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In the model of tumor cells metastases with cell line A673, 10〓 tumor cells can be detected after only one ampliation with outer primers, which means we can find 1 tumor cells in 10〓 normal PBMC, and enlarged again with inner primers, 10〓 tumor cells can be detected, which means we can find 1 tumor cell in 10〓 normal PBMC. In the simulative model of tumor cells metastases with RNA, 10〓 tumor cell can be detected that is to say we can find 1 tumor cell in about 10〓 normal PBMC. In all 15 samples of 11 patients, 2 patients'EWS/FLI1 expressions were negative, no evidence of metastases was found followed up by 8 months and 30 months respectively. Semi-quantitative study was used to detect the expression level of EWS/FLI1 mRNA in the other 9 positive patients, and metastases occurred in 6 high-level expression patients in 2-24 months after the operation, no evidence of metastases in the other 3 low-level expression patients up to now.

应用A673细胞系建立的肿瘤转移模型,单独应用外引物进行一次扩增,可以检测到10〓级肿瘤细胞,即可以从10〓个正常细胞中检测到1个肿瘤细胞;而在此基础上再次应用内引物扩增,则可以检测到10〓级肿瘤细胞,即可以从10〓个正常细胞中检测到1个肿瘤细胞;在用RNA模拟的肿瘤细胞转移模型中,两次扩增可以检测到10〓级肿瘤细胞,即可以从10〓个正常细胞中检测到1个肿瘤细胞。11例患者的15份标本中,2例没有检测到EWS/FLI1 mRNA的表达,分别随访8个月和30个月,没有发现临床转移的证据;对其余9例阳性表达的病例进行半定量的研究,6例EWS/FLI1 mRNA高表达的病例,分别在术后2-24个月发生转移,而3例EWS/FLI1 mRNA低表达的病例,则至今没有发现转移的证据。

One pace teachs me to make a station, arrive from the domain name space, from analytic to upload, arrive from HTML CMS, I what let do not have a technology also made a way station, the scene warbling of the oriole at that time is in eye, a website from begin conception to build SEO, true, very excited, I am very clear remember these courses, begin corn of bought a CN, with the space of a 100M, spent 76 yuan, 75 yuan space, 1 yuan rice

一步一步教我做站,从域名到空间,从解析到上传,从HTML到CMS,让没技术的我也做起了一个小站,当时的情景呖呖在目,一个网站从开始构思建设到SEO,真的,很兴奋,我很清楚的记得这些过程,开始买的一个CN玉米,和一个100M的空间,花了76元,75元的空间,1元的米

The French army went on melting away and disappearing in the same ratio from Moscow to Vyazma, from Vyazma to Smolensk, from Smolensk to the Berezina, from the Berezina to Vilna, apart from the greater or less degree of cold, the pursuit and barring of the way, and all other conditions taken separately.

从莫斯科到维亚济马,从维亚济马到斯摩棱斯克,从斯摩棱斯克到别列济纳,从别列济纳到维也纳,法军就是按照上述比例减员和毁灭的,法军的减员和毁灭与天气寒冷的程度、追击、道路阻障以及一切其他的条件无关。

music academics conduct and actions is an academics, it have the system of oneself, from music of many main factor of feeling carnal knowledge arrive reasonableness understanding;from most basically and at a time when"a clap" arrive various note at a time when;arrive to reply beat from the simple time;from basic dance the step arrive action of combination, from shallow go into deep situation step launch, let student practice in operation gradually acquire study plain knowledge and basic technical ability of the music foundation.

音乐学科作为一门学科,它有着自身的体系,从音乐的诸多要素的感性经验到理性认识;从最基本的时值"一拍"到各种音符的时值;从单拍子到复拍子;从基本舞步到动作的组合,由浅入深地步步展开,让学生实践操作中逐步获得学习浅显的音乐基础知识和基本技能。

Thereupon, I am a chinese one potential small citizen and student and totally green round and round member, my address some my submittals:Let you,I,he from publicizee to the survey from investigating to the literature transition to come to, come to from the literature transition to the catchpole academic chair, again from the catchpole academic chair to give relief to a poisonous member.

因此,我作为中国的一位小公民兼学生兼共青团团员,我提出一些我的建议:让你、我、他从宣传到调查,从调查到文艺换醒,从文艺换醒到法警讲座,再从法警讲座到解救毒员。

In the 20th century, the emphasis in mathematics has shifted from local to global considerations, from lower dimensions to higher dimensions, from commutativity to non-commutativity,and from linearity to non-linearity.

20世纪数学的重点从局部转移到整体,从低维转移到高维,从交换转移到非交换,以及从线性转移到非线性。

Under the guidance of the Marxist dialectical materialism and the historical materialism, the author of this dissertation makes a relatively systematic study of the noumenal problem of teaching-learning, making use of the achievements in other subjects and the relevant studies of this problem in didactics, following the principle that the object should be done from the phenomenon to the essence.

本文以马克思主义辩证唯物论和历史唯物论为根本指导思想,借鉴有关学科的研究成果和教学论学科对此问题的相关研究,遵循研究对象从简单到复杂、从具体到抽象、从低级到高级、从现象到本质的认识路线,对教学本体问题进行了比较系统的研究。

The results are as follows: 1 mean length and weight tend to increase while dominant length and weight groups are noted to be increasing from Oct. 2004 to Sep. 2007; 2 by using length-weight formula, calculated weight value at the same length is higher for track-data than that for background-data; 3 growth parameters of L∞, W∞ and K respectively increase from 140.5 mm, 36.5 g and 0.28 a-1 to 186.0 mm, 118.5 g and 0.36 a-1 after artificial reef deployment; 4 appreciable increases are noted in body length and weight growth rate after artificial reef deployment, and inflexion age of weight growth increases from 2.55 to 2.58 years after artificial reef deployment; 5 there is an increase in critical biomass age from 3.21 to 3.77 years after artificial reef deployment; 6 there is a decrease in total instantaneous population mortality from 1.065 to 0.75 per year after artificial reef deployment; and 7 the overall population biomass of small yellow croaker stands at 131.5 tons.

结果表明:投礁后小黄鱼的平均体长和体重均呈上升趋势,优势体长组和体重组均向高值移动;同等体长情况下由投礁后体长体重关系式估算的体重比用投礁前的体长体重关系式估算的值大;投礁后小黄鱼个体生长方程的参数 L∞、W∞、K 值分别从投礁前的140.5 mm、36.5 g和0.28 a-1增加到186.0 mm、118.5 g和0.36 a-1;投礁后体长与体重生长速度与投礁前相比有所增大,且体重生长的拐点年龄从投礁前2.55 a提高到2007年的2.58 a;小黄鱼种群生物量增长的临界年龄从投礁前的3.21 a提高到3.77 a;小黄鱼种群的总瞬时死亡率从投礁前的1.065 a-1减小到0.75 a-1;鱼礁区小黄鱼资源量约

Chapter TEN promulgates the epoch-making transformations in the mode of thinking fulfilledby Karl Marx's philosophy:from substantive thinking to relative thinking, from epistemologicalthinking to practical thinking,from predeterminative thinking to epigenesis thinking.

第十章考察马克思哲学在思维方式上所实现的划时代变革:从实体思维到关系思维,从知性思维到实践思维,从直观思维到变革思维,从预成论思维到生成论思维。

From the Internet to WWW, from the food cycle networks in the environment to the metabolism networks in the creatures' bodies,from the scientific research collaboration network to kinds of political networks,social networks and economic networks, from the large-scale network electronic network to the global traffic network, people live in a world full of varieties of complex networks, which makes the research of complex networks necessary.

从Internet到WWW,从生态环境中的食物链网到生物体中的新陈代谢网络,从科研合作网络到各种政治、经济、社会网络,从大型电力网络到全球交通网络,人们生活在一个充满着各种各样的复杂网络的世界中。这也使得研究复杂网络成为必要。

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We have no common name for a mime of Sophron or Xenarchus and a Socratic Conversation; and we should still be without one even if the imitation in the two instances were in trimeters or elegiacs or some other kind of verse--though it is the way with people to tack on 'poet' to the name of a metre, and talk of elegiac-poets and epic-poets, thinking that they call them poets not by reason of the imitative nature of their work, but indiscriminately by reason of the metre they write in.

索夫农 、森那库斯和苏格拉底式的对话采用的模仿没有一个公共的名称;三音步诗、挽歌体或其他类型的诗的模仿也没有——人们把&诗人&这一名词和格律名称结合到一起,称之为挽歌体诗人或者史诗诗人,他们被称为诗人,似乎只是因为遵守格律写作,而非他们作品的模仿本质。

The relationship between communicative competence and grammar teaching should be that of the ends and the means.

交际能力和语法的关系应该是目标与途径的关系。

This is not paper type of business,it's people business,with such huge money involved.

这不是纸上谈兵式的交易,这是人与人的业务,而且涉及金额巨大。