代用

The basic idea is to set a continuous solution of the region with a finite number of discrete points instead of a grid consisting of, these discrete points called grid nodes the solution of a continuous area on a given function of continuous variables used in the definition of discrete grid variable function approximation the original equation and boundary conditions in the micro-business operators to approximate differential, integral with the points and to approximate, so the original differential equations and boundary conditions are replaced by algebraic equations near Side, that is, finite difference equations , solve this equation group can get the original problem in discrete points on the approximate solution.

基本思想是把连续的定解区域用有限个离散点构成的网格来代替，这些离散点称作网格的节点；把连续定解区域上的连续变量的函数用在网格上定义的离散变量函数来近似；把原方程和定解条件中的微商用差商来近似，积分用积分和来近似，于是原微分方程和定解条件就近似地代之以代数方程组，即有限差分方程组，解此方程组就可以得到原问题在离散点上的近似解。

Parsley, sage, rosemary and thyme Remember me to one who lives there She once was a true love of mine Tell her to make me a cambric shirt (On the side of a hill, in the deep forest green) Parsley, sage, rosemary and thyme (Tracing of sparrow on snow-crested brown) Without no seams nor needlework (Blankets and bedclothes the child of the mountain) Then she'll be a true love of mine (Sleeps unaware of the clarion call) Tell her to find me an acre of land (On the side of a hill a sprinkling of leaves) Parsley, sage, rosemary and thyme (Washes the grave with silvery tears) Between the salt water and the sea strand (A soldier cleans and polishes a gun) Then she'll be a true love of mine (Sleeps unaware of the clarion call) Tell her to reap it in a sickle of leather (War bellows blazing in scarlet battalions) Parsley, sage, rosemary and thyme (Generals order their soldiers to kill) And to gather it all in a bunch of heather (And to fight for a cause they've long ago forgotten) Then she'll be a true love of mine

香菜、鼠尾草、迷迭香和百里香请代我向她问候她曾是我的挚爱请她为我做一件棉衬衫(在山丘的那一边，在蓊郁的森林里)香菜、鼠尾草、迷迭香和百里香不能有接缝，也不能用针线(毛毯、床单和山上的孩子)这样，她就可以成为我的挚爱(睡梦中浑然不觉那嘹亮的召唤)请她为我找一亩地(在山丘的那一边，几片树叶飘落下来)香菜、鼠尾草、迷迭香和百里香地必须位在海水和海岸之间(一位士兵洗净并擦亮了步枪)这样，她就可以成为我的挚爱(睡梦中却浑然不觉那嘹亮的召唤)请她用皮制的镰刀收割(战争在鲜红的军营里怒吼燃烧)香菜、鼠尾草、迷迭香和百里香(将军下令士兵们开始杀戮)用石南草捆扎成束为了一个他们早已遗忘的理由而战斗这样，她就可以成为我的挚爱 Hijo de la luna月亮之子 Tonto el que no entienda搞不懂的愚人哪 Cuenta una leyenda这是一个传说 Que una hembra gitana关于一名吉普赛女郎 Conjuró a la luna hasta el amanecer。

Data of tooth surface before and after wear were both obtained through laser scan system. The images were then reconstructed by SURFACER software.(2) C language program used of improved iterative closest point algorithms was developed to registrate imagines before and after wear. Standard plan was defined to the overlapped imagines after registration, then wear quantity was measured.￤ear was imitated by tooth brush machine which was made by STOMATOLOGY COLLEGE FORTH MILITARY MEDICAL UNIVERSITY.

应用三维激光扫描仪采集牙模型的表面数据，在计算机中用SURFACER逆向工程软件重建牙表面；②运用最近邻点迭代匹配算法加以改进，编制 C语言程序，配准磨耗前后的三维图像，在确定基准平面后，计算磨耗前后的体积变化量；③用口腔医院自制刷牙机进行模拟磨耗，用传统称重法，通过电子天平测试件的重量变化，用失水法计算测试材料的密度，换算成体积。

The authers fetched the embryo calvarial peristeum tissue, got human osteoblast by enzyme-assimilating methods and tissue-block culture methods. We observed the morphological change, growth feature and osteogentic capability, of osteoblast during culture in vitro with phase contrast invert microscope, drew the growth curre and identified the cells by alkaline phosphatase dye. At same time, the morphology and bioactivity of 3-5th-generation osbeoblast and anabiotic cells was studied comparatively. 2. titanium particles were examined by scanning electron and the size was determined by semi-automated image analysis. The 3-5 th gereration of human osteoblast were cultured in medium with different concentration of particulates titanium alloy (1mg/ml, 0.1mg/ml, 0.01mg/ml). Cell growth and proliferation was detected by MTT method after 2、4、6 days that particles were added into medium and ALP activity was measured by kit after 4、7、10 days respectively. 3. With above same methods,the 3-5th generation of human osteoblasts were cultured for 3、6、9days after different concentration of particulates titanium alloy (1mg/ml, 0.1mg/ml, 0.01mg/ml) were added into the medium and OPG gene expression was quantified by RT-PCR.

1、取人胚胎颅骨骨膜，采有用酶消化法和组织培养法获取成骨细胞体外培养并传代，观察细胞形态，生物特点及成骨特性，并绘制生长曲线同时碱性磷酸酶染色鉴定成骨细胞以及比较冻存前3-5 代与冻存后成骨细胞的特点。2、电镜下观察钛合金颗粒的形态并测量其粒径，将不同浓度的钛合金颗粒(1mg/ml，0.1mg/ml，0.01mg/ml)与成骨细胞共同培养，分别于第2、4、6 天用MTT 法测量细胞增殖情况及4、7、10天用试剂盒检测碱性磷酸酶活性。3、分别将不同浓度的钛合金颗粒(1mg/ml，0.1mg/ml，0.01mg/ml)与成骨细胞基因培养3、6、9 天用RT-PCR 方法半定量测定骨保护素基因mRNA 的表达。

Methods Density gradient centrifugalization combined with adherence method were used to segregate and cultivate rat MSCs. MSCs cultivated to the 3 rd passage were characterized using flow cytometry technique. TGF-β1 with 1, 2 and 5 μg/L were pretreated on MSCs for 8 h, then serum and oxygen deprivation were used to cause analogous ischemia of MSCs in vitro. AnnexinV/PI flow cytometry technique was used to evaluate apoptosis and survival of MSCs.

采用密度梯度离心结合贴壁法分离、培养大鼠MSCs，对培养第3代的MSCs进行鉴定；用1、2、5μg/L的TGF-β1分别预处理细胞8 h，然后用血清剥夺和低氧处理建立MSCs体外模拟缺血性损伤模型，通过流式细胞术评价MSCs的凋亡及存活情况；用Western blot方法检测2μg/L的TGF-β1对Akt和p-Akt表达的影响。

METHODS: Bone marrow was sterilely separated from human. After heparinization, human BMSCs were harvested using density gradient centrifugation and adherence method. At the fifth passage, BMSCs at 1×108/L were incubated in the 6-well plate and divided into 2 groups. BMSCs in the edaravone group were 50% confluent and incubated in L-DMEM containing basic fibroblast growth factor and fetal bovine serum for 24 hours. After washing in PBS, these BMSCs were incubated in serum-free L-DMEM containing 20 mg/L edaravone for 24 hours. BMSCs in the blank control group were incubated in L-DMEM, supplemented with 10% fetal bovine serum.

无菌抽取的骨髓经肝素化后，采用密度梯度离心法及贴壁筛选法分离获得人骨髓间充质干细胞，传至第5代按1× 108 L-1接种于6孔板内，设立2组，依达拉奉组细胞达50%融合时用含碱性成纤维生长因子、胎牛血清的L-DMEM预诱导24 h，PBS洗涤后再用20 mg/L依达拉奉无血清L-DMEM诱导24 h；空白对照组始终用含体积分数为10%胎牛血清的L-DMEM培养，不加任何预诱导剂和诱导剂。

Now, however, have emerged in China, contrary to the terms of eggs, which used to describe those who came to China the second-generation foreigners or even third-generation, these people have the appearance of a typical Westerners, but since a long time with their parents living in China, but they have China hearts, the official so-called white gold.

然而，现在，在中国却出现了相反的名词鸡蛋，它用来比喻那些来华外国人的第二代甚至第三代，这些人有着典型西方人的外貌，却因为长期随父母生活在中国，而有着一颗中国心，正式所谓的白皮黄心。

This dissertation gives the damping iteration method and geome-tric-mean tracing method, both based on inversion of observed ground-water level data, and also gives the proportional iteration (or ortho-gonalization) method based on inversion of hydrogeologic gradient data.

本文建立了用水位观测资料进行反演的阻尼迭代法和几何均值追踪法，利用水力梯度资料进行反演的比例迭代法。

The initial values of adjustment parameters are obtained by using iteration algorithm , the cutter profile is simulated with computer, and the profile error is corrected by iterative process, then the optimum values of adjustment parameters can be achieved.

研究了加工螺旋槽用盘形成形铣刀调整参数的计算机算法，利用迭代算法得出调整参数的初值，利用计算机模拟刀具廓形，对廓形误差进行迭代修正，从而可获得调整参数的最优值。

Objective To study the mechanism of the inbitory effect of matrine on central nervous. Methods In the present study, incubation of postnatal Wistar rat cortex neurons in culture with matrine at 0.5, 1.0, 2.0mmol/L was carried out to have knowledge of the effect of matrine on the expressions of GABAR.

目的 观察苦参碱对鼠原代培养皮质神经元γ-氨基丁酸受体蛋白表达的影响，探讨苦参碱抑制中枢的作用机制方法对Wistar新生大鼠原代培养皮质神经元分别给予剂量 0.5、1.0、2.0mmol/L培养基的苦参碱进行药物干预，4h后用细胞免疫组化方法测定GABAR的蛋白表达。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐