仅有的

ABSTRACT It is because that the excellent acceptors for maize transgenic engineering are insufficient in our country, especially in the southwest mountain areas of china and hereditary variation regularity for the two characters such as efficiency of embryonic callus induction and number of regenerating plant (these two characters were abbreviated to the nduction efficiency and number of regenerating in the following of the paper, respectively), which hint the maize culturing capacity, is not very clear. Therefore, aiming at picking out superior acceptors, we had made systematic researches on the two characters with combing traditional quantitative-character genetic analyzing methods such as single-factor genetic mating design, diallel crossing genetic design, genetic effect analyzing method and the modern molecular locating method such as QTLs'. The main results are followed.(1) 50 superior inbred lines and about 30 crosses in our country, especially in the southwest of China were used for identifying and selecting the superior genotypes in the above two investigated characters under the same culturing condition in 2000 and 2001. There was very significant difference among the genotypes in the both characters. But the two characters were not certainly related. Some genotypes such as 18-599 and 18-599 were very good in them. For some ones such as zong 31, induction was higher than 18-599 and 18-599 in the efficiency, but it was only 1/3 to the later in regenerating number. In some genotypes such as S37, R08, R15, P138, A318, induction efficiency was just about 3% and scarcely any regenerating plants were got. On the whole, hybrids acted better than inbreeds in the both characters.(2) Two kind of inbreeds were selected as parents of the Griffing's method 1. 18-599 and 18-599 and the inbred line zong 31 are one kind because they are not only superior in the characters of maize cross breeding, such as CA, resistance to disease and the important agricultural characters, but also excellent in transformation characters as the induction and regeneration.

针对我国、特别是西南山地所需玉米转基因工程育种优良受体极为匮乏和反应玉米幼胚培养能力的2个主要性状，即玉米幼胚胚性愈伤组织诱导率和胚性愈伤组织绿苗发生数的遗传变异规律十分不清楚的实际情况，本研究从筛选玉米转基因工程所需要的优良受体入手，采用单因素遗传交配设计、双列杂交遗传交配设计、世代基因效应等传统数量性状分析方法，以及现代分子标记定位主效QTL分析方法，对玉米幼胚胚性愈伤组织诱导率和胚性愈伤组织绿苗发生数等2个性状进行了较为系统的分析研究，取得以下主要研究结果：(1)于2000年和2001年通过对我国、特别是西南地区近50份优良自交系和近30个杂交组合，在相同培养条件下，对幼胚培养胚性愈伤组织诱导率和胚性愈伤组织绿苗发生数等2个幼胚培养能力性状进行了筛选与鉴定，发现玉米不同基因型具有完全不同的幼胚培养胚性愈伤组织诱导率和胚性愈伤组织绿苗发生数，但幼胚培养胚性愈伤组织诱导率与胚性愈伤组织绿苗发生数并不具有必然的相关关系，有的基因型，如自交系18-599和18-599在胚性愈伤组织诱导率和愈伤组织绿苗发生数等2个性状都表现相当优异；有的基因型，如自交系综31，仅幼胚培养胚性愈伤组织诱导率性状表现高于19-599和18-599，但在胚性愈伤组织绿苗发生数这一性状则与它们有相当大的差距，仅为19-599和18-599的1/3左右；有的基因型，如S37、R08、R15、P138、A318等玉米自交系不仅幼胚培养胚性愈伤组织诱导率很低，平均仅在3％左右，而且胚性愈伤组织绿苗发生数表现也很差，基本上没有分化成苗。

Despite doing a fine trade in his painting s, however, Canaletto died owning little more than his clothes, a few bits of furniture and some unsold pictures, which were left to his only relatives, two sisters and a nephew.

不过，尽管卡纳莱托卖画的生意很好，但他死时留给他仅有的亲戚一两个姐妹和一个侄子一的遗产，只有他的衣服、几件家具和几幅尚未出售的画。

This kind of abnormal psychology is also resulted from abnormal eiements of the past times.

五千年，这足以让动物都能听懂得语言。然而，在那仅有的光芒中，莱奥那多。

Every ton of gasoline and ammunition had to be brought in over the beaches in Normandy or through the single port of Cherbourg and transported by truck three to four hundred miles to the advancing front.

一吨吨的汽油、军火，都得从诺曼底海滩或仅有的港口瑟堡运进来，然后再用汽车运三、四百英里，才能送到不断向前推进的前线。

The shop assistant curtly informed me that there was only one type of pasty—traditional Cornish—but further probing did lead to a slightly embarrassed admission that a veggie option was available.

店员简短地告知我说，传统&康威尔&馅饼是店里仅有的馅饼品种，但经我百般询问，店员终于有些尴尬地告诉我们还有素馅馅饼可供选择。

C. Pont du Gard: it is an exceptionally well-preserved aqueduct that spans a wide valley in southern France.

加尔河上的引水渠：是绝无仅有的，目前保护最好的引水渠，它横跨法国南部河谷。

Two tenders for the construction of the Shillong Kun Shan Road and Bridge Group chairman, said: This is his 15-year history of unique road, and he had to worry about, shaken off, but eventually were infected with the ordinary people's desire to make up its mind in the determination of road .

负责二标段施工的山西隆坤路桥集团董事长说：这是他15年修路历史上绝无仅有的，他发愁过，动摇过，但最终还是被老百姓的愿望所感染下定了修路的决心。

A proper debate against a credible rival would allow the French to decide if Mr Sarkozy, once a shoo-in for re-election, should instead be only the second president in the Fifth Republic to be voted out after a single term.

而他与一位势均力敌的对手适时的唇枪舌战将使法国选民决定是否把这位曾经十拿九稳的连任者投票出局。如果真是这样，那么他将成为第五共和国历史上仅有的第二位只做满一个任期的总统。

Humber is one of only two schools with a literary agency to champion the publication of promising works.

亨伯是仅有的两所学校之一，文学机构，冠军的希望的作品的出版。

I only have my passport as I.D.

我仅有的身份证明是我的护照。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。