产分生孢子的

In this article,the type of Alternaria separated from pear of Hebei and Xinjiang of China,and USA,Japan and Chile was studied on sporulation patterns,conidia characteristics and molecular biology.

本文从链格孢的产孢表型、分生孢子特征和分子生物学方面研究了从河北鸭梨、新疆库尔勒香梨、美国和日本以及智利的梨上分离的链格孢菌的种类。

The optimum pH is 8-10 for hypha growth and 7-9 for conidiospore germination; The hypha could not grow on the culture medium which nitrogen source comes from urea and asparagines, but it could grow fast and produce more conidiospore on the culture medium which carbon source comes from maltose, glucose and lactose, and nitro0gen source come from glycine, histidiane and peptone.

该菌在PH 4~12范围内均能生长、产生分生孢子并萌发，菌丝生长的最适pH 8~10。，孢子萌发的最适PH 7~9；该菌不能在以尿素和天门冬酰氨为氮源的培养基上生长，但以麦芽糖、葡萄糖、乳糖、淀粉、甘氨酸、组氨酸和蛋白陈为碳源和氮源时，菌丝生长快、产孢量大；光照处理对该菌菌丝生长、产孢和孢子萌发无显著影响。

The test established optimal conditions for producing pycnidium that optimal medium was corn powder,optimal temperature was 25-30℃,optimal pH was 7-8.UV irradiation induced the forming of pycnidium.

最适合草茎点霉SYAU-06菌株形成分生孢子器的培养基为玉米粉培养基；最适温度为25-30℃，最适pH为7-8，而且光照有利于产孢，适量的紫外线照射可诱导分生孢子器的形成。

As a result, some valuable taxonomicmethods, criteria and our viewpoints were concluded. The morphology ofthe sporus is the main identical base at species level, which include thecharacters of the shape, size, colour, septate and ornament of the wall etc,and some sporus with distinct variable are also useful at genus level.

孢子的特征(包括形状、大小、颜色、隔膜数和表面纹饰等)是种级分类的主要依据，一些形态差异较大的孢子可作为属的划分标准；其次，分生孢子梗的形态特征、产孢细胞的延伸方式及产孢方式也是主要的鉴别依据，对属级水平上该真菌的鉴别有重要意义；另外，培养特征及产孢表型可作为分类鉴定的必要、有益补充。

The citric acid-producing strain Aspergillus niger 2363-2, was isolated and used in our research. The fermentation conditions were optimized as follows: sucrose as carbon source 8. 0-14. 0%, ammonium chloride as nitrogen source 3. 0-4. 0g/l, initial pH 2. 0, and cultural temperature 30℃. The influential factors of formation of spores were also studied, and a series of measures were taken to restrain the spore formation on the basis of our experimental results. With the consideration of the cultural environment and microbial cell itself, the factors affecting the duration of citric acid-producing activity of microbial cells were examined, and it is found that undissociated citric acid and the ageing of microbial cells are the main factors. The experimental results also show that partial replacement of fermentation broth can lengthen the duration of citric acid -producing acitivity of microbial cells.

筛选出了用于试验研究的柠檬酸发酵用菌Aspergillus niger 2363-2，确定了其最适发酵条件—碳源选用蔗糖，浓度8.0~14.0%，氮源为NH〓Cl，浓度3.0~4.0g/l，发酵初始pH值为2.0，培养温度30℃；讨论了分生孢子产生的影响因素，并通过试验提出了抑制分生孢子产生的措施；从分析微生物所处的环境和微生物本身两个方面出发，探讨了影响微生物菌体产酸活性持久性的因素，认为分子态柠檬酸和菌体本身的老化是影响微生物产酸活性持久性的两个主要因素，并提出采用部分置换发酵液发酵能延长微生物菌体的产酸活性。

The results showed that resistant gene Ht in F1 heterozygote was the most effective on controlling spots number and size; all monogene in heterozygote could produce spore by moisture culture and resistant gene Ht1 was the most effective; resistant gene Ht1 in medium level, Ht3 in high level, and Ht in low level of vertical resistance of heterozygote, were the most effective on controlling spots spread; while Ht2 in high level of vertical resistance of heterozygote was the most ineffective on controlling spots spread; and all resistant monogene in heterozygote showed superior to their parents on controlling incubation period and gene Ht was more significant than other genes.

结果表明，抗性单基因Ht处于F1代杂合状态时，控制病斑数和病斑面积的效应最强；单基因处于杂合状态时，湿培48h病斑全部能产生分生孢子，且处于同一水平抗性杂合状态时，Ht1控制产孢量的效应最强；Ht1处于中水平、Ht3处于高水平和Ht、处于低水平垂直抗性杂合状态下，控制病斑扩展效应最强；而Ht2处于高水平垂直抗性杂合状态下，控制病斑扩展效应最弱；抗性单基因处于杂合状态下，控制潜伏期多表现超亲现象，Ht控制病害潜伏期比其它单基因显著。

Growth and sporulation conditions of Alternatia citri were studied in the experiment. The results showed that mannose was the best carbon source and valine was the best nitrogen source. Czapek medium was the most appropriate for growth,and citrus leaf medium was the most appropriate for sporulating.

本试验研究了柑桔黑腐病菌Alternaria citri菌丝生长和分生孢子形成条件，结果表明：病菌在27℃菌丝生长最快，在30℃产孢最多；甘露糖是最好的碳源，D-缬氨酸是最好的氮源，Czapek培养基是菌丝生长最适宜的培养基，桔叶汁培养基是产孢的最适宜培养基。

It"s vegetal mycelia were colorless, smooth, 1.2μm～3.0μm crude, manyconidiogenous cells born on vegetal mycelia, sometimes as many as a dozen,a very small number were single. The base of conidiogenous cell wasenlarged and many of them were bottle-shaped or spherical. The upper ofconidiogenous cell extended into a conidiogenous axis of 1μm wide and 14μm length which was the knee-bending ("Z like bending), there were small pectinae on the axis, conidium located on the small pectina.

营养菌丝无色，光滑，有隔，粗1.0μm～3.0μm，产孢细胞常浓密簇生于菌丝或泡囊上，有时可多达十几个，极少数单生，产孢细胞基部膨大，多为瓶形，近球形，颈部明显延长成粗1μm，最长达14μm的产孢轴，呈膝状弯曲，轴上具小齿突，分生孢子位于小齿突上。

So the efficiency of topsin-M in the lab was the best among the four fungicides, the effects of carbendazim and chiororthalonil were good,too.So they could be also used to control corn ear rot in practice.

另外，多菌灵和百菌清对病菌生长、产孢和分生孢子萌发的抑制效果也比较良好，都可以在生产中用来防治玉米穗腐病。

Additionally, the growth of the pathogen was obviously affected by cultural media. The morphological characters such as colony color and diameter, and production of fruit body of the fungus growing on PDA, 10% V8, apple leaves dextrose agar and apple leaves extraction dextrose agar media were different. The fungus formed colonies of about 8mm in diameter and did not produce fruit bodies and aerial hyphae in 1 month incubation on PDA. However it formed the similar size colonies as on PDA and produced fruit bodies and aerial hyphae on 10% V8 and LDA media in the same incubation period. Very small colonies (2mm in diameter) and fruit bodies were found on LEDA media in the same incubation condition. These results indicated that successful isolation of M. coronaria from apple leaves depended on suitable isolation method and cultural media as well as fresh samples.

不同培养基上菌落形态、大小和产孢情况差异也很大，培养1个月（25℃）后PDA上菌落黑褐色隆起，表面蚯蚓粪状，无气生菌丝，无子实体和基内菌丝；10% V8培养基上菌落中央隆起，黑褐色，表面生少量气生菌丝，边缘放射状，基内菌丝深褐色，有子实体；苹果叶片葡萄糖琼脂培养基上菌落平坦，黄褐色，表面生茂密的金黄色气生菌丝，基内菌丝深褐色，有子实体；苹果叶片煎汁葡萄糖琼脂培养基上菌落有明显的不规则隆起，黄褐色至黑褐色，表面生少许气生菌丝，菌落生长缓慢，无基内菌丝，分生孢子盘菌落表面生，菌落直径仅2mm左右，而在其他培养基上的菌落直径可达6－8mm，说明培养基质、分离方法均对苹果盘二孢的分离培养和生长发育有明显的影响。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐