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D limonene was distributed among the culture flasks (200 mg/L). The flasks were incubated at 28oC on a rotary shaker (r/min=120) for 5 days. On completion, the culture broth were successively extracted with EtOAc. The extract was quantitatively measured by gas chromatography mass spectrometry analysis, The results showed that biocatalytic reactions were comprised of oxidation, including hydroxylation and ketonization which occurred at different positions of the D limonene, as well as hydrogenation, hydrolyzation, esterification and cyclic cleavage. These bacteria strains possess the distinctive biocatalytic capacities towards the D limonene. A series of terpenoids, including sesqui , di and tri terpernoids were also observed,which was accounted for D limonene had influence on the metabolites of marine bacteria.

以2216E为培养基,添加200mg/L的柠檬烯,在28℃,以120 r/min摇瓶培养5 d,用乙酸乙酯提取培养液,经GC MS分析其转化产物,结果显示,这些细菌能在柠檬烯的不同位置进行羟基化、羰基化等,并伴随有还原、水解、酯化、开环等反应,但转化能力和转化程度不同;在产物中,还检测到系列结构不同的其它萜类:包括倍半萜、二萜以及三萜等,这些萜类化合物的产生跟柠檬烯的加入有关,说明柠檬烯能影响细菌代谢产物的产生。

The experiments of the biological activity for target molecula were accomplished. The experiment results of inhibiting activity against COX-2 indicated that the compound TM-II-5f had the highest inhibition rate, up to 95.59%, and yet the compound TM-II-5g exhibits weakly inhibiting activity.

对目标产物TM-II-5a~5g进行了生物活性研究,人环氧酶-2(COX-2)活性抑制实验结果表明:目标产物TM-II-5f对人环氧酶-2(COX-2)有很强的抑制活性,抑制率高达95.59%,目标产物TM-II-5g具有较弱的抑制率(0.61%)。

It was demonstrated thermodynamically that the initial products with respect to the uranium-hydrogen system included UH, UH2, UH3, U2H2 and U2H4. The potential energy surface analyzing results confirmed that the optimized structures and suggested, theoretically, that the main initial products were UO or linear U02 in the uranium-oxygen system, and uranium monocarbide or uranium oxycarbides in the uranium-carbon monoxide system, depending on the different interaction mode.

计算结果从热力学上证明了铀与H_2的初始反应产物可能有UH、UH_2、UH_3、U_2H_2和U_2H_4 等;随着铀与O_2相互作用的方式的不同,铀氧化的初始产物或者是UO分子,或者是直线型UO_2分子;CO在铀上的吸附属于解离吸附,并以CO的碳端与铀原子相互作用最有利,初始相互作用产物主要有碳化物和碳氧化物。

The results show that myristic acid radical anion of precursor Zn0.78 Al0.2220.22c13h27COO0.81H2O can be almost completely replaced by complex anion [La(TiW11 O39)2]^3- under special controlled reaction conditions.

结果表明,该产物中[La(TiW11O39)2](上标 13-)配阴离子沿其长轴垂直于层板的方向分布于水滑石层板间,产物有规整的层状结构、大的层间距(d003=3.29nm)、良好的结晶度和热稳定性,产物对二甘醇与苯甲酸的酯化反应、二甘醇的脱水环化反应和苯甲醛的氧化反应具有良好的催化性能。

The final products were analyzed using UV-vis absorption spectrometry and GC-MS and the occurrence of nitronaphthalene and nitronaphthol will possibly deteriorate the environmental quality.

以稳态UV-vis吸收光谱及GC-MS分析了355nm光照HNO2+萘水溶液的最终产物,其中硝基萘及硝基萘酚的产生会恶化环境质量;联系瞬态研究结果,分析了所有产物的生成途径;确定了中间产物亚硝基萘对激光闪光光解实验的影响并提出了其在355nm光照下的解离机理。

Photodissociation spectroscopy of the complexes Mg~+-NCSCH_3 Mg~+-S_2(CH_3_2 in the spectral region of 230-440nm was oberserved. As to Mg~+-NCSCH_3 of photo-induced reaction, there were three channels. And there are the products Mg~+ from nonreaction quenching and reactive products Mg~+NC, Mg~+NCS in the reaction throughout the whole wavelength range.

观察了复合物Mg~+-NCSCH_3Mg~+-S_2(CH_3_2在230~440nm范围的光解离光谱,在此波段内的复合物光诱导产物的质谱显示,对Mg~+-NCSCH_3:光诱导反应有三个通道,存在着非反应猝灭产物Mg~+和反应产物Mg~+NC、Mg~+NCS。

Mass spectra of the products of photo-induced reaction of the complexes Mg(superscript +)-NCSCH3 and Ca(superscript +)-NCSCH3 exhibits the presence of Mg(superscript +)Ca(superscript + from nonreaction quenching, Mg(superscript +)Ca(superscript +NC and MgCa(superscript +NCS from the rupture of S-C bonds, and rearrangement products Mg(superscript +) Ca(superscript +-CHSH.

复合物Mg-NCSCH3,Ca-NCSCH3光诱导反应的产物质谱表明有非反应猝灭产物MgCa(上标+,C-S键断裂产物Mg Ca(上标+NC和MgCa(上标+-NCS以及重排反应产物MgCa(上标+-CHSH通道。

The compoud 1 can be converted to intramolecular coupled cyclic olefine (2) as a main product in the presence of iron catalyst.

控制反应条件,在铁催化下可以生成偶联烯烃化的环烯产物(2 )为主要产物;不加催化剂,加热回流反应得到分子内亲核取代反应产物环丙烷化合物 3

The most of the product became soluble from inclusion body by lowering induction temperature. At the same time the gene was cloned into vector pMAL-p2X, which was translated in E. coli TB1 and expressed a fusion protein. We optimized the induction time and achieved high expression. But there was no obvious product secreted in the periplasm extract. Almost all fusion protein is expressed soluble in cytoplasm.

利用融合型表达载体pTYB11-EFE融合型表达,对诱导剂IPTG的浓度和诱导温度进行了优化,通过降低诱导温度使产物由包含体变成可溶性融合型产物;利用融合型分泌表达载体pMAL-p2X构建了表达载体pMAL-p2X-EFE-7~#,对诱导时间进行了优化,获得了较高的表达量,但发现产物没有明显的分泌表达,绝大部分是以可溶形式存在于胞内。

The short time oxidized product is insoluble in common organic solvents and water, but the water-soluble derivatives were obtained after hydrolysis under a basic condition. It′s IR spectrum is similar with that of C60 polyhydric derivatives. The extensive oxidation product of C60 is water-soluble without any further dispose.

一种产物在有机溶剂及水中均不溶,但经碱性水解后得到水溶性产物,其红外光谱与C60羟基化合物相似;在长时间通O3条件下,得到水溶性的C60氧化产物

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