonium salts.

尼龙6用4-溴丁酰氯活化，再与高分子聚乙烯亚胺在有机溶剂中进行反应，使PEI共价接枝在载体上，然后用卤代烷对接枝在尼龙6上的PEI进行烷基化反应，使其形成具有杀菌功能的高分子季铵盐，制备出尼龙树脂固定化的高分子抗菌杀生剂。

The nylon was activated with organic functional groups,and after that reacted with high-molecular-weight polyethyleniminein the organic soleent. PEI was grafted covalently on the surface of nylon. The PEI grafted on the surface of nylon was modified by the alkyl halide such as bromohexane and iodomethane,to give high-molecular-weight antimicrobial quaternary ammonium salts.

摘 要：尼龙6用4-溴丁酰氯活化，再与高分子聚乙烯亚胺在有机溶剂中进行反应，使PEI共价接枝在载体上，然后用卤代烷对接枝在尼龙6上的PEI进行烷基化反应，使其形成具有杀菌功能的高分子季铵盐，制备出尼龙树脂固定化的高分子抗菌杀生剂。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐