交叉杂交

If you have your heart set on one of the new crossbreeds; try to learn what you can before deciding on the particular dog or cross.

如果您有您的心脏上设置一个新的杂交；尝试了解什么，您可以，然后才决定对特定的狗或交叉。

If you have your heart set on one of the new crossbreeds; try to learn what you can before deciding on the particular dog or cross.

如果您有您的心脏上设置一个新的杂交；尝试了解什麼，您可以，然后才决定对特定的狗或交叉。

A competitive ELISA kit for detect ENR was developed with ENR mAb and its traits were tested.Two hybridoma lines were filtered and the best one was 4G1-B3,which secreted ENR mAb with indirect ELISA titers of 1∶1.024×10~6 in ascites.Isotype of the two mAb was IgG_1.ENR mAb had a high affinity constant with 9×10~(10)L/mol.The ENR-Kit had the linear detection range of 0.05～101.6μg/L,the sensitivity of 0.05 μg/L and a good sensitivity with an IC_(50) of 1.1μg/L to ENR,cross-reactivity to other compounds less than 0.01%.The average recoveres of ENR spiked in chicken,liver,fish and milk were 81.5%,87.6%,84.3% and 95% respectively.The coefficient variation was below 10%.

结果表明：筛选出的2株杂交瘤细胞，单抗亚类均为IgG1型，其中4G1-B3株的ENR mAb间接ELISA效价为1：1.024×106，亲和常数为9×1010L/mol；竞争ELISA试剂盒的线性检测范围为0.05~101.6μg/L、灵敏度0.05μg/L、半数抑制浓度(IC50)1.1μg/L，与其他化合物的交叉反应率均小于0.01%，鸡肉样、鸡肝样、鱼肉样和牛奶样的平均添加回收率分别为81.5%8、7.6%、84.3%和95%，不同基质添加恩诺沙星标准品做竞争ELISA对ENR-Kit检测结果影响小，试剂盒保存期6个月以上。

Results 371 bp DNA fragment was amplified from 24 different species. The sensitivity could be improved to 10-12 g. No signal was observed when human DNA,funguses and viruses were used as templates. 22 blood samples and 4 cerebrospinal fluid samples, being positive with culture, were positive by using PCR. The gramnegative and grampositive probes hybridized to clinic samples and different species, as predicted by Gram stain characteristics.

结果 对24株不同标准菌株进行PCR扩增，均出现371 bp长度的DNA片段，敏感性试验可检测出10-12 g的细菌DNA，与人类基因组DNA、真菌及病毒无交叉反应；22例血培养阳性标本及4例脑脊液培养阳性标本均扩增出371 bp长度DNA条带，反相杂交法区分革兰阳性/阴性细菌与培养结果相符。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。