亡

L^(-1) VP-16 for 6, 12, 24 and 48 h. Meanwhile or 24 h later, 316 mg?L^(-1) SOD was added to the culture to act for 24 h, and then, the intracellular reactive oxygen species, apoptosis ratio and expressions of mutant p53 and c-fos protein of Hela cells were determined. Results The production of ROS and the apoptosis of Hella cells increased as the action of DDP and ADM continued within 24 h, hut VP-16 failed to produce such effects.

宫颈癌Hela细胞经顺铂、足叶乙甙(VP-16)和阿霉素血浆峰浓度不同时间作用后，测定肿瘤细胞内活性氧水平、细胞凋亡率及突变型p53和c-fos基因表达的变化，并测定不同时间加入SOD对DDP、ADM、VP-16诱导Hela细胞凋亡及p53、c-fos基因表达的影响。

EPO can protect the ischemic retina in part, and it is helpful to recover the function of retina.

在视网膜缺血再灌注损伤中，存在有视网膜细胞的凋亡，EPO能够减少视网膜细胞的凋亡。

Objective: It is hotspot to study the effect of diagnostic ultrasound on apoptosis and ultrastructure in special tissue.

目的 应用彩色多普勒超声照射后组织细胞凋亡以及超微结构的变化，是目前超声安全性研究的热点，但是凋亡和超微结构的变化是否会影响子代的脑功能，目前的研究仍没有确切的结论。

At 3 h, 6 h, 12 h after operation, the L 4～L 5 spinal cord cells of the two groups were examined by terminal transferase and bioti...

结果表明，TUNEL显示生理盐水组在三个时间段均出现大量脊髓细胞凋亡，而 NGF组的凋亡细胞数量很少。

Hypoxia was imposed to the cardiomyocytes with or without daidzin pretreatment. ALDH2 activity was measured by the method of acetaldehyde metabolism. Apoptosis was measured by Hoechest 33324 staining, fluorescence activated cell soiling and the DeadEnd fluorometric TUNEL system.

运用缺氧模型，比较大鼠心肌细胞在单独缺氧和经ALDH2特异性抑制剂daidzin预处理24h后缺氧的凋亡改变，酶活性检测采用乙醛代谢法、凋亡的测定通过用Hoechest 33324、免疫荧光标记用流式细胞仪测定和TUNEL试剂盒检测。

Objective To investigate the relationship between apoptosis of peripheral blood lymphocytes and impaired immunity by assessing the apoptosis and expression of Fas and Bcl-2 in PBLCs of patients with secondary syphilis.

目的检测二期梅毒患者外周血淋巴细胞凋亡及其Fas、Bcl-2的表达，探讨二期梅毒患者免疫功能异常与淋巴细胞凋亡的关系。

Dysfunction of cellular immunity may be due to the increased PBLC apoptosis in patients with secondary syphilis, which is associated with the abnormal expression of Fas and Bcl-2 on PBLCs.

结论二期梅毒患者细胞免疫功能异常可能与淋巴细胞凋亡过度有关，而淋巴细胞凋亡过度与Fas、Bcl-2表达异常有关。

Nucleus changes in apoptotic cells were investigated by using Hoechst 33258 staining and photofluorography. Apoptotic rate was evaluated by propidium iodide staining and flow cytometer.

应用Hoechst 33258染色法观察凋亡细胞的形态学改变；PI染色流式细胞仪检测细胞凋亡率；Western印迹法检测Bcl-2蛋白的表达。

CoCl2 (a chemical hypoxia-mimetic agent) was used to establish the chemical hypoxia-induced PC12 cell injuries model. Sodium hydrosulfide was used as a H2S donor. The viability of PC12 cells was measured by CCK-8 assay. The percentage of apoptotic cells was assessed by propidium iodide stain flow cytometry. The morphological change of apoptotic cells was tested by using the chromatin dye Hoechst 33258. The mitochondrial membrane potential was analyzed by rhodamine 123 staining and photofluorography. The level of reactive oxygen species in PC12 cells was measured by DCFH-DA staining and photofluorography.

应用化学性缺氧模拟剂CoCl2在PC12细胞建立化学性缺氧损伤模型；以硫氢化钠作为H2S的供体；应用CCK-8比色法检测细胞存活率；碘化丙啶染色流式细胞技术检测细胞凋亡率；Hoechst33258染色检测细胞凋亡的形态学变化；罗丹明123(Rh123)染色荧光显微镜照相检测细胞线粒体膜电位；2'，7'-二氯荧光黄双乙酸盐染色荧光显微镜照相检测细胞内的活性氧水平。

AIF ex- pression in ischemia region of cortex of parietal lobe and nerve cell apoptosis degree in sham operation group, MCAO 90 min and reperfusion 0, 3, 6, 12, 24, 48 and 72 h were observed by TUNEL and immunohistochemistry technique.

除缺血90min凋亡细胞数量与假手术组比较无统计学意义外，其余各指标与假手术组相比均有统计学意义（P〈0.01），除6与12h以及24与48hAIF含量的差异无统计学意义，24与48h凋亡细胞数量的差异无统计学意义外，其余各组间差异均有统计学意义（P〈0.05）。

The labia have now been sutured together almost completely.The drains and the Foley catheter come out at the top.

此刻阴唇已经几乎完全的缝在一起了，排除多余淤血体液的管子和Foley导管从顶端冒出来。

To get the business done, I suggest we split the difference in price.

为了做成这笔生意，我建议我们在价格上大家各让一半。