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A strain library containing 226 bacterial and fungal xylanase producers was successfully constructed, two bacterial producers Pseudomonas sp WXULI-11 and WL-11 and three fungal producers Aspergillus niger XY04B, Aspergillus niger XY07C and Aspergillus glaucus XY12D were screened out from this library.

建立了一个包括226株细菌和霉菌木聚糖酶产生菌的小型菌种库,从中筛选出了2株细菌(Pseudomonas sp WXULI-11和WL-11)及3株霉菌(Aspergillus nigerXY04B、Aspergillus niger XY07C及Aspergillus glaucus XY12D),它们的共同点是木聚糖酶生物合成量高,且不产或少产纤维素酶。

In the condition of the above mentioned, field experiments were done. The results showed that:The process was the inoculation proportion of Candida tropicalis, Pseudo monas and Candida utilis as 1:2:2, inoculation 2%, 2.0% urea added in Jowar juice, pH 6.0, fermenting 72h at 30℃. The content of fulvic acid could reach more than 20%.5% mixture of Azotobacter chooroccum, Phospho bacteria and Silicate bacteria (2:1:2) were added to the product. The total number of bacteria reached 10 /ml.

研究结果表明:用热带假丝酵母、假单胞杆菌和产朊假丝酵母组合进行液体发酵,接种比例1∶2∶2,接种量2%,在甜高粱秸秆汁中加入2.0%的尿素,pH值为6.0,发酵温度为30℃,发酵72h,发酵产物中生化黄腐酸含量可达20%以上;将圆褐固氮菌、磷细菌、钾细菌以2∶1∶2的比例,按5%加入到发酵产物中,三种细菌总的活菌数可达10~8个/ml;生化黄腐酸复合生物液肥对各种作物的产量均有不同程度的提高,经济效益显著。

Results The percentage of produced metallo-β-lactamase, lossed outer membrane OprinD2 and active efflux system in P A resistance to carbapenem are 29.8%, 31.9% and 31.9%, produced other β-lactamase is 6.4%, Conclusion Metallo-β-lactamase production is one of the main mechanisms in PA resistance to carbapinem, the result of correct detection in laboratory can not only promote the rational application of antimicrobial agents but also prevent the spread of bacterial resistance

结果 产金属酶、D2缺失、外排泵出分别占29.8%、31.9%、31.9%,产其他β-内酰胺酶占6.4%。结论产金属酶是PA耐碳青酶烯类抗菌药物最主要机制之一,准确的实验室检测不仅可以帮助临床合理选用抗菌药物并可减少细菌耐药性的传播。

The paper reviewed the current research progress and the main achievements of biohydrogen production technology research both at home and abroad,and described the hydrogen production mechanism s of main hydrogen producing organisms,such as phot.

本文综述了目前国内外生物制氢技术各个方面的研究进展和成果,并详细描述了光合产氢细菌及发酵产氢细菌等主要产氢生物的产氢机制。

Anaerobic synecology of the hydrogen-producing fermentation system was investigated under certain test condition in this thesis. In the continuous flow system of anaerobic hydrogen-producing fermentation, the succession of hydrogen-producing acidogenic microbiology may be regressive process. By regressive process we means that the original community getting from nature which structure being complex with a high ability would degenerate gently to a certain climax community which structure being simple with less ability.

产氢发酵系统的微生物群落生态学研究证明,在连续流发酵产氢反应系统中产氢产酸发酵细菌群落的演替过程,是在人为控制的生境条件下,微生物从自然群落这种稳定性大、组成及生化反应过程复杂的生态群落,向稳定性小、组成和生化反应过程相对简单的某一产酸发酵顶极群落进行的"逆行演替"。

Results The total intestinal bacteria of Grass carp of the control group was 6.6×10^8 CFU/g ,and the dominant flora included Aeromonas, Pseudomonas and Enterobacter; After 10 d feeding on the concentration of 2.5%, 5.0% and 7.5% Chinese herb medicine compound, the total intestinal bacteria reduced to 3.0×l0^7 CFU/g, 5.0×10^6 CFU/g and 1.5×10^6 CFU/g respectively, and the dominant flora changed to F. indologenes P. alcaligenes and X. campestris.

结果 对照组鱼种肠道内的细菌总数为6.6×10^8CFU/g,其优势菌群为气单胞菌、假单胞菌和肠杆菌;投喂2.5%、5.0%和7.5%中草药复方药饵10d后,其肠道内细菌总数均有所减少,分别为3.0×10^7CFU/g、5.0×10^6CFU/g和1.5×10^7CFU/g,其优势菌群为吲哚黄杆菌、产碱假单胞菌和野油菜黄单胞菌。

Methods Two arrays of isolates were used to evaluate the reliability of ChromID ESBL. One array contained 285 clinical isolates in which the ESBLs-producing feature was screened by confirmatory tests recommended by Clinical and laboratory Standards Institute. The other array contained 28 enterobacterial isolates with their ESBLs gene and producing enzyme feature being confirmed previously.

用2组肠杆菌科细菌进行评估,一组为285株上海地区检出的临床分离株,这些菌株已用美国临床实验室标准化研究所推荐的确证试验确认了是否产ESBLs,另一组28株产ESBLS肠杆菌科细菌,在以前的研究工作中已确认了这些菌株的产酶特性和ESBLs。

The biotransformations of isoeugenol to vanillin with various strains available in our lab were tested. It was found that several baterica including Bacillus subtilus 7658, Alcaligenes AS1.7617, Brevibacterium flavum 1.7a and Brevibacterium flavum AS1.013 could convert isoeugenol to vanillin.

对实验室所保藏的细菌、放线菌、真菌进行初步筛选发现细菌中枯草芽孢杆菌7658、粪产碱杆菌AS1.7617、黄色短杆菌1.7a、黄色短杆菌AS1.013能产生少量香草醛,最高浓度为0.31 g/L;放线菌中诺卡氏菌Nocardia sp。

The effects of nutrition condition on the activation of stratal microflora, especially the beneficial bacteria including hydrocarbon degrading bacteria, methaneproducing bacteria and nitrate reducing bacteria for oil recovery, were investigated on the basis of the analysis of the stratal microflora in S12 block in Shengli Oilfield.

在考察了胜利油田 S12块注入水和油井产出水中细菌群落状况的基础上,针对烃类降解菌、产甲烷菌、硝酸盐还原菌等有益于采油的细菌群落,研究了营养条件对细菌群落激活的影响。

This thesis put that, although ETF and BATF had the same biogas yield, their hydrogen yields were remarkably different in the same testing condition because the hydrogen content in their produced biogases were different due to the difference of their metabolic pathways.

本研究先后从连续流反应系统的乙醇型发酵阶段和丁酸型发酵阶段分别7次采集分离样品,从中分离到215株产酸发酵细菌,其中有36株具有产氢能力。

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According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm~150rpm时取得最大值,此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世,形体几无变化。

When I was in school, the rabbi explained everythingin the Bible two different ways.

当我上学的时候,老师解释《圣经》用两种不同的方法。