亡

Contents include cell culture 、 cell fusion 、 Cytochemistry 、 apoptosis detection 、 protein expression and function determination , etc.

课程涉及的主要实验内容包括细胞培养、细胞融合、细胞化学、细胞凋亡检测和蛋白质的表达与功能检测等。

ObjectiveTo evaluate the effects of caspase-3 activation and cytochrome c release in cerebral ischemia reperfusion induced neuronal cell death.

目的探讨Caspase-3激活和细胞色素c释放在脑缺血再灌注损伤后神经元细胞凋亡中的作用。

The results showed that after treatment of neural stem cells by RA, apoptosis factor cytochrome c was released.

同时应用Western blot检测RA诱导神经干细胞凋亡过程中细胞色素C的变化，结果发现在细胞质中有细胞色素C的释放。

In the process of conversion from androgen to estrogen, cytochrome P450 aromatase plays an important role.

在一些动物实验中发现，芳香化酶以及性激素能够引起某些凋亡因子的变化。

Methods: Introduce cytopathology experimental methods, human hepatic cancer cell line Bel-7402 co-cultured with Triptolide (10 g/mL, 5μg/mL and 0.25μg/mL), Using MTT method to test the effect of Triptolide on Bel-7402 cell proliferation, using TUNEL technique to test the effect of Triptolide on Bell-7402 cell apoptosis, immunohistochemistry staining method to test Bax and Bcl-2 protein expression.

采用细胞药理学实验方法，人肝癌细胞株Bel-7402与雷公藤甲素（10μg/mL、5μg/mL及0.25μg/mL）共培养，MTT法检测其对Bel-7402细胞增殖的影响，TUNEL技术检测其对Bel-7402细胞凋亡的影响，免疫组织化学染色法检测Bax及Bcl-2蛋白表达。

Conculsion hTERT RNAi may suppress hTERT expression to result in reduction of Bcl-2 and increase of Bax, and then induce hepatocarcinoma HepG2 cell apoptosis by mitochondrial pathway subsequent to cyt C release from mitochondria to cytoplast.

RNAi靶向抑制hTERT基因可通过抑制Bcl-2表达和促进Bax表达引起线粒体cyt C释入胞质，激活caspase-9促进HepG2肝癌细胞凋亡。

Objective To investigate the effects of cell cycle and apoptosis by photodynamic therapy on human colon carcinoma cell transfected with cytosine deaminase gene.

目的：观察PDT对SW480细胞和转染CD自杀基因的SW480结肠癌细胞周期和凋亡的影响。

Objective: To investigate the expression of Cytotoxin associated gene A and Vacuolating cytotoxin A,gastric epithelial cell proliferation and apoptosis of chronic superficial gastritis with helicobacter pylori infection and Spleen - Stomach Damp-Heat .

目的：观察幽门螺杆菌感染慢性浅表性胃炎脾胃湿热证患者血清中CagA、VacA的表达及胃粘膜上皮细胞增殖和细胞凋亡情况。

Results 2 ClAdo and 2 CldAdo had different cytotoxicities on the cells, while 2' dAdo had no cytotoxicity on the cells. 2 CldAdo blocked the cell growth at the phase of S and G2/M, while 2 ClAdo at the phase of G0/G1. 2 CldAdo mainly induced cell necrosis, while 2 ClAdo induced both cell necrosis and apoptosis. Down regulated Bcl 2 expression and up regulated p53 and Bax expression were associated with 2 ClAdo, while 2 CldAdo down regulated Bcl 2 expression and up regulated mildly p53 expression without any effect on Bax expression.

结果 2 ClAdo、2 CldAdo对所研究的细胞均有程度不等的毒性；2' dAdo对各细胞生长、增殖均无明显抑制（c=100 μmol/L即30 μg/mL内）；2 CldAdo明显阻断细胞于S、G2/M期，2 ClAdo阻断细胞于G0/G1期；2 CldAdo 致细胞坏死为主，2 ClAdo 既致细胞坏死又诱导凋亡；2 ClAdo 下调Bcl 2，上调P53、Bax蛋白表达；2 CldAdo小幅上调P53、小幅下调Bcl 2，不影响Bax蛋白表达。

I traveled nights, and hid daytimes and slept, and the bag of bread and meat I carried from home lasted me all the way, and I had a-plenty.

随后我告诉她，我父母双亡，按照法律，把我给栓住在乡下一个卑鄙的农民手里，离大河有三十英里。

We have no common name for a mime of Sophron or Xenarchus and a Socratic Conversation; and we should still be without one even if the imitation in the two instances were in trimeters or elegiacs or some other kind of verse--though it is the way with people to tack on 'poet' to the name of a metre, and talk of elegiac-poets and epic-poets, thinking that they call them poets not by reason of the imitative nature of their work, but indiscriminately by reason of the metre they write in.

索夫农 、森那库斯和苏格拉底式的对话采用的模仿没有一个公共的名称；三音步诗、挽歌体或其他类型的诗的模仿也没有——人们把&诗人&这一名词和格律名称结合到一起，称之为挽歌体诗人或者史诗诗人，他们被称为诗人，似乎只是因为遵守格律写作，而非他们作品的模仿本质。

The relationship between communicative competence and grammar teaching should be that of the ends and the means.

交际能力和语法的关系应该是目标与途径的关系。