介体

At present, regeneration system of cotyledons, hypocotyls, and stems, protoplasts and cotyledons codes have been established among Cucurbitaceae. There are several methods of transformation, such as agrobacteria -medidated genetic, particle gun, pollen tube passage, DNA soaking, ovary embryonary sac injection. Transgenic is tending to antivirotic and delayed maturity. Selection marker used often is NPT II.

目前，在葫芦科植物中已建立了子叶、茎尖、胚轴、茎段、原生质体和子叶节等的再生体系；用于遗传转化的方法有农杆菌介导法、基因枪法、花粉管通道法、DNA浸泡法等；用于转化的目的基因多为抗病毒病基因和延迟成熟的基因；NPTII基因为最常用的筛选标记基因。

The spheres are made up of dielectric material that has dielectric properties describable by the polariton and plasmon-type dispersion relationship.

计算了小球排列构成的体心立方结构的光子带隙性质，小球的介电函数可以是极化激元型或等离子型。

On the base of review the research development in tissue culture and genetic transformation, Comparative analyses were made on the callus induction and embryogenetic abilities of anther and non-pollinated ovules of rubber tree. Fluorescence detection was implemented with the different in vitro material of Hevea brasiliensis.

本研究在综述了橡胶树组织培养及遗传转化研究进展的基础上，系统地比较了橡胶花药和未授粉胚珠愈伤组织诱导及体细胞胚胎发生能力，对橡胶树不同离体组织进行荧光背景检测，将在植物胚胎发育时期起重要作用的BBM基因导入橡胶树，对电激法转化游离小孢子细胞以及农杆菌介导花药愈伤组织进行初步探索。

The ESCRT-III complex mediates membrane budding away from the cytosol in endosome biogenesis, cytokinesis, and viral budding.

ESCRT-III复合体能够在没有内涵体胞浆、胞浆移动和病毒芽殖的情况下，介导细胞膜的芽殖。

In this paper, an escherichia coli-saccharopolyspora erythraea shuttle vector containing the erya promoter region was constructed using the enhanced green fluorescent protein gene as a reporter. the shuttle plasmid was transformed into sac.erythraea a226 and streptomyces lividans jt46 by peg mediation, respectively. fluorescence microscopy confirmed that the egfp was expressed in both strains.

本文克隆了erya基因的启动子perya，以绿色荧光蛋白基因为报告基因，构建了大肠埃希菌-糖多孢红霉菌穿梭型质粒。peg介导原生质体转化法将穿梭型质粒分别转入糖多孢红霉菌a226与变铅青链霉菌jt46，荧光显微镜检测发现，此启动子在两菌株中都具有功能。

These transgenic cells were able to secrete hEGF protein to certain extent, and have the biological activities to enhance the growth rate of Hacat cells in vitro. Conclusion: Adult epidermal keratinocytes in vitro transfected with exogenetic hEGF cDNA can express and secrete active hEGF.

质粒pcDNA3.1-hEGF在脂质体介导下成功转染成人皮肤角质形成细胞，转基因细胞能分泌有生物活性的EGF；体外培养的成人皮肤角质形成细胞可见少量EGF分泌。

A modified Galanthus nivalis agglutinin gene was introduced into the genome of main cultivar 'Ningqi No.1' of Lycium bararum in vitro with explants of leaf disks mediated by Agrobacterium tumefaciens. Several factors affecting transformation efficiencies were studied based on induction rate of resistant callus. An effective system for genetic transformation of Lycium bararum was set up, and 70 kanamycinresistant transformants were obtained.

为了解决枸杞蚜虫侵害的难题，以宁夏枸杞主栽品种&宁杞1号&叶片为外植体，通过农杆菌介导的转化方法，将改造后的雪花莲凝集素GNA基因转入枸杞，以抗性愈伤组织诱导率作为转化效率的指标，对影响转化效率的因素进行了研究，初步建立了转化频率较高的转化系统。

Objective To study changes of dielectric properties of gastrocnemius caused by simulated weightlessness.

目的 在40 Hz～110 MHz频率范围观察模拟失重10 wk大鼠离体腓肠肌细胞介电特性的改变。

Objective To study changes of dielectric properties of gastrocnemius caused by simulated weightlessness.

目的在40Hz～110MHz频率范围观察模拟失重10wk大鼠离体腓肠肌细胞介电特性的改变。

MSCs were cultured in a conditional medium in subculture including: dexamethasone, vitamin C, and β-glycerophosphate. At the same time, we constructed the eukaryotic expression vector containing VEGF165gene. VEGF165 gene was transfected into MSCs by means of LipofectAMINE?2000. The stably gene expressive cells were screened with G-418 for 14 days.

在脂质体介导下将构建成功的真核表达载体pcDNA3.1- VEGF165转染到诱导培养的MSCs，G-418筛选阳性细胞克隆，将阳性克隆细胞和未转染的MSCs共同接种于β-磷酸三钙上，体外培养7天后，植入原大鼠肌肉内。

Lugalbanda was a god and shepherd king of Uruk where he was worshipped for over a thousand years.

Lugalbanda 是神和被崇拜了一千年多 Uruk古埃及喜克索王朝国王。

I am coming just now,' and went on perfuming himself with Hunut, then he came and sat.

我来只是现在，'歼灭战perfuming自己与胡努特，那麼，他来到和SAT 。