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Protease treatment of the plasma membranes could abolish the binding but NaIO_4 and glycosidase could not, indicating that nsLTP144 bound to plasma membranes protein without carbohydrate moiety. Using the homobifunctional cross-linking regent bissuberate (BS~3) and rice plasma membranes incubated with ~(125)I-Trx-nsLTP144, we identified, after SDS-polyacrylamide gel electrophoresis and autoradiography, a putative protein receptor on the rice plasma membranes with the molecular mass around 60 kDa. NsLTP144 can not trigger extracelluar alkalization in arabidopsis, but can abolish the extracellular alkalization effect of phytopathogen elicitor cryptogein, suggesting that cryptogein and nsLTP144 may bind to the same membrane protein. In vitro pull-down assay showed that nsLTP144 interacted with OsCaM1, a possible extracellular calmodulin, implying that nsLTP144 and OsCaM1 could function in the same signal transduction pathway. These results shed light on revealing the roles of nsLTP in vivo and make it promising to finally characterize the plasma membranes receptor of nsLTP.

发现~(125)I-Trx-nsLTP144、~(125)I-Trx-nsLTP110与水稻细胞质膜均具有特异性结合,而且结合是饱和性的、可被竞争的,符合配体-受体结合的典型特征,同时用于对照实验的蛋白质~(125)I-Thioredoxin没有此特性,表明水稻细胞质膜上存在nsLTP的受体;利用可氧化糖基的NaIO_4和水解糖基的N\'-糖苷酶F处理水稻细胞质膜,再进行结合实验,结合活性几乎不受影响;而利用胰蛋白酶处理细胞膜则使得结合能力几乎完全丧失,表明其受体为没有经过糖基化修饰的蛋白质;利用交联剂BS~3交联配体一受体后,再进行SDS-PAGE分离和放射自显影,结果显示水稻细胞质膜上的nsLTP受体中有一个60kDa的蛋白质可以与nsLTP144发生特异性的结合,可能是其受体;细胞外碱化实验表明,nsLTP144不能促使拟南芥原生质体细胞培养液的细胞外碱化反应,却能猝灭来自植物病原菌的激发子Cryptogein刺激拟南芥原生质体产生的细胞外碱化反应,表明nsLTP和Cryptogein结合细胞膜上相同的位点,保护了植物细胞免受Cryptogein导致的细胞程序性死亡,并诱导系统获得性抗性的产生;体外Pull-down实验表明,nsLTP144和水稻的OsCaM1具有相互作用,暗示了nsLTP144和OsCaM1可能同在一个信号通路上起作用。

EPS composition fermented with strain 7 and Streptococcus thermophilus together was stable and consists of glucose, galactose and rhamnose, but EPS with normal starters of Streptococcus thermophilus and Lactobacillus delbrueckii ssp. bulgaricus does not consist of rhamnose.

产粘7号菌在诱变前后与不产粘嗜热链球菌共同发酵产生的EPS单糖组成不变,都是由葡萄糖、半乳糖和鼠李糖组成;而不产粘嗜热链球菌与不产粘保加利亚乳杆菌产生的EPS不含鼠李糖,只有葡萄糖和半乳糖。

Based on the above-mentioned, two kinds of target glycoclusters with different glycoterminus, flexible linkers and different scaffolds were designed, disaccharide glycocluster (including TM1-TM7) and trisaccharide glycocluster (TM8-TM9) respectively, with the purpose of obtaining glycoclusters with high binding affinity to anti-Gal antibody and thus better inhibiting the HAR.

基于上述,针对HAR产生的机制,以人体中天然存在的anti-α-Gal抗体及B细胞表面的相应受体为靶点,以α-Gal抗原末端的二糖Galα1→3Gal和三糖Galα1→3Galβ1-4GluNAc片段为糖基部分,采用柔性的连接臂(氨基保护的2-乙醇胺和3-巯基丙酸)和刚性的芳香骨架及柔性的脂肪链骨架,设计了两类结构新颖的糖簇分子:即氧苷和硫苷键连接的二糖糖簇TM1-TM7)和三糖糖簇(TM8-TM9),目的在于寻找与anti-Gal抗体及B细胞相应受体有较高结合力的糖簇分子,以便有效地抑制HAR反应的发生。

Caramelization is the oxidation of sugar and the Maillard reaction occurs between amino acids and reducing sugars, normally requiring the addition of heat.

通常果汁中糖氧化则形成焦糖,而梅拉德反应则是在氨基酸和还原性糖之间产生的,这个过程往往需要加热来完成。

Make up 40% sugar juice now, using sugar volume and raw material to compare is 1: 0.7, 0.2% citric acid are added in sugar juice, because watermelon skin organization is harder, can appear candy law to heat with boil make, cast the watermelon skin that cent has cut into to boil make, it is OK to begin big a crucial moment heats, make its fast evaporate moisture, boil raw material to become transparent all the time, when sugar juice concentrates ceaselessly and showing syrup, should be to use small fire to undertake heating, lest produce anxious saccharify to make product darkly Brown, determine sugar juice chroma is achieved 70% when should stop to heat.

现配制40%糖液,用糖量与原料比是1:0.7,在糖液中加入0.2%柠檬酸,因西瓜皮组织比较坚硬,可用一次透糖法加热煮制,把分切好的西瓜皮投进一起煮制,开始可以大火候加热,让其快速蒸发水分,一直煮到原料变得透明,糖液不断浓缩而呈糖浆时,应是用小火进行加热,以免产生焦糖化使产品呈黑褐色,测定糖液浓度达到70%时就应停止加热。

We can get chitin and protein from the mycelium using the waste alkali and waste hydrochloric acid, so as to enhance its comprehensive benefits.7 The project of the comprehensive treatment of the mother liquorFor the reason of recrystallization, a large amount of mother liquor were produced with the production of IA, except for IA, it also contain residua sugar, mineral and colloid, which could prevent the recovery of IA crystal.

利用回收的树脂再生酸、碱从菌丝体中分离出甲壳素和菌体蛋白,提高的菌体的综合效益。7、衣康酸提取母液的综合治理方案由于衣康酸的提取采用的是重结晶法,因此,生产过程中会产生大量的母液,母液中除了含有衣康酸外,还含有残糖、无机离子及少量小分子胶质物质等,这些成分的浓度相对较高,抑制了母液中衣康酸的结晶和提取,导致这部分母液只能排放,不仅使衣康酸的提取率降低,也加重了污水处理的负荷。

Oxidation of the terminal ch2oh group of hexoses without oxidation of the reducing function produces hexuronic acids, hooc--cho.

醛糖分子两端氧化形成糖二酸,hooc-n-cooh,例如酒产生的糖醛酸,hooc-4-cho。

Swithers stops short of saying that the animals in her study were compelled to overeat to compensate for phantom calories.

她说:&因为代糖没有提供给老鼠卡路里要来了的信号,所以老鼠就消耗了更多的卡路里,连锁反应表明之所以产生过剩的卡路里不是因为吸收得多了,而是新陈代谢达不到应有的水平。&

In production of jams and jellies, sweetened fruit juices or purees are concentrated to provide shelf-stability to microorganism growth and to produce the desired firm texture.

在酱和果冻的生产中,加糖的水果汁或果泥浓缩后可以防止微生物生长提高货架期的稳定性,并产生理想的组织结构。

Jenny Craig is pushing Volumetrics, a high-volume, low-calorie strategy.And everyone gives a thumbs-down to processed and sugary carbohydrates, which cause insulin to spike and can lead to more fat and even diabetes.

珍妮克雷格正在推动一个高容量,低热量的体积光效果战略,每个人都反对熟的含糖的碳水化合物,他们这样做会导致胰岛素上升,并可能产生更多的脂肪,甚至引发糖尿病。

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But we don't care about Battlegrounds.

但我们并不在乎沙场中的显露。

Ah! don't mention it, the butcher's shop is a horror.

啊!不用提了。提到肉,真是糟透了。

Tristan, I have nowhere to send this letter and no reason to believe you wish to receive it.

Tristan ,我不知道把这信寄到哪里,也不知道你是否想收到它。