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亲和力

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This thesis analyze the TV text and the interview of viewers, in order to realize what the TV text presented, and viewers how to recognize the TV text. With regard to the TV text, it represented the virtual relationship of diffuseness, this is the main element to attract viewers, that the talk show looks like many people who discuss the social event, but it is just chatting.

透过对於电视文本的分析,以及对於个案访谈的分析中发现,该节目透过亲和力的展现而构筑出虚拟的扩散性关系,其中亲和力的展现,主要是透过语言和和谐的情境,此为吸引观看者的元素,也就是该节目所展现的只是如同日常生活中与亲朋好友聊天的情境,对於社会事件的谈论,也并真正提出了建立在学理上的分析,大多只是来宾、主持人陈述自己的心得与对於事件的揣测。

The results showed that garden sass, cole and winter wheat had higher affinity to NO3-N than NH4+-N, but cucumber and soybean had opposite tendency.

结果表明,大青菜、油菜和冬小麦对NO_3~--N的亲和力大于对NH_4~--N的亲和力,而黄瓜、大豆2种作物与此相反。

Monovalent cations decrease the affinity of Mg〓 to the enzyme but don't affect the affinity of Fru-1, 6-P〓.

钾离子的存在使得酶对镁离子的亲和力下降,而对酶与底物的亲和力没有影响。

Citri had been selected by ribosome display in previous research. In order to express the scFvs with bio-activity efficiently, the genes of the selected scFvs were transformed into Escherichia coli HB2151 for expressing resoluble antibodies. The expression levels of scFvs were detected by dot blotting and Western blotting, and the expressed scFvs were purified by affinity chromatography, and then the activity of the purified scFvs was tested by enzyme-linked immunosorbent assay .

Citri, Xac高亲和力单链抗体(Single chain variable fragment, scFv)GX13、GX44和GX95,为高效表达具有生物活性的单链抗体,本研究将前期筛选的高亲和力单链抗体基因转入大肠杆菌HB2151中进行可溶性表达,点印迹和Western印迹检测可溶性单链抗体的表达水平,亲和层析纯化表达的单链抗体,酶联免疫吸附法检测纯化单链抗体的抗原结合活性。

The research work consists of two aspects:the relationship between fluorine in coal and ash,mineral composition;organic affinity and washability of fluorine in coal.

研究内容包括:煤中氟与灰分、矿物成分之间的关系;煤中氟的有机亲和性与可选性;研究结果表明:煤中氟质量分数与灰分质量分数之间呈正相关关系;中国煤中氟质量分数与灰分之间存在以下指数增长关系;煤中氟质量分数与煤中矿物质的成分有关;煤中氟属"中等无机型"元素,无机亲和力较强,有机亲和力较弱;煤中氟以无机氟为主,有机氟为辅的方式赋存;原煤洗选是脱除煤中氟的一种可行的技术措施。

E. , ACTH antagonizes the analgesia mediated byμand δ opioid receptors, but notκreceptor;(2) The antagonizing effect of ACTH on opioid analgesia is proposed to be mediated by ACTH receptors, although the latter has not been characterized;(3) A contradictory interaction on intracellular cAMP content may constitute one of the postreceptor mechanisms underlying ACTH antagonism of opioid analgesia;(4) Another proposed mechanism of the anti-opioid effect of ACTH is that ACTH can modulate opioid-induced suppression of calcium influx;(5) ACTH has been shown to induce Fos protein expression in selected areas of the rat brain including the nuclei involved in pain regulation as well as the ependyma of the third ventricle and the aqueduct.

根据以上实验结果,本论文首次提出以下论点:(1)ACTH在脊髓水平对抗阿片镇痛具有受体选择性,即ACTH可对抗μ受体和δ受体介导的镇痛,不对抗κ受体所介导的镇痛;(2)由于ACTH与阿片μ受体的肽类配体的结合位点仅有很低的亲和力,与μ受体的非肽类配体的结合位点以及δ受体无亲和力,推测ACTH是通过中枢内的ACTH受体介导发挥抗阿片效应的;(3)ACTH抗阿片作用的受体后作用机制之一是在cAMP信使通路水平与阿片发生相互作用;(4)ACTH抗阿片作用的另一受体后机制是在〓水平影响阿片的效应;(5)通过Fos蛋白的诱导揭示:ACTH可以作用于脑内多个核团,其中包括许多与痛觉调制有关的核团,推测ACTH可能通过激活这些核团的神经元而发挥其中枢效应。

ELISA and immunofluorescence assays showed that ER had strong affinity for A431 and A549 cells that overexpress EGFR.

ELISA检测及细胞免疫荧光显示融合蛋白与纯EGFR抗原的相对亲和力为8.0×10~(-9)M,对EGFR高表达的肿瘤细胞的亲和力明显高于低表达的正常细胞。

It was found that La〓 does not affect the binding affinity between calmodulin and Polistes Mastoparan, a known calmodulin binding peptide, neither the conformation of the ternary complex. Excessive amount of La〓 result in the decrease of the binding constant and the disrupted conformation. The binding affinity of La〓 to calmodulin increased in the ternary complex (La〓-CaM-Mas/Mas X), which suggested the coordination between the two global domains. The binding priority between the two global domains is also changed: La〓 more is likely to bind to the C-terminal of calmodulin than to N-terminal, thus facilitates the binding of Mas/Mas X to the C-terminal of calmodulin as the first step of the Mas/Mas X binding.

在以钙调蛋白结合肽—Polistes Mastoparan和Mastoparan X为对象的研究中,我们发现,除非过量,否则La〓的存在不影响钙调蛋白与Mas间的结合常数以及构象;过量的La〓(La〓/CaM摩尔比>4)将引起钙调蛋白结合功能的下降,同时明显改变金属-钙调蛋白-Mas三元复合物的构象;La〓对Ca〓CaM-Mas的N末端表现高度选择性,显示了在三元复合物中两种离子间的协同效应;在三元体系中,La〓与钙调蛋白的亲和力明显上升,而且亲和力上升的程度因钙调蛋白结合肽的不同而有明显差异,显示了作用的选择性;Mas和MasX的存在改变了La〓在钙调蛋白上的结合顺序,La〓很可能优先与C末端结合,从而使Mas/Mas X首先与C末端结合,该顺序与钙离子相同;La〓的参与使三元复合物在动力学上更加稳定。

Current understanding of the kinetics of IgE binding to high-affinity receptors on mast cells and basophils is imperfect, but available data suggest that disarming high-affinity receptors on these cells is likely to take weeks rather than hours or days.

目前对IgE与效应细胞表面高亲和力受体结合的动力学状况尚不清楚,但已有的资料表明使这些高亲和力受体失活可能需要几周时间,而不是几个小时或几天。

The inhibitory potency of these chelators on ryanodine inding is consistent with their affinity with 〓.

它们的抑制作用和它们与锌离子的亲和力一致,即亲和力越高,抑制作用越强。

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