产生酶原的

BACKGROUND : By now , safety of collagenase application is still controvertible and some scholars believed that collagenase might induce the peripheral tissue injury .

背景：目前对于胶原酶应用的安全性仍有不同观点，有学者认为胶原酶可能会对周围组织产生损伤。

The signal peptides of chicken Igλ light chain and murine plasmingen were fused to the GFP N terminus using site-directed mutagenesis to construct the vectors with signal sequence, pEGFP-SPc and pEGFP-SPm. The chicken Igλ gene was inserted into the two vectors with signal sequence after double enzyme cutting.

分别将鸡Igλ轻链信号肽、小鼠纤溶酶原信号肽与pEGFP-C1载体的绿色荧光蛋白N端融合，产生带有信号肽的中间载体pEGFP-SPc和pEGFP-SPm。

The distribution of ABH substances in humantissue cells of nonsecretors differs from those insecretors.the contents of ABH in cells of nonsecretorswere much less than those of secretors.ABH substanceswere demonstred in cells of deep part of gastric gland,Brunner gland of duodenum,acini and duct of pancreas,acini and duct of sweat gland and in endothelial cells ofblood vessels and heart.

在国内外首次用免疫透射电镜对正常人体的胃、十二指肠、横结肠的粘膜上皮和腺上皮的ABH物质进行亚细胞定位研究后发现，ABH物质分布于粘膜上皮及粘液性细胞的Golgi器、粘原颗粒和胃腺主细胞的酶原颗粒中，证实人体组织细胞的ABH物质由自身产生，而不是从血液吸附。

Additionally, we have explored the proteolysis mechanism of angiogenesis inhibitor production in bacteria system.

另外，我们还通过细菌系统对蛋白酶水解纤溶酶原产生血管生成抑制因子的机理进行了研究。

Want the skin to still can produce new collagen only, cuticular collagen is enzymatic build in responsible compose crudely collagen while also adjust of skin collagen form; Cut generation collagen is enzymatic.

只要皮肤还能产生新的胶原质，表皮胶原酶就在负责构建未成熟的胶原质的同时也调节皮肤胶原质的形成；伤口产生胶原酶。

Phenylmethylsulfonyl fluoride (174.2 mg/mL), chicken ovomucoid (1000 mg/mL) and soy-bean trypsin inhibitor (1000 mg/mL) could inhibit enzyme activity, which indicated that the enzyme belonged to serine protease group. On plasminogen-free fibrin plates and plasminogen fibrin plates, the fibrinolytic activity had no obvious difference, indicating that the enzyme was a fibrinolytic enzyme which degraded fibrin directly, but not a plasminogen activator which degraded fibrin by activating plasminogen.

此菌株产生的纤溶酶在50℃以下和pH5.0~11.0范围内具有较好的稳定性，最适作用温度为42℃；最适pH值为9.0；Mg2+、Ca2+对此酶有明显的激活作用，而Cu2+能完全抑制酶的活性；174.2 mg/mL的苯甲基磺酰氟、1000 mg/mL的鸡卵类粘蛋白和1000 mg/mL大豆胰蛋白酶抑制剂能完全抑制酶活性，初步说明此酶属于丝氨酸蛋白酶类；体外溶纤作用表明，该酶溶解纤维蛋白的方式是直接溶解，而不是通过激活纤溶酶原。

The pathogen of gas gangrene mainly exist and live in local place, and seldom enter blood system to cause blood poisoning, but than can form lots of toxins, this toxins can harm blood system, kidney system, and also the tissue can putrescence and the wound will enlarge step by step, all tissue putrescence and toxin can make these bacteria easier to live and reproduce, all these situation if not being control in time will lead a serious situation that multiple organs dysfunction and then died

气性坏疽的病原菌主要在伤口内生长繁殖很少侵入血液循环引起败血症。产气夹膜杆菌产生α毒素、胶原酶透明质酸酶、溶纤维酶和脱氧核糖核酸酶等，红细胞破坏引起溶血血红蛋白尿、尿少、肾组织坏死水肿、液化，肌肉大片坏死使病变迅速扩散、恶化。糖类分解产生大量气体使组织膨胀；蛋白质的分解和明胶的液化，产生硫化氢，使伤口发生恶臭由于局部缺血，血浆渗出，及各种毒素的作用伤口内的组织和肌肉，进一步坏死和腐化，更利于细菌的繁殖使病变更为恶化。

The effects and mechanism of GABAergic neurons, NOergic neurons, opioid peptide and cyclic adenosine monophosphate in the nucleus reticularis thalami on sleep-wakefulness cycle of rats and the effects and mechanism of the 5-HTergic nerve fibers project from the nucleus raphes dorsalis to RT on sleep-wakefulness cycle of rats were investigated with the methods of brain stereotaxic, nucleus spile, microinjection and polysomngraphy.1. The effects of GABAergic neurons in RT on sleep-wakefulness cycle of rats1.1 Microinjection of 3-mercaptopropionic acid (3-MP, a kind of glutamate decarboxylase inhibitor) into RT. On the day of microinjection, sleep only decreased a litter. On the second day, sleep marked decreased and wakefulness marked increased. On the third and fourth day, sleep and wakefulness stages resumed to normal.1.2 Microinjection of gamma-amino butyric acid (GABA 1.0μg) into RT enhanced sleep and reduced wakefulness compared with control; while microinjection of L-glutamate (L-Glu, 0.2μg) decreased sleep and increased wakefulness; microinjection of bicuculline (BIC, 1.0μg), a GABAA receptor antagonist, enhanced wakefulness and reduced sleep; microinjection of baclofen (BAC, 1.0μg), GABAB receptor agonist, had the same effects as GABA.2. The effects of NOergic neurons in RT on sleep-wakefulness cycle of rats2.1 Microinjection of L-arginine (L-Arg, 0.5μg) into RT decreased sleep compared with control, but there were on statistaical difference between L-Arg group and control; while microinjection of sodium nitroprusside (SNP, 0.2μg), a NO donor into RT, sleep marked decreased and wakefulness marked increased. Microinjection of nitric oxide synthase inhibitor, N-nitro-L-arginine (L-NNA, 2.0μg) into RT enhanced sleep and reduced wakefulness.2.2 After simultaneous microinjection of L-NNA (2.0μg) and SNP (0.2μg) into RT, SNP abolished the sleep-promoting effect of L-NNA compared with L-NNA group; after simultaneous microinjection of L-NNA (2.0μg) and L-Arg(0.5μg) into RT, we found that L-NNA could not blocked the wakefulness-promoting effect of L-Arg.3. The effects of opioid peptide in RT on sleep-wakefulness cycle of rats3.1 Microinjection of morphine sulfate (MOR, 1.0μg) into RT increased wakefulness and decreased sleep compared with control; while microinjection of naloxone hydrochloride (NAL, 1.0μg), the antagonist of opiate receptors, into RT, enhanced sleep and reduced wakefulness.3.2 After simultaneous microinjection of MOR (1.0μg) and NAL (1.0μg) into RT, the wakefulness-promoting effect of MOR and the sleep-promoting effect of NAL were not observed compared with control.4. The effects of cAMP in RT on sleep-wakefulness cycle of rats Microinjection of cAMP (1.0μg) into RT increased sleep and decreased wakefulness compared with control; microinjection of methylene blue (MB,1.0μg) into RT enhanced sleep and reduced wakefulness compared with control.5. The effects of the 5-HTergic nerve fibers project from DRN to RT on sleep-wakefulness cycle of rats5.1 When L-Glu (0.2μg) was microinjected into DRN and normal sodium (NS,1.0μg) was microinjected into bilateral RT. We found that sleep was decreased and wakefulness was increased compared with control; when L-Glu (0.2μg) was microinjected into DRN and methysergide (MS,1.0μg), a non-selective 5-HT antagonist, was microinjected into bilateral RT, We found that sleep was enhanced and wakefulness was reduced compared with L-Glu group.5.2 When p-chlorophenylalanine (PCPA, 10μg) was microinjected into DRN and NS (1.0μg) was microinjected into bilateral RT, We found that sleep was increased and wakefulness was decreased compared with control; microinjection of 5-hydroxytryptaphan (5-HTP, 1.0μg), which can convert to 5-HT by the enzyme tryptophane hydroxylase and enhance 5-HT into bilateral RT, could block the effect of microinjection of PCPA into DRN on sleep-wakefulness cycle.

本研究采用脑立体定位、核团插管、微量注射、多导睡眠描记等方法，研究丘脑网状核(nucleus reticularis thalami，RT)中γ-氨基丁酸(gamma-amino butyric acid ，GABA)能神经元、一氧化氮(nitrogen monoxidum，NO)能神经元、阿片肽类神经递质、环一磷酸腺苷(cyclic adenosine monophosphate，cAMP)及中缝背核(nucleus raphes dorsalis，DRN)至RT的5-羟色胺(5-hydroxytryptamine，5-HT)能神经纤维投射对大鼠睡眠-觉醒周期的影响及其作用机制。1 RT内GABA能神经元对大鼠睡眠-觉醒周期的影响1.1大鼠RT内微量注射GABA合成关键酶抑制剂3-巯基丙酸(3-MP，5μg)，注射当天睡眠时间略有减少，第二日睡眠时间显著减少，觉醒时间明显增多，第三、四日睡眠和觉醒时间逐渐恢复至正常。1.2大鼠RT内微量注射GABA受体激动剂GABA( 1.0μg)后，与生理盐水组比较，睡眠时间增加，觉醒时间减少；而RT内微量注射L-谷氨酸(glutamic acid， L-Glu， 0.2μg)后，睡眠时间减少，觉醒时间增加；RT内微量注射GABAA受体阻断剂荷包牡丹碱(bicuculline，BIC，1.0μg)后，睡眠时间减少，觉醒时间增加；RT内微量注射GABAB受体激动剂氯苯氨丁酸(baclofen，BAC，1.0μg)后，产生了与GABA相似的促睡眠效果。2 RT内NO能神经元对大鼠睡眠-觉醒周期的影响2.1大鼠RT内微量注射NO的前体L-精氨酸(L-Arg，0.5μg)后，与生理盐水组对比，睡眠时间略有减少，但无显著性意义；而RT内微量注射NO的供体硝普钠(Sodium Nitroprusside，SNP，0.2μg)后可明显增加觉醒时间，缩短睡眠时间；微量注射一氧化氮合酶抑制剂L-硝基精氨酸(L-arginine，L-NNA，2.0μg)后，引起睡眠时间增多，觉醒时间减少。2.2大鼠RT内同时微量注射L-NNA(2.0μg)和SNP(0.2μg)后与L-NNA组比较发现SNP逆转了L-NNA的促睡眠作用；RT内同时微量注射L-NNA(2.0μg)和L-Arg(0.5μg)后，与L-NNA(2.0μg)组比较发现L-Arg可以增加觉醒而缩短睡眠，其促觉醒作用未能被NOS的抑制剂L-NNA所逆转。3 RT内阿片肽对大鼠睡眠-觉醒周期的影响3.1大鼠RT内微量注射硫酸吗啡(morphine sulfate，MOR，1.0μg)后与生理盐水组对比，睡眠时间减少而觉醒时间增加； RT内微量注射阿片肽受体拮抗剂盐酸纳洛酮(naloxone hydrochloride，NAL，1.0μg)后与生理盐水组比较，睡眠时间增加而觉醒时间减少。3.2大鼠RT内同时微量注射MOR(1.0μg)和NAL(1.0μg)后，与生理盐水组对比，原有的MOR促觉醒效果和NAL的促睡眠效果都没有表现。4 RT内环一磷酸腺苷信使对大鼠睡眠-觉醒周期的影响大鼠RT内微量注射cAMP(1.0μg)后与NS(1.0μg)组比较，睡眠时间增多而觉醒时间减少；RT内微量注射亚甲蓝(methylene blue，MB，1.0μg)后，与NS组比较，睡眠时间增多而觉醒时间减少。5中缝背核投射到丘脑网状核的5-羟色胺能神经纤维对大鼠睡眠-觉醒周期的影响5.1大鼠DRN内微量注射L-Glu(0.2μg)，同时在双侧RT内微量注射NS (1.0μg)后，与对照组(DRN和双侧RT注射NS， 0.2μg)比较，睡眠时间减少，觉醒时间增多；大鼠DRN内微量注射L-Glu(0.2μg)，同时在双侧RT内微量注射二甲基麦角新碱(methysergide， MS， 1.0μg )后，与对照组(DRN注射L-Glu 0.2μg，双侧RT注射NS 1.0μg)比较，睡眠时间增多，觉醒时间减少。5.2大鼠DRN内微量注射对氯苯丙氨酸(p-chlorophenylalanine，PCPA，10μg)，同时在双侧RT内微量注射NS (1.0μg)后，与对照组(DRN和双侧RT注射NS， 1.0μg)比较，睡眠时间增多，觉醒时间减少；大鼠DRN内微量注射PCPA(10μg)，产生睡眠增多效应后，在双侧RT内微量注射5-羟色胺酸(5-hydroxytryptaphan ， 5-HTP， 1.0μg )后，与对照组(DRN注射PCPA 10μg，双侧RT注射NS 1.0μg)比较，睡眠时间减少，觉醒时间增多。

To plasmin; used medicinally in some cases of myocardial infarction and pulmonary embolism.

由链球菌产生的一种酶，通过将血浆酶原转化为血浆酶来溶解血凝块，医学上有时用于心肌梗塞和肺栓塞。

Increase of PAI-1 level in blood results in reduction of plasmin generation and predisposes to the formation of fibrin thrombus.

当血中PAI-1异常增高时，纤维蛋白溶酶原激活子对纤维蛋白溶酶原的激活作用减弱，因而没有足够量的纤维蛋白溶酶产生，结果纤维蛋白在血管中积聚而形成血栓。

In the United States, chronic alcoholism and hepatitis C are the most common ones.

在美国，慢性酒精中毒，肝炎是最常见的。

If you have any questions, you can contact me anytime.

如果有任何问题，你可以随时联系我。