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Protease treatment of the plasma membranes could abolish the binding but NaIO_4 and glycosidase could not, indicating that nsLTP144 bound to plasma membranes protein without carbohydrate moiety. Using the homobifunctional cross-linking regent bissuberate (BS~3) and rice plasma membranes incubated with ~(125)I-Trx-nsLTP144, we identified, after SDS-polyacrylamide gel electrophoresis and autoradiography, a putative protein receptor on the rice plasma membranes with the molecular mass around 60 kDa. NsLTP144 can not trigger extracelluar alkalization in arabidopsis, but can abolish the extracellular alkalization effect of phytopathogen elicitor cryptogein, suggesting that cryptogein and nsLTP144 may bind to the same membrane protein. In vitro pull-down assay showed that nsLTP144 interacted with OsCaM1, a possible extracellular calmodulin, implying that nsLTP144 and OsCaM1 could function in the same signal transduction pathway. These results shed light on revealing the roles of nsLTP in vivo and make it promising to finally characterize the plasma membranes receptor of nsLTP.

发现~(125)I-Trx-nsLTP144、~(125)I-Trx-nsLTP110与水稻细胞质膜均具有特异性结合,而且结合是饱和性的、可被竞争的,符合配体-受体结合的典型特征,同时用于对照实验的蛋白质~(125)I-Thioredoxin没有此特性,表明水稻细胞质膜上存在nsLTP的受体;利用可氧化糖基的NaIO_4和水解糖基的N\'-糖苷酶F处理水稻细胞质膜,再进行结合实验,结合活性几乎不受影响;而利用胰蛋白酶处理细胞膜则使得结合能力几乎完全丧失,表明其受体为没有经过糖基化修饰的蛋白质;利用交联剂BS~3交联配体一受体后,再进行SDS-PAGE分离和放射自显影,结果显示水稻细胞质膜上的nsLTP受体中有一个60kDa的蛋白质可以与nsLTP144发生特异性的结合,可能是其受体;细胞外碱化实验表明,nsLTP144不能促使拟南芥原生质体细胞培养液的细胞外碱化反应,却能猝灭来自植物病原菌的激发子Cryptogein刺激拟南芥原生质体产生的细胞外碱化反应,表明nsLTP和Cryptogein结合细胞膜上相同的位点,保护了植物细胞免受Cryptogein导致的细胞程序性死亡,并诱导系统获得性抗性的产生;体外Pull-down实验表明,nsLTP144和水稻的OsCaM1具有相互作用,暗示了nsLTP144和OsCaM1可能同在一个信号通路上起作用。

Blocking tonic GABAA receptors with the additionof 100 M picrotoxin produced an additional 40% reversalof the thiopental-induced depression, but no additional reversalwas seen for isoflurane-depressed responses.

再添加100M印防己毒素阻断持续性GABAA受体可使硫喷妥产生的抑制再多逆转40%,但对异氟烷产生的抑制没有进一步的逆转作用。

If there is an input rotation rate on the vibrator,an induced voltage proportional to the angular rate will be produced in the direction perpendicular to the plane composed by the polarization axis and the input angular rate axis according to the Coriolis effect.

由PZT薄膜材料做成的平板沿厚度方向极化并受电激励产生厚度伸缩振动,当一输入角速度作用于振子,根据哥氏效应,则在极化轴和输入角速度轴组成的平面垂直方向上产生一正比于输入角速度大小的感应电压。

After a review of the social factors of China's preemption, it has found that the development of commodity economy is the root cause and lineage morality is a direct cause, in addition to the active role of social transformation.

通过考察我国先买权产生和发展的社会基础,发现商品经济的发展是其产生的根本原因,宗法文化的深入是直接原因,而社会结构的变迁也发挥了积极作用。

The role of the oxidant used was considered to be a quencher of the semiquinone radical anion of HA to its parent quinone to increase the relative concentration of HA so that the generation of SO~3^- via the electron transfer interaction between Hypocrellins and SO~3^2^- was accelerated.

氧化剂在这一反应中的作用为:将产生的竹红菌素半醌负离子自由基氧化至其母体醌,增加反应物的相对浓度,同时使竹红菌素与SO~3^2^-之间的电子转移循环进行,加速SO~3^-的产生。

Radon is the crust of rock, soil radioelement arising from the decay of a radioactive inert gases, which are mainly the lungs and bronchial have on the human body radiation.

氡是地壳岩石、土壤中放射性元素衰变而产生的一种放射性惰性气体,它主要是对人体的肺和支气管产生辐射作用。

The main causes of the vibration in pipeline system are in two respects : The first is the vibrant force of the medium acted on pipeline system,i.e.exciting signal,such as the vibrant force caused by the gas flow at the compressor ′ s inlet and outlet and at the ramified and nodel points as well as the action of pig passing through pipeline bridge,etc.;The second is the response to the exciting signal,which is relating to the characteristics of the pipeline system,i.e.the rigidity,support,quality and equipments of the system besides the waveform of the exciting signal.

管道系统振动的原因主要有两方面,一是介质对管道系统产生的振动力,即激发信号,如气流在压缩机进、出口,分支节点部位产生的振动力,清管器通过管桥时对它的作用等;另一方面是对激发信号的响应,其响应除了和激发信号的波形有关外,还和管道系统本身的特征,即系统的刚度及支撑,质量及其配置等情况有关。

The foundation of wharf apron space was improved by use of special rammer of small base area,gradual tamping energy and dynamic replacement .

强夯置换法综合了强夯加固和复合地基的优点,经济有效,但其夯击能在地基中产生的波场对周围建筑物产生着冲击作用,常常制约着其应用。

This paper introduces the generating mechanism, theoretical calculation and lab test of self-excited wave.

介绍了自激压力波产生的机理、理论计算及室内试验情况,自激压力波频率小、幅值大,可对油层产生连续波动作用。

This article especially pointed out following, investment fund isn't providedwith spontaneous tendency to stable capital market, but along with expansion of itsscale and on condition of games between fund management companies, it can playobjective role in stabling capital market.

本文特别指出的是,基金在本性上并不具有稳定资本市场的自发倾向,但是随着投资基金规模的扩大,基金在基金管理人之间产生搏弈的时候,却能对资本市场的活动产生稳定的客观作用。

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In the negative and interrogative forms, of course, this is identical to the non-emphatic forms.

。但是,在否定句或疑问句里,这种带有"do"的方法表达的效果却没有什么强调的意思。

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