介体

Cellular transfection and localization observation were performed to eucaryotic cells under mediation with liposome.

脂质体介导真核细胞进行转染并细胞定位观察。

Eukaryon expression vectors containing humanbcl-2 and bax cDNA were respectively transfected into HCC-9204 cells,HCC cellsthat express Bax stably and highly were established,and an HCC cell strain thatexpress Bcl-2 at a 100% positive rate was obtained by cloning.

用脂质体介导的基因转染法分别将含有人bcl-2和bax cDNA的真核表达载体转染到HCC-9204细胞中，建立稳定高表达Bax的肝癌细胞，并用克隆化的方法建立100％稳定表达Bcl-2的肝癌细胞株。

For the purpose of constructing mammary gland specific expressional vector, we use the updream regulation sequence of bovine BLG to regulate lacS gene to express in mammary gland. Positive clone cells are selected and purified by the expression Neo gene and report gene EGFP. So the cells can be used as sources to create transgenic animals by nuclear transfer techniques.

本研究以牛β-乳球蛋白基因上游调控成分作为启动子，嗜热菌糖苷酶基因lacS为目的基因，构建了lacS基因乳腺特异表达载体，并通过脂质体介导法转染体外培养的牛胎儿成纤维细胞，借助新霉素抗性基因和增强型绿色荧光蛋白报告基因来筛选、纯化阳性克隆细胞，为利用核移植技术制备转基因动物提供细胞来源。

AIM:To investigate the effects of pEGFP-TGF-β1 plasmid DNA on proliferation and survival of human lens epithelial cell.

摘要目的：探讨脂质体介导的TGF-β1基因(pEGFP-TGF-β1)转染对人晶状体上皮细胞系B3(HLEC- B3)增殖、凋亡及细胞周期的影响。

Background: Chemokine receptors and their ligands play a key role in the recruitment of leucocytes and can mediate the recruitment of lymphocytes to normal small intestine.

背景：趋化因子受体及其配体是白细胞聚集的关键因素，可介导淋巴细胞聚集到正常的小肠。

Using plant bivalent expression vector pCAMBIA1300 and pBI121 as basic components, a new binary vector harboring tomato chitinase gene Chi3 and tomato β-1,3-glucanase gene Glu-Ac, was constructed through sequential restriction digests and ligations recombination. The two genes driven by CaMV35s promoter were successfully transferred into watermelon cultivars "ZhongYuYiHao" via Agrobacterium-mediated transformation.

构建了同时含有番茄儿丁质酶基因（Chi3）和β-1，3-葡聚糖酶基因的双价抗真菌基因植物表达载体，以西瓜子叶块为外植体，采用根癌农杆菌介导法，将Chi3和Glu-Ac同时导入西瓜栽培种&中育一号&，共获得46株抗性再生植株。

We firstly demonstrated that the effectiveness of gene transfer by SA liposomes in vivo.

我们首次证实SA脂质体介导的体内基因转染是有效的。

Autophagy is a process in which the subcellular membrane structures undergo dynamic morphological changes that lead to degradation of cellular proteins and organelles by lysosomes.

自噬是亚细胞膜结构发生动态变化并经溶酶体介导对细胞内蛋白质和细胞器降解的过程。

Utophagy is a process in which the subcellular membrane structures undergo dynamic morphological changes that lead to degradation of cellular proteins and organelles by lysosomes.

bstract 自噬是亚细胞膜结构发生动态变化并经溶酶体介导对细胞内蛋白质和细胞器降解的过程。

Results: it was efficient to transfect chicken embryonic fibroblast cells by the method of lipofectamine mediation. the expression levels of protein were significantly different.

结果 脂质体介导的方法能有效转染鸡胚胎成纤维细胞，实验中egfp蛋白相对表达量随质粒量的增加而明显升高（p.01）。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。