人工培养的

Various celeriac explants were in vitro cultured on MSO media added different ingredients in order to induce high quality somatic embryos suitable for making artificial seeds.

对根芹的不同外植体在附加不同成分的MS培养基上进行离体培养，以诱导适合于制作人工种子用的高质量体细胞胚。

The haploid breeding of maize is a technology that using anther culture or gynogenesis to produce haploid, and then obtain stable new varieties through natural or artificial doubling.

玉米的单倍体育种，是利用花药培养或孤雌生殖产生单倍体后，进行人工或自然加倍，迅速获得稳定的新品种的育种方法。

Paramoecium caudatum is one of the experimental materials used for teaching zoology. It is cultivated by artificial methods to improve the purity making it beneficial to teaching and research.

草履虫Paramoecium caudatum是动物学教学最常用的实验材料之一，一般采用人工方法培养提高草履虫的纯度，以便于应用于教学和研究。

Because of their difficult cultivation, cell culture is one of the most important ways of producing crocin family medicine.

为克服人工栽培藏红花的困难，采取细胞培养生产藏红花素类药物是重要的生产途径之一。

Used the dual culture, germination of spores and inoculation experiment , the strains of penicillium sp. had the strongest antifungal effectiveness to cytospora sp .of populus , so the strains of penicillium sp. could continue research .

综合两点对峙培养、孢子萌发和人工接种试验的结果可以看到青霉属菌株对杨树烂皮病菌的拮抗效果最好，因此，可对青霉属进行进一步试验为开发新的生防拮抗制剂打下基础。

The artificial cultivated one and "Tiepi Fengdou" are similar in the content of ten trace elements, while the content of nine trace elements in protocorm culture was higher than others, and the trace elements in "Tiepi Shihu Capsule" were relatively low.

微量元素含量的比较表明，人工栽培铁皮石斛中10种微量元素含量与铁皮枫斗大致相近，铁皮石斛组织培养物中除Cu之外的其他9种微量元素高于其他石斛材料，而铁皮石斛胶囊中微量元素含量相对较低。

SEM observation on the formation of asexual spores in Ustilaginoidea virens in potato sucrose agar medium was carried out.

对马铃薯蔗糖人工培养基上绿核菌不同培养时期的产孢情况进行了系统的扫描电镜观察。

The immunological traits such as titer,affinity,sensitivity and specificity of this mAb were characterized.The results showed that the conjugation ratio of SM to BSA in SM artificial antigen was about 25∶1.The titer of supernatant hybridoma cell lines of 3F9-C4 was 1∶3.2×10~2 by indirect ELISA.The isotypes in ascites was IgG_(2a)/κ,the affinity constant was 8.4×10~(11)L/mol,IC50 was 8.99μg/L,cross-reactivity to dihydrostreptomycin was 109.6% and little cross-reactivity to other compounds.

结果表明，BSA-SM人工抗原分子结合比为1∶25；筛选出3F9-C4敏感特异的杂交瘤细胞1株，间接ELISA测定细胞培养上清，效价为1∶3.2×102，同种型为IgG2a/κ；腹水的亲和常数为8.4×1011L/mol；IC50为8.99μg/L，与双氢链霉素交叉反应为109.6%，与其他SM结构相似物和功能近似物无交叉反应性。

The immunological traits such as titer, affinity, sensitivity and specificity of this mAb were characterized. The results showed that the conjugation ratio of SM to BSA in SM artificial antigen was about 25:1. The titer of supernatant hybridoma cell lines of 3F9-C4 was 1:3.2×10^2 by indirect ELISA. The isotypes in ascites was IgG(subscript 2a)/k, the affinity constant was 8.4×10^11L/mol, IC50 was 8.99μg/L, cross-reactivity to dihydrostreptomycin was 109.6% and little cross-reactivity to other compounds.

结果表明，BSA-SM人工抗原分子结合比为1：25；筛选出3F9-C4敏感特异的杂交瘤细胞l株，间接ELISA 刚定细胞培养上清，效价为l：3.2×10^2，同种型为IgG(下标 2a)/k腹水的亲和常数为8.4×10^11L/mol；IC50为8.99μg/L，与双氢链霉素交叉反应为109.6%，与其他SM结构相似物和功能近似物无交叉反应性。

Allen was a researcher at the Department of Neurobiology, The Babraham Institute, Cambridge, UK. Dr. Allen's research skills and techniques include DNA/RNA manipulation and purification/isolation, electrophoreses, PCR, cloning, all blottings, library screening, genetic mapping, design and construction of transgenes, artificial chromosome engineering, design and construction of vectors for gene targeting in ES cells, cell culture, histological techniques, mouse behavioural analysis, small animal surgery, and bioinformatic tools such as sequence databases and analysis tools.

从1996年到 2004年，Dr。 Allen 一直在英国剑桥Babraham 研究所神经生物学部门从事研究工作，包括DNA/RNA操纵、提纯/分离、电泳、PCR、克隆、印迹工作、文库筛选、遗传图绘制、转基因设计与构建、人工染色体工程、用于ES细胞基因打靶的载体设计与构建、细胞培养、切片技术、小鼠行为分析、小动物外科手术以及序列数据库和分析工具等生物信息工具的研究。

In the negative and interrogative forms, of course, this is identical to the non-emphatic forms.

。但是，在否定句或疑问句里，这种带有"do"的方法表达的效果却没有什么强调的意思。

Go down on one's knees;kneel down

屈膝跪下。。。下跪祈祷