介体

Therefore,a sequence specific artificial zinc finger proteinis created when replacing the amino acids in the specific sites of C2H2 zinc finger protein and then fusing the protein to other activation or repression domains,while remaining the backbone of the zinc finger protein unchange...

ZFP可以介导靶基因的转录调控，抑制或激活特定基因的表达与配体依赖的靶基因激活或抑制；对DNA进行修饰，如人造限制性内切酶，重组酶，整合酶；抗病毒感染等。因此，人造锌指蛋白应用前景广阔，研究价值显著，是未来人类基因治疗的革命性的工具。

Objective Fluorescent-labeled antisense oligomer that is hybridized to 18-bp sequences next to the start coden of human proliferating cell nucleus antigen gene was used to assess the intracellular distribution and stability of the oligonucleotides in the presence or absence of liposome.

目的 观察硫代修饰型及天然型PCNA反义寡核苷酸在脂质体介导下或直接转染人膀胱癌细胞BIU-87后在细胞内的分布及其稳定性。

Objective To evaluate effect of hemagglutinating virus of Japan -liposome mediated tissue factor pathway inhibitor gene transfer on neointimal hyperplasia of endothelium denuded carotid artery of rat.

目的 探讨日本凝血病毒-脂质体介导的组织因子途径抑制剂基因转移对大鼠颈总动脉内皮剥脱术后新生内膜增生的抑制作用。

It was concluded that a heat-labile serum factor, most likely a type of complement-mediated IgM reaction, is responsible for inactivating phage in the blood.

我们认为，血清中存在的因子是导致噬菌体失活的主要因素，且很可能是由IgM介导的补体引发。

So in order to get recombinant NT-3, the rat NT-3cDNA was reconstructed in a mammalian expression vector and transfected into incasing CHO cells by liposome-mediated method. The clones of the cells transferred with rNT-3cDNA were selected by G418. The Chinese hamster ovary cell line stably producing recombinant rat NT-3 was established.

因此，为了获得基因重组NT-3，研究其生物效应，本文成功构建了重组大鼠NT-3基因真核生物细胞稳定表达载体，并利用脂质体介导，转染进CHO细胞；经G418筛选，建立了中国仓鼠卵巢细胞系，稳定表达重组大鼠NT-3。

METHODS Human IL 6 gene was reconstructed in retrovirus vector and transferred into incasing cells PA317 by lipofectamine mediated method. The clones of the cells transferred with hIL 6 were selected by G418. Targeted NIH3T3 cells were infected with the virus granules secreted from PA317 and also selected by G418. The insertion and expression of hIL 6 gene in NIH3T3 cells were analysed with Southern blot and Northern blot. RESULTS Human IL 6 retrovirus vector (pZIPIL-6) was successfully reconstructed.

利用重组载体构建技术将质粒pUCIL 6 cDNA的目的片段连接于逆转录病毒载体上，并以脂质体介导的方法将重组载体转染包装细胞PA317，以G418筛选克隆细胞，浓缩克隆细胞上清以制备重组病毒液，继之感染NIH3T3细胞后，进行Southern blot和Northern blot分析，检测目的基因在靶细胞的整合与转录水平。

The ability of erythrocytes to adhere tumor cell was completely interdicted by monoclonal antibody of CR1 or without complement.

红细胞的免疫粘附作用属补体依赖性CR1分子介导的天然免疫粘附。

Abstract］ Objective:To improve Lipofectin mediated transfection efficiency.

目的：提高脂质体介导的细胞转染效率。

HCRT potentiates NMDAR-mediated somatic release from LC neuronsApplication of 100μM NMDA evoked a pronounced secretion.This response to NMDA was subsequently enhanced by treatment with 100 nM HCRT for 5 min.

HCRT增强LC神经元NMDA受体介导的胞体递质分泌给予100μM NMDA可以显著引起分泌信号，随后用100 nM HCRT处理5 min，再次给予100μM NMDA可引起更大的分泌信号(增加大约40%)。

The conventional linear theories describe the linear part of the interaction between beam and wave very well.

等离于体平衡态分布与微波场有关，并且其介电特性随电场不是线性变化，因而是一非线性的栅。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。