产物

Investigation of major metabolic pathways of SFZ-47 in animals The metabolites of SFZ-47 in rabbit urine following oral administration were separated and detected with HPLC and LC-MS methods. On the basis of the chromatographic behavior and mass spectra of the metabolites as well as biotransformation pathways of drugs with the partial structure of SFZ-47, the hydroxylic derivative 4-(3H-1, 2- dihydro-1-pyrrolizinone-2-methylamino benzyl alcohol and the carboxylic derivative 4-(3H-1, 2-dihydro-1-pyrrolizinone-2-methylamino benzoic acid of SFZ-47 were proposed as two potential metabolites and were synthesized.

在动物体内主要代谢途径的确定用HPLC和LC-MS对家兔口服SFZ-47后尿中的代谢物进行了分离、检测，根据代谢产物的色谱和质谱行为以及与SFZ-47有类似结构部分的其它药物的代谢途径，推测其羟基衍生物[4-（3H-1，2-二氢-1-吡咯里嗪酮-2-甲基胺基）苯甲醇]与羧基衍生物[4-（3H-1，2-二氢-1-吡咯里嗪酮-2-甲基胺基）苯甲酸]是两种可能的代谢产物，遂用化学方法合成了这两种衍生物。

The N gene ORF was then subcloned into pET-30a vector and the recombinant plasmid was transformed into E.coli BL21 (DE3) and induced with IPTG. The protein expression was determined by SDS-PAGE. The expressed protein had a molecular weight of 54.4 Ku that existed as inclusion body. Thin-layer scanning showed that the expression product accounted for 30.5% of the total bacterial proteins. The recombinant protein possessed native biological activity and could react with anti-PEDV hyperimmune serum in Western blot.

以阳性质粒为模板，用分别含有BamH Ⅰ和Xho Ⅰ酶切位点的上、下游引物扩增得到ORF，其PCR产物经BamH Ⅰ和Xho Ⅰ双酶切后定向克隆到pET-30a载体，构建的重组质粒命名为pET-30a-PN；将pET-30a-PN转化到大肠杆菌BL21 (DF3)中，在IPTG诱导下进行表达；SDS-PAGE结果表明表达出与预期大小相符的约54.4 Ku的重组蛋白，重组蛋白以包涵体形式存在；薄层扫描结果表明表达产物占菌体总蛋白的30.5%；Western blot分析表明表达的重组蛋白能与抗PEDV高免血清反应，说明该重组蛋白具有免疫学活性。

Methods The target cDNA was amplified by PCR and PCR products were indigested by restriction enzyme Nco Ⅰ and Hind Ⅲ. Then the indigested products were recombined into pET-BBH and pET-BBH was expressed in E. coli BL21 (DE3) by IPTG inducing. Next, the expressed proteins were identified by SDS-PAGE, western blot and enzyme activity test. Finally, fluorescence quantitative PCR was used to test its expression quantity in different worm stages.

PCR扩增GAMMA-BBH cDNA基因，产物经NcoⅠ和HindⅢ限制性内切酶酶切后连接至原核表达载体重组为pET-BBH，在BL21(DE3)中用IPTG诱导表达，SDS-PAGE、Western blot和酶活性测定鉴定表达产物，用荧光定量PCR方法检测广州管圆线虫不同虫龄GAMMA-BBH的表达量。

When absorbed dose reached to 13.8MGy the irradiated PCS fibers become infusible fibers, and metal like black SiC fibers were obtained by heating the irradiated fibers to 1200circC in N2 gas.

当吸收剂量达到13.8MGy时，辐照产物已具有良好的不熔化特性，在N2气流中升温至1200℃热解烧成后得到有金属光泽的黑色SiC纤维，其氧含量(1.1wt％)远低于现有的热氧化不熔化法产物？

The regulation of coordination and hydrogen adsorption from non-aqueous system will show special importance to the water insolvable natural products, and will be equally effective to the water soluble products.

非水介质中的配位吸附和氢键吸附的规律对难溶于水的天然产物的吸附分离具有独特的意义，但对能溶于水的天然产物，这些规律同样适用。

The iodometric titration was used to determine the oxida- tion state of Fe ions and the oxygen nonstoichiometry in La1-xSrxFeO3-δ.

用FTIR、TG-DTA、XRD等对产物形成过程及结晶学特征进行了研究，考察了合成样品与Sm0.2Ce0.8O1.9的化学相容性；利用碘滴定法测定了该系列产物中Fe元素的平均表观化合价和氧的非化学计量值。

Solubilities were determinated for natural vitamin E and fatty acid esters under isothermal,isobaric and isochoric conditions at 40-60 ℃ and 9-17 MPa based on soybean deodorizer pretreatment,their relative separation factors were also measured.

以大豆油脱臭馏出物预处理产物为原料，在温度40—60℃、压力9—17 MPa范围内等温、等压和等密度3种条件下分别测定了预处理产物中天然维生素E和脂肪酸甲酯在超临界CO2中的溶解度，并计算了它们之间的相对分离因子。

Isocyanurate was qualitatively identified by infra-red absorption spectroscopy analysis, the quantity of isocyanurate was calculated according to mass fraction of NCO measured by di-n-butylamine method, the thermal stability of prepared elastomers was characterized by thermogravimetric analysis.

用红外光谱法证实了产物中异氰脲酸酯的存在，根据用二正丁胺法测得的NCO基团的质量分数可计算出异氰脲酸酯的质量分数，用热重法分析了产物的热稳定性。

The energy-rich intermediate anions, eg. [CH2＝C—OH]－ as the initial product of the insertion reaction and [CH2＝CHO]－ as the direct bonding product, are able to isomerize and decompose to various final products, such as C2H－＋H2O, CH2C＋OH－ and CH3－＋CO.

其中， 通过插入反应形成的富能中间体[CH2＝C—OH]－及键合中间体[CH2＝CHO]－都可以进一步经异构化和解离生成其它各种可能产物，如C2H－＋H2O， OH－＋CH2C和＋CO产物通道。

The chemical component and structure of polyborazine were analyzed, and the influence of different propylamine/isopropylamine molar ratio on the polyborazine properties and spinnability were examined.

通过分析不同正丙胺/异丙胺配比制得的聚合产物的组成与结构，探讨了不同单体的胺基取代基对先驱体的聚合反应性及对产物结构的影响。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。