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In this study, the genomic structure and regulatory elements of ApoD from 10 representive species including protozoan, invertebrate, protochordate and vertebrate were analyzed and compared. The genomic structure of ApoD is less conserved in organisms from protostome to deuterostome invertebrates, while it is highly conserved among chordates including amphioxus and verebrates. All four conserved cysteine residues are present in amino acid sequence of deuterostome ApoDs, while there are only two cysteine residues in amino acid sequence of protostomes ApoD. Structure divergence between protostome and deuterostome ApoD proteins suggests their function difference. The majority of regulatory elements are present in nearly all organism ApoD genes ranging from unicellular protozoan to mammals, suggesting that ApoD plays a very fundamental role, and possesses a conserved regulatory mechanism. However, there also exist some specific regulatory elements, which are present only in certain species and may perform some special roles.ApoD mRNA expression in murine NIH/3T3 fibroblasts exposed to various stresses such as as hydrogen peroxide and UV light shows dose-dependent increase. And a fly homolog of ApoD, Glial Lazarillo, whose overexpression results in increased resistance to hyperoxia as well as a extension of lifespan under normoxia and resistance to starvation without altering lipid or protein content.

本文首先从生物信息学角度对分属于原生动物、无脊椎动物、头索动物和脊椎动物类群的10种动物ApoD的基因结构及调控区的调控元件进行分析及比较，发现：(1)ApoD基因外显子-内含子结构从原生动物草履虫到原口动物再到后口动物海胆的进化过程中不保守，但在分析的几种脊椎动物中相当保守；(2)文昌鱼ApoD基因扮演从无脊椎动物到脊椎动物承上启下的角色，可能代表了脊椎动物ApoD基因原型；(3)四个半胱氨酸保守位点在后口动物中都存在，而在原口动物中只存在两个，原口、后口动物ApoD蛋白一级结构上的差异反映蛋白功能上可能也存在一定差别；(4)调控区大多数主要调控元件为不同动物共有，说明ApoD主要功能及其表达调控在进化中相当保守；(5)ApoD基因个别调控元件是随着物种进化而出现并开始发挥相关作用，如SF-1；还有一些调控元件在进化过程中还没有发现其规律，这说明ApoD某些功能和基因表达调控模式可能因物种不同而存在一定的差异。

Transcriptional activity of ACL recombinants normalized by Renilla was significant difference with pGL3-Basic expect for construct -27 and -15 (P＜0.01). Construct -919 contains highest activity. 3 times reduction of transcriptional activity from-919 to -679bp indicated that negative regulation factors located probably in this region. The activity started on construct -73 suggested the basal promoter activity was located within the -73 to +77bp region; Transcriptional activity of IDHβrecombinants was not significantly different between the recombinant -58 and pGL3-Basic. The activity started on construct -82, decreasing with the length of the fragment up to -164 in despite of a bit of fluctuation, and kept increasing from construct -164 up to -279. Thus, the basal promoter activity was located within the -82 to +16bp region, whereas the upstream 197bp conferred maximal transcriptional activity.

采用5'端缺失策略，结合启动子预测结果，分别构建了8个ACL基因和19个IDH3β基因启动子区重组子，将其转染猪PK15细胞系，并利用荧光素酶双报告基因系统检测了它们的活性，结果表明：在所构建的猪ACL基因5侧翼序列的8个重组子中，除pGL-ACL27和pGL-ACL15外，其它重组子的荧光素酶活性与阴性对照均达到极显著水平(P＜0.01)，表明它们均具有启动子活性，pGL-ACL919活性最强，到pGL-ACL679活性下降了将近3倍，说明从-919bp到-679bp区域可能存在负的调控位点，从pGL-ACL621到pGL-ACL73活性有小幅度下降，到pGL-ACL27活性降到与阴性对照水平无显著差异，表明维持该启动子的基本活性区域位于-73bp到+77bp之间；IDH3β基因启动子活性开始于pGL-IB82，然后虽然有些波动，但是活性一直下降直到-164bp处，之后活性又开始升高，直到-279bp处活性达到最高，这些表明维持该启动子的基本活性区域位于-82bp到+16bp之间，从-82到-279之间的179bp区域具有最高的启动子活性。

The 8th century, the Sharada script evolved out of the Gupta script, and was mostly displaced in its turn by Devanagari from ca.

从ca。八世纪开始，Sharada手稿从Gupta发展起来，主要地从ca。

In an offical careen between pain and joy, SuShi have gone through all kinds of hardships and difficulties. From early smooth sailing to late overgrown with brambles; From fall of vigor and vitality in his youth to full of carefree and confidence in HangZhou provincial governor; From limitless patriotic emotion in MiZhou provincial governor to tranquil spirit world during the exile in HuangZhou、HuiZhou and DanZhou,under influence of above factors, his Ci written was obviously divided into three stages: first, major of early HangZhou Ci was composed of unworried languange; second, major of MiZhou Ci was composed of unrestrained languange; third, major of HuangZhou、HuiZhou and DanZhou Ci was composed of broad-minded languange. However, whether unworried, unrestrained or broad-minded, it underlyingly reflected SuShi's depression in life.

在宦海沉浮中，苏轼历经磨难，从刚开始的一帆风顺到后期的荆棘丛生，从少年时的万丈豪气到杭州刺使任上的明艳亮丽，从密州刺使任上的无限爱国热情到流放黄州、密州、儋州的逐渐平静的心灵世界，这些都影响到他的词作，使他的词作明显的分成了三个阶段：一是由早期杭州词为主体构成的明艳语，二是由密州词为主体构成的狂放语，三是由黄州词为主体构成的旷达语，但是，不管是明艳、狂放还是旷达，其中在深层次里都流淌着苏轼人生失意的苦泪。

Begin to do a website to arrive from 2002 about the same now fast 7 years, what the someone says marriage of husband and wife has 7 years is urticant, I am afraid of also is about the same, also arrived one each already was in website career definitely the accession of choose, the website also did more than 77879, stand to recreational website and forum from pornographic net, to the industry website that does nowadays now, did not calculate on successful, but accumulated experience thinks to still be held out oneself much, draw up group by group especially will share everybody, today from talk about a search to collect on the result practice that interview leaves him does it, Pick up a pen to write an article rarely, not laugh at

从2002年开始做网站到现在差不多快七年了，人家说夫妻结婚都有七年之痒，我怕也是差不多了，也到了一个自已在网站生涯中决择的时侯了，网站也做了七七八八几多个，从色情网站到娱乐网站与论坛，到现在如今做的行业网站，没算上成功，但积累的经验自认为还挺多，特分批写出来分享给大家，今天就从谈搜索收录上面谈下自己的心得练下手，很少拾笔写文章，莫见笑

My life and thoughts are always full of passion and contradiction. I jumped out from a remote village, and began to observe all kinds of cities with an insight of scanning, at the same time I was guided to predict and think my future. I also jumped out from the experiences and thoughs of England philosopher Rossell、Fance litterateur Bront、Taiwanee Liuyong and American Chinese Wangan and so on, and began to face and plan my life with an attitude of thinking deeply. I jumped out from a poor and near broken family too, and began to think of my expectation and responsibility to my yesterday and tomorrow families.

我的生活和思想总是充满激情和矛盾，我从一个偏远的乡村跳跃出来以审视的目光洞察着各式各样的城市，同时把我的眼光引领到对未来的憧憬和思考上来；我又从英国哲学家罗素、法国文学家普朗特、台湾作家刘墉、美籍华裔企业家王安等名人的思想和经历中跳跃出来以思考的姿态面对和规划自己的人生；我也从一个普通贫穷但有些破裂的家庭中跳跃出来开始寻思自己对家庭的期望和自己对家庭的责任。

We have all been victimized at some point because of our gender, race, age, socio-economic status, and religion or lack thereof. Our first cry is slapped out of us as we are violently wrenched from the relative safety of the crimson universe deep within our mother's bodies. Born in blood and battered into breathing, life begins with brutality and baptizes with violence.

由于咱们的性别，种族，年龄，社会经济地位和宗教信仰，或者对这些的缺乏，咱们从某些点上看都是被捐躯着的当咱们被激烈地从咱们母亲体内深处深红世界的安全中扭出，咱们的熬头声啼哭被从咱们体内拍打出来在鲜血中出生、被猛击以至能呼吸，生命就这样由残忍开始，受暴力洗礼

Women also face many social and emotional challenges, which can lead them to become a slave to the scale, avoid exercise for fear of bulking up and fall for spot reduction and quick fix solutions, all of which only compound the problem,婷美上海婷美实体专卖店,郑州婷美专卖店, These fears, misconceptions and bugaboos, which hold so many women hostage, could easily fill a book, but let's skip over all that and talk about solutions that DO work.

女性经常面对来自社会和感情方面的困扰从某种程度上说这使得她们更难以摆脱如同奴隶的命运般的困境她们畏惧体育锻炼她们担心这些体育活动会使她们的腿部变粗胸部变小因而她们倾向于寻找快速而容易的塑身手段然而这将带来更多的问题恐惧误解以及如同幽灵一般的错误认识就象挟持人质的案犯一般控制了女性的思想和行为这几乎可以写成一本完整的书籍让我们揭开它们的面具还原它们的真实面目下文将重点讨论解决方案以及从现在开始改变对就是从现在。

The Quodlibeta were miscellanies generally in the form of answers to questions which as soon as a teacher had attained a widespread renown, began to come to him, not only from the academic world in which he lived, but from all classes of persons and from every part of Christendom.

该quodlibeta被杂记一般在形式问题的答案，其中尽快作为一个教师已达到了一个广泛的知名度，开始来，他不仅从学术的世界中，他住，而是从所有类别的人士，并从每部分基督教。

The second chapter probes into the way and feature of spreading on Gesar" s music, and the development and the changes of the music spreading since the involving of modern intermedium, by introducing the psalmodies in different categories. The third chapter analyses the types of Gesar" s lyrics and the patterns they could change. The forth chapter illustrates the principles of Gesar" s creating and several majority tone patterns are showed here. In the fifth chapter, the tones of Gesar are classified based on three aspects, that is, the tone" s name, utilization and the localstyles. In the sixth chapter, the feature of Gesar s tones is analyzed from the aspect of the characteristics of melody, the rhythm and time, the tone patterns and the music structure and so on. The last chapter discusses the forms of music performance and the methods that the psalmodies take to sing.

本文第一章从史诗《格萨尔》说唱结合艺术形式的产生与发展开始，对它的这种艺术形式中的说与唱分别做了介绍；第二章通过对《格萨尔》音乐创作主体——艺人的分类介绍，探讨了史诗音乐的传播方式、传播特点以及在现代传播媒介的介入下它的传播方式的发展与变化；第三章是对唱词体裁的分析和它的变化类型；第四章论述了音乐唱腔的创作原则以及几种最主要的唱腔类型；第五章从曲调的名称、曲调的运用以及地域风格特点三方面对《格萨尔》唱腔进行了分类；第六章结合谱例从旋律特征、节奏节拍、调式以及音乐结构等方面分析了《格萨尔》音乐唱腔的特点；第七章是对《格萨尔》音乐表演形式及艺人演唱方法的探讨。

And discuss some key point of integrated management system design, include hazard identification and risk assessment and risk control in the occupational health and safety management, the measure of satisfaction target of customer, the flawlessness of law system, the integration of the integrated management system and enterprise standardization system, unify lead institution and work orgnization, integreted internal audit and integrated management review.

同时，论述了一体化管理体系构建中的几个主要问题，包括职业健康安全管理中的危险源辨识、风险评价和风险控制、顾客满意度的科学监测、法律、法规体系的完善、一体化管理体系与企业标准化体系的有机结合、统一的领导机构和工作机构、一体化的内部审核和管理评审。

Deepwater Taranaki is investigated for its petroleum potential, using all available seismic data tied to shallow-water wells.

运用与浅水井相关的现有全部震波探测数据考察了深海区塔拉纳基盆地的油藏前景。