介体

The therapy groups displayed significant improvements in neuromotor functions and survivals of neuron and oligodendrocyte compared with control groups. These results demonstrated the neuroprotect roles of GGF2, and cationic liposome-mediated GGF2 transgene therapy has potential possibility.

结果：1、大鼠LFP致伤后3—7天，GGF2 mRNA在海马CAl区、CA2区、CA3区、DG及皮层表达明显增加。2、阳离子脂质体介导GGF2转基因治疗可促进大鼠伤后行为学的恢复，可促进损伤区神经元及少突胶质细胞的存活。3、原核表达方法得到GGF2重组蛋白。

Oxygen was found to depress their fcc-sctransition temperature Tc from the normal value of 260K,as indicated by theminimum in the temperature-dependent permittivity ε′,and also enlarge the drop in ε′at the transition.The magnitudes of the downward shift in Tc and the drop in ε′were roughly proportional to the O2 occupance at octahedral lattice sites,as estimatedfrom the resonance heights distribution.

发现氧的存在降低了以介电常数ε'温度谱极小值为标志的有序无序相变温Tc(正常相变温度为260K)，并且增大了ε'在相变前后的变化幅度，Tc的下降值和ε'的变化量Δε'近似比例于八面体位置氧的占据率，氧的占据率可以从核磁共振峰高的分布获得。

Some patients have ''monomicrobial bacterascites'' in which cultures are positive but there is normal ascitic neutrophil count.138 Such infections are thought to occur relatively commonly, and the majority are eradicated by the body's natural defence mechanisms (for example, opsonic and complement mediated bactericidal activity).138 139 When a positive culture is obtained, a further ascitic tap with a neutrophil count should be obtained.

有些患者有着&单一微生物细菌性腹水&，其腹水培养为阳性但腹水白细胞计数正常。138这些感染被认为较为常见，大部分可被机体天然的防御机制（如调理素和补体介导的杀菌活性）所根除。138，139当腹水培养为阳性时，应进一步进行腹穿并行嗜中性粒细胞计数。

This experiment is sought to study the contribution of different gene in osteogenous differentiation of bone marrow stromal cells and optimize the seed cells of bone tissue engineering.

为了优化骨组织工程种子细胞，探讨不同基因对骨髓基质干细胞成骨活性的影响，我们首先利用RT-PCR方法获得了全长BM P-2，VEGF 165及bFGF基因，克隆入真核表达载体pcDNA 3.0，鉴定正确后，经脂质体介导转入分离培养的大鼠骨髓基质干细胞，G 418筛选出稳定表达株。

The Mesozoic-Cenozoic in the Qaidam Basin contain abundant microfossils, especially in Ostracoda among which Cetacella、Cypridea、Austrocypris、Hemicyprinotus、Cyprideis and Ilyocypris inermis are index fossils for specific stratigraphical sequences since the Late Jurassic.

柴达木盆地中、新生界富含微体化石，节肢动物介形类分布尤其广泛，其中Cetacella、Cypridea、Austrocypris、Hemicyprinotus、Cyprideis和Ilyocypris inermis是晚侏罗世以来各相关层位的标准化石。

These results suggest that the activate spleen-energy method can achieve appetitive purpose by not only affecting the gastrointestinal tract function in periphery, but also regulating the process of ingestion in brain. The basic research about ingestion indicate: one hand, after food enters the gastro-intestinal tract, the endocrine cell of the intestinal tract is activated and secrete many kinds of brains intestines peptide, the level of the brain intestines peptide in blood as the periphery signal spreads to the central nervous system regions that control digest and feeding behavior. On the other hand, it has been shown that dorsal parabranchial neurons, containing CCK-8S, extend fibers to the VMH and are involved in the inhibition of feeding .

关于摄食控制的基础研究表明，一方面食物进入胃肠道后，激活肠道的内分泌细胞，分泌多种脑肠肽如CCK-8S，这些脑肠肽在血中的水平作为外周信号可通过&肠—脑轴&传入中枢神经系统介导消化和摄食行为的部位，影响摄食中枢神经元的活动调节动物的摄食行为；另一方面中枢神经元可合成释放内源性的神经肽直接作用于摄食中枢神经元调节摄食，如中枢鳃旁体神经元的神经纤维就可延伸到达VMH，并且合成释放内源性的CCK-8S。

The successful transfection of perifollicular cells was quantified by elisa.

制备得到的质粒脂质体制剂能够将质粒转染进皮肤细胞并介导体内瞬时表达。

Several wheat cultivars were transformed in planta by Agrobacterium tumefaciens and numbers of transformants were gained.

利用农杆菌介导的小麦活体转化技术转化多个小麦品种，得到一定数量的小麦转化体植株。

By using liposome-mediated gene transfection technology, plasmid pCMVSV40T/PUR containing SV40Tag was transfected in primary embryonic precartilaginous stem cells, while non-transfected cells served as negative controls.

利用脂质体介导基因转染技术将含有SV40Tag的质粒pCMVSV40T/PUR转染原代胚胎前软骨干细胞，以未转染细胞作阴性对照。

After having established genetic transformation system with tomato cotyledons as explant and determined the transformable of preculture time,incubation time and co-culture time,we set up the system of high frequency transformation of tomato cotyledons.Then hBMP-3m gene was transferred into tomato via Agrobacterium-mQdiated transformation,and the resistant plants to Hyg were obtained.

建立了以番茄子叶为外植体的遗传转化体系，确定了预培养时间、浸菌时间及共培养时间等转化条件，建立了番茄高频转化系统，然后通过农杆菌介导法将hBMP-3m基因导入番茄，并且获得了Hyg抗性植株。

Lugalbanda was a god and shepherd king of Uruk where he was worshipped for over a thousand years.

Lugalbanda 是神和被崇拜了一千年多 Uruk古埃及喜克索王朝国王。

I am coming just now,' and went on perfuming himself with Hunut, then he came and sat.

我来只是现在，'歼灭战perfuming自己与胡努特，那麼，他来到和SAT 。