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Second, the segregation analysis was applied to determine inheritance mode of litter size in Inner Mongolian cashmere goat.

第二,应用分离分析法确定内蒙古绒山羊胎产羔数的遗传方式。

The study aimed to explore the genetic law of litter size of Inner Mongolia White Cashmere Goat.

[目的]探讨内蒙古白绒山羊产羔数的遗传规律。

Polymorphism was detected for exon 3 of BMP4 gene in four sheep breeds. For exon 3, three genotypes were detected in four sheep breeds. In Small Tail Han, Chinese Merino, Corriedale and South African Mutton Merino sheep, frequency of genotype AA was 0.017, 0.216, 0.115, 0.429, frequency of genotype AB was 0.102, 0.317, 0.269, 0.500, frequency of genotype BB was 0.881, 0.467, 0.616, 0.071, respectively. Sequencing revealed one singlenucleotide mutation C→A at 305 bp of exon 3 of BMP4 gene in genotype BB in comparison to genotype AA, and this mutation resulted in an amino acid change of alanine→aspartic acid.

对于外显子3的引物,在4个绵羊品种中都检测到3种基因型;统计结果表明:小尾寒羊、中国美利奴绵羊、考力代绵羊、南非肉用美利奴绵羊AA基因型频率分别为0.017、0.216、0.115、0.429,AB基因型频率分别为0.102、0.317、0.269、0.500,BB基因型频率分别为0.881、0.467、0.616、0.071;测序结果表明:BB型和AA型相比在BMP4基因外显子3第305位碱基处发生C→A的突变,该突变导致氨基酸由丙氨酸改变为天冬氨酸;BB型小尾寒羊平均产羔数分别比AB型和AA型多0.61只(P.05)和1.01只(P.05)。

In Small Tail Han sheep, Chinese Merino sheep, Corriedale sheep and South African Mutton Merino sheep, frequency of AA genotype was 0.58, 0.98, 0.96, 1.00, frequency of AB genotype was 0.40, 0.02, 0.04, 0.00, frequency of BB genotype was 0.02, 0.00, 0.00, 0.00, respectively. The ewes with mutation homozygous genotype BB or mutation heterozygous genotype AB had 1.45 at 305 bp of exon 3 of BMP4 gene in the BB genotype in comparison to the AA genotype, and this mutation resulted in an amino acid change: alanine—?aspartic acid. The ewes with genotype BB had 0.61 P

结果表明:小尾寒羊、中国美利奴绵羊、考力代绵羊在INHA基因5'调控区发生了1处碱基突变(316C→T),南非肉用美利奴绵羊没有发生这种突变;小尾寒羊、中国美利奴绵羊、考力代绵羊、南非肉用美利奴绵羊AA基因型频率分别为0.58、0.98、0.96、1.00,AB基因型频率分别为0.40、0.02、0.04、0.00,BB基因型频率分别为0.02、0.00、0.00、0.00;突变纯合型和突变杂合型小尾寒羊平均产羔数分别比野生型多1.45只P

Here is a pic of Bill Lambing's beautiful TREPANG which shows this clearly.

这里是一个事先知情同意的条例草案,产羔的美丽海参这表明这清楚。

This feed is of special value in steaming up the ewes as they come to lambing.

这种饲料对即将产羔的母羊补充营养很有用。

The polymorphism information content were 0.6674, 0.6035, and 0.5615, respectively. The total mean of litter size of genotype LSCV043 107 bp/123 bp was significantly higher than that of genotype 110 bp/123 bp. The liner size of the first lambing for genotypes BMS2508 154 bp/154 bp, 154 bp/170 bp and 154 bp/200 bp were significantly higher than that of genotype 170 bp/170 bp (P.05). Furthermore, total litter size for BMS2508 170 bp/170 bp was the lowest in all the genotypes within this locus (P.05). No significant difference was detected in other genotypes.

对不同基因型群体的产羔率进行统计分析,LSCV043基因型为107 bp/123 bp所对应的总体产羔数明显高于110 bp/123 bp基因型群体所对应的产羔数(P.05);BMS2508基因型为154 bp/154 bp、154 bp/170 bp和154 bp/200 bp所对应的群体第一胎产羔数明显高于170 bp/170 bp所对应的产羔数(P.05),基因型170 bp/170 bp的群体总体产羔数均低于该位点的其他基因型(P.05);其他位点各基因型之间产羔数均无显著差异。

In the genetic analysis models, non-genetic factors were main effects of birth year, birth month, lambing year, lambing month, age and parity. Birth year, age, lambing year and parity had significant influence on litter size and prolificacy.

其中被筛选的固定效应为出生年份(11个水平)、出生月份(2 个水平)、产羔年份(14 个水平)、产羔月份(3 个水平)、年龄(8 个水平)和胎次(8 个水平)等6 个非遗传因素的主效应。

These results intensively demonstrated that BMPR-IB gene is a major gene that affects the prolificacy in both Small Tail Han sheep and Hu sheep, and could be used as a molecular genetic marker to select for litter size in sheep.

BMPR-IB基因型在几个绵羊品种之间的分布以及各种基因型绵羊的实际产羔两方面的研究结果都证明BMPR-IB基因是影响小尾寒羊和湖羊高繁殖力的一个主效基因,可以用于对绵羊产羔数的辅助选择。

Vagina sponge bolt and CLO was used in oestrous synchronization of donors and acceptances , the rates of estrus was100.0% and 83.0%. 6mg FSH and CLO was used in superovulation of Hexi Fine wool goat,115 embryos were acquire totally and average14.4 embryos,100 useful embryos, average 12.5 embryos.The surgical operation used in embryos transplant, the rate of Gestation was 82.4%, 47 lambs was birth,and the birthrate of lamb was 167.9%.

采用阴道海绵栓结合氯前列稀醇法对供、受体进行同期发情处理,处理结果的发情率分别为100.0%和83.0%;采用 FSH剂量6m g/只结合氯前列稀醇对河西绒山羊超数排卵,共回收胚胎115枚,头均14.4枚,可用胚胎100枚,头均12.5枚;采用手术法对杂种河西绒山羊受体移植后,妊娠率82.4%,产羔47只,产羔率为167.9%。

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The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应,而且减少跟风的可能性。

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