亡

METHODS: After AIF△1-120 gene was successfully cloned, the shorter truncated human AIF gene (AIF△1-400) was constructed by deleting the Nterminal mitochondrial localization sequence, the coding sequence of flavine adenine dinucleotide binding domain and nicotinamide adenine dinucleotide binding domain of AIF protein.

在AIF△1-120基因克隆成功的基础上进一步改造，构建截去AIF基因线粒体定位信号，FAD结合结构域和NADH结合结构域（1-400位氨基酸）编码序列的AIF△1-400基因，将其克隆入pcDNA3和pIRES2EGFP真核表达载体，用脂质体法转染HeLa细胞，通过荧光显微镜观察，MTT检测等方法检测该基因在转染细胞中的表达及其对肿瘤细胞的促凋亡活性。

Poptosis-inducing factor is a mitochondrion-localized flavoprotein with NADH oxidoreductase activity, which is encoded by the nuclear gene. Following induction of apoptosis, AIF is released from mitochondria and translocated to cytosol, and then to the nucleus. The mechanism of AIF translocation from mitochondria to nucleus is still unclear. It has been suggested that translocation is due to the presence of nuclear localization signal.

bstract： AIF(apoptosis-inducing factor)为位在粒线体内膜膜上之蛋白质，在正常细胞中，能够藉由维持电子传递链复合体I的稳定性，进而影响细胞能量的产生及自由基的清除，并且也会维持粒线体的构造；反之，当细胞遭受到不当刺激时，粒线体膜通透性改变，使得原本存在於粒腺体间质中之AIF，被释放出来，进入细胞核内造成DNA片段化，进一步促进细胞凋亡。

We next demonstrated that OSU-03012 induced reactive oxygen species generation by using H2DCFDA-based flow cytometry and florescence microscopy detection.

大量的ROS可以通过caspase的活化导致凋亡性细胞死亡，但是在某些特定的生理环境下，ROS亦可以在不同的肿瘤细胞中导致自噬性细胞死亡。

The double flourescent staining of apoptosis and expression of transforming growth factor β1 (TGF β1) were examined in liver tissue of animal pattern of ADR-injury by immunohistochemical and TUNEL,and the level of lipid peroxidation in serum was observed.

结果 阿霉素组大鼠肝组织可见细胞凋亡和肝细胞中转化生长因子β1的异常表达；阿霉素组大鼠血清中脂质过氧化物明显升高；亚硒酸钠对阿霉素的损伤有明显的保护作用。

Methods The double flourescent staining of apoptosis and Desmin was examined in heart tissue of Keshan disease by immunohistochemical and TUNEL,and the confocal microscope was used to observe the results.

应用原位末端标记法及免疫组化技术在亚急型和慢型克山病患者心肌石蜡包埋组织切片上进行细胞凋亡及Desmin双重荧光染色，并应用共聚焦显微镜观察结果。

Methods Using Wistar rats as animal pattem of ADR-induced myocardial injury and sodium selenite as protective agent.The double flourescent staining of apoptosis and expression of transforming growth factor β1 (TGF β1) were examined in liver tissue of animal pattern of ADR-injury by immunohistochemical and TUNEL,and the level of lipid peroxidation in serum was observed.

通过复制大鼠阿霉素性心肌损伤的动物模型，以亚硒酸钠作为保护因素，应用原位末端标记法和免疫组化技术检测大鼠肝细胞凋亡和肝细胞转化生长因子β1表达，并观察了大鼠血清中脂质过氧化含量。

At 18 and 24 hours after culture, types of cell death were quantitatively detected using fluorescein isothiocyanate and propidium iodide staining.

25%，50%，100%浓度的3种材料浸提液体外培养小鼠成纤维细胞L929 24 h后，采用流式细胞术碘化丙啶染色法检测L929细胞的凋亡率及细胞周期分布情况。

Objective: To evaluate the usage of fluorescent microscope in analyzing cellular apoptosis.

目的 ：探讨荧光显微技术在检测细胞凋亡中的作用。

Objective To observe the effects of fluoride on spermatogenic cell apoptosis in adult male rats.

目的 观察氟对成年大鼠生精细胞凋亡的影响。

Objective To investigate the effects of selenium and fluoride on the cell cycle and apoptosis of human hepatocytes in vitro.

目的 研究硒、氟对离体人肝细胞周期和凋亡的影响。

We have no common name for a mime of Sophron or Xenarchus and a Socratic Conversation; and we should still be without one even if the imitation in the two instances were in trimeters or elegiacs or some other kind of verse--though it is the way with people to tack on 'poet' to the name of a metre, and talk of elegiac-poets and epic-poets, thinking that they call them poets not by reason of the imitative nature of their work, but indiscriminately by reason of the metre they write in.

索夫农 、森那库斯和苏格拉底式的对话采用的模仿没有一个公共的名称；三音步诗、挽歌体或其他类型的诗的模仿也没有——人们把&诗人&这一名词和格律名称结合到一起，称之为挽歌体诗人或者史诗诗人，他们被称为诗人，似乎只是因为遵守格律写作，而非他们作品的模仿本质。

The relationship between communicative competence and grammar teaching should be that of the ends and the means.

交际能力和语法的关系应该是目标与途径的关系。