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Protease treatment of the plasma membranes could abolish the binding but NaIO_4 and glycosidase could not, indicating that nsLTP144 bound to plasma membranes protein without carbohydrate moiety. Using the homobifunctional cross-linking regent bissuberate (BS~3) and rice plasma membranes incubated with ~(125)I-Trx-nsLTP144, we identified, after SDS-polyacrylamide gel electrophoresis and autoradiography, a putative protein receptor on the rice plasma membranes with the molecular mass around 60 kDa. NsLTP144 can not trigger extracelluar alkalization in arabidopsis, but can abolish the extracellular alkalization effect of phytopathogen elicitor cryptogein, suggesting that cryptogein and nsLTP144 may bind to the same membrane protein. In vitro pull-down assay showed that nsLTP144 interacted with OsCaM1, a possible extracellular calmodulin, implying that nsLTP144 and OsCaM1 could function in the same signal transduction pathway. These results shed light on revealing the roles of nsLTP in vivo and make it promising to finally characterize the plasma membranes receptor of nsLTP.

发现~(125)I-Trx-nsLTP144、~(125)I-Trx-nsLTP110与水稻细胞质膜均具有特异性结合,而且结合是饱和性的、可被竞争的,符合配体-受体结合的典型特征,同时用于对照实验的蛋白质~(125)I-Thioredoxin没有此特性,表明水稻细胞质膜上存在nsLTP的受体;利用可氧化糖基的NaIO_4和水解糖基的N\'-糖苷酶F处理水稻细胞质膜,再进行结合实验,结合活性几乎不受影响;而利用胰蛋白酶处理细胞膜则使得结合能力几乎完全丧失,表明其受体为没有经过糖基化修饰的蛋白质;利用交联剂BS~3交联配体一受体后,再进行SDS-PAGE分离和放射自显影,结果显示水稻细胞质膜上的nsLTP受体中有一个60kDa的蛋白质可以与nsLTP144发生特异性的结合,可能是其受体;细胞外碱化实验表明,nsLTP144不能促使拟南芥原生质体细胞培养液的细胞外碱化反应,却能猝灭来自植物病原菌的激发子Cryptogein刺激拟南芥原生质体产生的细胞外碱化反应,表明nsLTP和Cryptogein结合细胞膜上相同的位点,保护了植物细胞免受Cryptogein导致的细胞程序性死亡,并诱导系统获得性抗性的产生;体外Pull-down实验表明,nsLTP144和水稻的OsCaM1具有相互作用,暗示了nsLTP144和OsCaM1可能同在一个信号通路上起作用。

The result shows that the primary cause producing pockmark is low quality heating salt and nsuitble deoxizing operations,high oxygen po tential during salt-ath heat treatm et,or nonhomogeneous protective atomosphere in furnace when carburizing or dual-eating gears and SO on,which all belong to corrosions caused by in complete oxidation.

结果表明,"麻点"产生的主要原因是因为盐浴热处理时加热盐的质量和脱氧操作不当、齿轮渗碳或二次加热时炉内保护气氛氧势高或不均匀及炉罐漏气等,它们都属于不完全氧化所产生的腐蚀。

The result shows that ponceau 4R has four obvious fluorescence spectral peaks respectively located at 420,530,635 and 687 nm,each of these fluorescence spectral peaks has different best induced light,and the corresponding fluorescence spectra were listed.

文章分别从理论与实验上对220-400 nm不同激发波长下胭脂红标准溶液的荧光光谱进行了分析,结果表明,胭脂红可以产生较强的荧光,分别在420,530,635,687 nm波长处产生了4个荧光峰,各荧光峰的最佳激发波长也不尽相同,文章给出了相应的荧光光谱图。

In order to compare the results for the three different die gaps, the ratio of the two principal strains is introduced, being min/max, where max and min are the major and the minor principal strains, respectively. Hosford and Caddell [5] have shown that if the absolute value of is greater than a critical value, wrinkling is supposed to occur, and the larger the absolute value of , the greater is the possibility of wrinkling.

为了比较三种不同模具间隙的结果,两种主要张力的比率被介绍-是最小值/最大值,这里最小值/最大值是主要的和次要的张力,分别的, Hosford and Caddell [5] 显示如果他的绝对值比临界值大,起皱应该会产生,越大的绝对值,则越大的起皱产生。

The test results show the principle of variation of pressures on culverts beneath high fill is similar, it is that, even if the embankment is filled up to a higher height, the earth pressures can still partly transfer to the top of culvert, as a result the pressures on the culvert increase nonlinearly with the variation of height of fill and further the pressures are obviously lower than theoretical value by σ=γH.

模型试验和数值模拟计算结果表明,当填土达到一定高度后,高填方涵洞上方将产生明显的拱效应,但由于涵洞填土为破碎的岩土,其拱效应与岩石中开挖洞室上方的拱效应不同,高填方涵洞上方填土产生的"土拱"效应具有不稳定的特点,表现为随着填土高度的增加仍有部分土压力传递到涵洞上,涵顶土压力随填土高度成非线性增加。

Results FPCL contraction is due to the traction force generated by fibroblasts. The traction force is a compound force exerted by extension of cellular pseudopod and contractile structure in cytoplasm.

结果 FPCL的收缩是由成纤维细胞产生的一种牵拉力而引起,该力是一种复合式动力,由细胞突起的延伸运动与细胞浆内的收缩结构共同产生。

Comparing to the dynamics performance of the quondam model, the stabilization time is longer ,the overshoot is more, and concussion comes forth.

仿真结果表明回热器的蓄热效应会对单机的动态特性产生显著影响,将使其稳定时间延长,超调量增加,而且会产生一定的震荡现象。

Our results show that fitting the spectrum of residual function in FT method gives more accurate rCBF than that of the two SVD methods. But FT method takes more computational time to remove recirculation.

实验结果显示争Q用傅立叶转换法,并对残余函数的频谱加以拟合,所得到的rCBF ,较不易产生rCBF低估的现象,但为了去除再循环的部分,则必须花费较多的时间;而奇异值分解法则容易产生rCBF低估的现象。

The results are then remapped to adjust the color balance, noise is added to roughen the look of the bricks, and finally the generated color is passed through a Grid texture node to create the look of mortar between the bricks.

这个结果是重贴图去调整颜色平衡,加的噪点使砖的外观变粗糙,最后产生的颜色是通过一个GRID纹理节点来在砖间产生一个泥灰的外观。

The results show that the additional shearing strain different from the normal compression deformation is generated in asymmetric rolling process. The more the different ratio of diameter,the greater the additional shear...

结果表明:在非对称轧制条件下会产生不同于普通压缩变形的附加剪切变形,而且异径比越大,产生的附加剪切变形也越大。

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