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The results showed that the optimized content of Mg~(2+) was 5mmol/L; dNTP was 200μmol/L; Primer was 0.5μmol/L; Taq polymerase was lU/50ul in the PCR system. Only one fragment of about 270bp was amplified within the genome of 12 wild species and 23 cultivars of Mallus Mill., which indicated that Ty1-copia-like retrotransposons were archaic components of apple genome. The nested insertion of near and within the reverse transcriptase gene of Ty1-copia-like retrotransposons wasn\'t found in apple genome.2 The reverse transcriptase conservative region of Ty1-copia-like retrotransposons were amplified from Gala apple using the optimized PCR system. The amplification product was isolated, cloned and sequenced. Forty-five clones containing reverse transcriptase conservative region were obtained (accession number: AY849580 -AY849592 and DQ105035-DQ105066). Cluster analysis of these sequences showed a great heterogeneity among RT domains isolated from the same genotype.

从苹果属12个野生种和苹果23个栽培品种中都扩增到了约270bp的目的产物,且所有试材的扩增结果一致,均无目的条带以外的产物扩增,说明Ty1-copia类逆转座子在苹果属植物中广泛存在,且存在历史可能较为久远;从扩增结果看不出该逆转座子的逆转录酶基因或附近区域在苹果基因组内有嵌套行为的发生。2、利用优化了的PCR方法从嘎拉苹果基因组克隆了45条Ty1-copia类逆转座子逆转录酶保守序列(登陆号分别为AY849580-AY849592和DQ105035-DQ105066),结果表明,同一基因组来源的这些序列存在高度的异质性。

In addition, the metabolizing mechanism of the metabolizable enzymes of resveratrol were discussed in the research .

在两种酶的粗酶反应体系中还存在白藜芦醇代谢的中间产物或其他产物

In addition, the metabolizing mechanism of the metabolizable enzymes of resveratrol were discussed in the research .

在两种 的粗反应体系中还存在白藜芦醇代谢的中间产物或其他产物

The methanolysis of soybean oil and waster cooking oil bylipase-catalysis were not significant different, which showed that lipase was an effectivecatalyst on transesterification of oils having high concentration of free fatty acids.

其次,对副产物甘油的生物利用进行了研究,分别考察了在厌氧和微氧条件下,间歇发酵和批式流加发酵中工业级和试剂级原料对菌体生长及产物合成的影响。

Abnormally enhanced absorptions for the methine proton in products of triaryl orthoformate derivatives were also observed in C6D6 , indicating the formation of the product resulted in radical pair intermediate.

在氘苯中,产物三苯基原甲酸酯衍生物的次甲基质子的异常增强吸收,表明该产物由中性自由基对形成。

Methylglyoxal is a toxic endogenous substance known to react with proteins to form advanced glycation endproducts, which accumulated in the body during aging. Hyperglycemic can accelerate the accumulation of AGEs, and it has been link to the development of diabetic complications.

由於Methylglyoxal在体内会形成高阶糖化终产物,而这些终产物在老化过程中会大量蓄积且高血糖会加速其蓄积,而与糖尿病晚期的并发症有相当的关联性。

Secondly, the silver based catalysts modified by phosphorus, zirconium, zinc, potassium and so on using molecular sieve as carrier, which were produced by ion-exchange method, had also good catalytic performance for 1,2-propane glycol oxidation to methylglyoxal. The conversion of 1,2-propane glycol and the selectivity of methylglyoxal were both high, and the impurity such as formaldehyde and acetaldehyde in the product is little. At the condition of the temperature 300 ℃, the molar ratio of oxygen to 1,2-propane glycol 1.7:1, the space velocity 6000h~(-1), and the concentration of 1,2-propane glycol 70wt%, the conversion of 1,2-propane glycol is 90% and the selectivity of methylglyoxal is 45% on the Ag-P catalyst.

其次对采用离子交换法制备的负载在分子筛上的银系催化剂进行了研究,发现此催化剂在助催化剂磷、锆、锌、钾等的作用下对丙二醇的低温氧化脱氢具有很高的活性,可以得到较好的丙酮醛的收率,并且产物中的甲醛、乙醛副产物的量比较低;经过对Ag-P催化剂上反应条件的考察,发现当温度为300℃、氧醇比1.7:1、空速6000h~(-1)、丙二醇浓度为70wt%时,丙二醇的转化率为90%,丙酮醛的选择性为45%。

The results indicated isoprene was soon consumed under the condition of excess ozone. During the reaction, the major carbonyl products formed were methyl vinyl ketone, methacrolein, methylglyoxal, and formaldehyde. Formic acid, CO and CO2 were also among the products.

在反应温度(294±2)K,压力为1.01×10^5Pa空气,O3过量的条件下,异戊二烯很快反应完全,反应中生成的羰基产物主要包括甲基丙烯醛、甲基乙烯基酮、丙酮醛和甲醛;产物中还包括甲酸、一氧化碳和二氧化碳。

The main gluconeogenic precursor in kidney is thought to be lactate; however, less is emphasized enantiomerically. L-lactate is a glycolysis end-product, but D-lactate is formed after detoxification of methylglyoxal, which is the main source of advanced glycation end-products.

乳酸为肾脏糖质新生的主要来源,其含有一不对称碳,故具有D-、L-乳酸两种镜相异构物,而D-、L-乳酸两者之生成相当不同,L-乳酸是糖解作用之终产物,D-乳酸为体内一醣化终产物(advanced glycation end-products)─甲基乙二醛进行去毒化反应所生成,目前缺乏对乳酸镜像异构物与肾脏糖质新生间相关的探讨。

The experimental results showed that, monochloroacetic acid and dichloroacetic acid enhanced photocatalytic hydrogen generation activity, but trichloroacetic acid did not improve it. The amount of produced H_2 increases almost proportionally to the irradiation time within 5 h irradiation. The photocatalytic oxidation of monochloroacetic acid and dichloroacetic acid mainly produced CO_2, HC1 and CH_2O, the photocatalytic oxidation of trichloroacetic acid mainly produced CO_2 and HCl.

实验表明,一氯乙酸、二氯乙酸的存在明显地提高了制氢反应效率,而三氯乙酸的存在却不能起到促进作用;反应5h内,反应生成氢的量与反应时间成线性关系;一氯乙酸、二氯乙酸光降解产物主要是CO_2、HCl和CH_2O,三氯乙酸光降解产物主要是CO_2和HCl;研究了溶液pH值对放氢反应的影响;在实验浓度范围内,一氯乙酸、二氯乙酸浓度对放氢反应的影响,表观上符合Langmuir-Hinshelwood关系式;考察了一氯乙酸、二氯乙酸在光催化剂表面的吸附。

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随着死亡的吉他手Schuldiner接受主唱的职务,乐队在现实中树立了重要的影响。

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关闭眼睛,深呼吸,一切不再是梦想,犹如。。。。。。