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Studies of molecular genetics have demonstrated that retinoblastoma susceptibility gene is closely related to the development of retinoblastoma and the theory of Rb gene mutation or inactivation has been putatively proven to be the molecular mechanism of RB formation.

本研究以我国培养建立的一株RB细胞系〓及裸鼠为实验材料和对象,在对〓细胞抑癌基因(Rb基因、p53基因)表达状况进行检测和鉴定的基础上,通过建立裸鼠玻璃体腔RB常位异种移植模型,构建Rb基因的逆转录病毒表达载体,以脂质体介导的Rb基因转移方式,进行RB体内基因治疗的实验研究,观察Rb基因在体内对RB的治疗作用及影响因素,并探讨其作用机制。

All the transfected and un-transfected rMSCs were attached to Allo-DBMs. These new biomaterials were implanted in muscle bags and segmental radius defects of the New Zealand white rabbits, and some controlled material groups were established for comparison. All the biomaterials and the controlled materials were assessed by gross observation, radiographical and histological methods.

取兔自体骨髓基质细胞培养扩增,用脂质体介导的方法转染人骨发生蛋白2基因,将转染和未转染人骨发生蛋白2基因的自体骨髓基质细胞附和于异体兔脱钙骨基质形成新的生物植骨材料,连同空白及单纯脱钙骨基质对照组植入兔前肢肌袋和桡骨缺损。2、4、6、8周分别取材进行大体,放射线和病理观察。

PMW-PT, PNW-PT and PMW-PNN-PT ceramics with pure perovskite structure were prepared by the Semichemical Method or the Hydrothemal-Derived Precursor Method.

采用半化学法或水热前驱体法制备了纯钙钛矿结构的PMW-PT、PNW-PT和PMW-PNN-PT基弛豫铁电陶瓷粉体,探讨了钙钛矿相陶瓷粉体的形成机理,系统研究了各种影响因素对粉体或陶瓷的相组成、显微结构和介电性能的影响。

Identification of cripto-1 as a novel serologic marker for breast and colon cancer[J].

脂质体介导的cripto反义寡核苷酸抑制结肠癌细胞端粒酶活性[J]。

Results The morphology and asexual reproduction of Cloudina are broadly similar to modern serpulid annelids and its flourishing is somewhat coeval and corresponding to a positive excursion of δ13C.

结果 Cloudina可能是多毛类龙介虫,二者在管体形态特征、无性繁殖方式等方面存在可比性;Cloudina的出现都对应着碳同位素的明显正漂移,具有基本等时的特点。

Immunoelectron microscopic examination revealed that mGluR5 was prominently located in some neuronal somata and a lot of dendrites within the superficial layers of the spinal dorsal horn. Some dendrites containing mGluR5 constituted synaptic glomeruli with the terminals exhibiting the morphological characteristics of primary afferent C fiber. The ultrastructural features indicated that mGluR5 in the dorsal horn of the spinal cord may prominently work as a postsynaptical receptor to mediate or modulate the function of glutamate in the primary sensory transmission, including the nociceptive transmission.

免疫电镜研究显示,在脊髓背角浅层内,mGluR5主要定位在部分神经元胞体和大量树突内,并常见部分含mGluR5的树突与具有一级传入C纤维终末形态特征的轴突终末构成突触小球。mGluR5在脊髓背角浅层内的超微定位特征表明,脊髓背角内的mGluR5可能主要作为突触后受体介导或调节谷氨酸传递一级感觉的功能。

The method of which lipofectin mediated gene transfected cornea of rabbit by subconjunctival injection is practicable and secure.

脂质体介导基因经球结膜下注射转染兔眼角膜是可行而且是安全的。

Methods:Human CD80 cDNA was transfected into B16 melanoma cells by lipofectin-mediated gene transfer before the expansion of tumor cells were tested by MTT method in vitro;C57BL/6 mice were inculated subcutaneously either mock-transfected B16 cells (B16-neo)or CD80-transfected B16 cells (B16-CD80),then the latency,survival times and tumor mass growth were investigated.The lymphocytes were examined for both proliferation indices and specific cytotoxic activity by MTT method and improved LDH-releasing method,after syngenic Mixed Lymphocyte tumor cultures.

通过脂质体介导将CD80基因导入小鼠黑色素瘤B16细胞后,利用SABC法检测CD80的表达;MTT法测定肿瘤细胞体外增殖能力;将肿瘤细胞接种至同源小鼠皮下后观察成瘤期、荷瘤小鼠存活期及肿瘤结节生长速度;在同源淋巴细胞肿瘤细胞混合培养后,通过测定淋巴细胞增殖指数和CTL杀伤活性检测肿瘤细胞的免疫原性。

In the study, a set of near isogenic lines of CMS-S, S-Mo17Rf3Rf3 and S-Mo17rf3rf3, were adopted, and TdT-mediated dUTP nick-end labeling, in situ immunolocalization of cytochrome c and DNA ladder assay were employed to study the programmed cell death process on cytology and DNA level. The microscope observation evidenced that tapetal cells of fertile anthers kept intact until microspores, and then were disintegrated on later developmental stage.

S型细胞质雄性不育系的一对近等基因系S-Mo17Rf3Rf3 和S-Mo17rf3rf3为材料,采用TdT介导的dUTP DNA末端标记、细胞色素C免疫原位杂交和DNA寡聚核小体片段电泳等方法,分别在细胞学水平和DNA水平上研究了玉米CMS-S小孢子败育的细胞程序性死亡过程。

Using trimeric surfactant C_(14-2-14-2-14) as templating agent, the low cost and facile inorganic species sodium aluminate and sodium silicate as inorganic precursors, highly ordered 2D hexagonal aluminosilicate MCM-41 with high Al content were successfully synthesized.

利用廉价易得的硅酸钠和偏铝酸钠作为无机前驱体,以低聚季铵盐表面活性剂C_(14-2-14-2-14)为模板剂合成了高铝含量,高有序性的二维六方结构介孔硅铝酸盐材料,并对材料的结构、表面性质、形貌以及酸性进行了研究。

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On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面,更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值,分为几个相等的区间作为横轴,并将各区间内所测定值依所出现的次数累积而成的面积,用柱子排起来的图形,也叫做柱状图。

You rap, you know we are not so good at rapping, huh?

你唱吧,你也知道我们并不那么擅长说唱,对吧?