亡

In the Mainland of multi-fingered to store the cash, be done quickly.

在边陲多指现金的保不亡所，需用时怠工到飞迟索不亡。

The inhibition of SPG-Rg3 on the growth of MCF-7 cells was detected by MTT assay,IC50 was calculated to obtain the effective concentration; flow cytometry was used to observe the cell cycle of MCF-7 cells; AO/EB fluorescence double-dye technology was performed to observe the apoptosis of cells in morphology,immunocellularchemistry and RT-PCR were utilized to investigate the apoptosis of MCF-7 cells and its relationship with caspase-8 from protein level and molecule level.

采用MTT法观察SPG-Rg3对MCF-7细胞生长的抑制作用，并计算出IC50，依据IC50值确定SPG-Rg3的有效浓度；流式细胞术检测SPG-Rg3作用后MCF-7细胞周期的变化；AO/EB双染从形态学上观察SPG-Rg3对MCF-7细胞凋亡的作用；免疫细胞化学染色和RT-PCR技术分别从蛋白水平和分子水平上检测SPG-Rg3对MCF-7细胞的诱导凋亡作用及其与caspase-8基因的关系。

Objective To investigate the efficacy of inducing apoptosis in human bladder cancer cells by adriamycin and mitomycin and relevant mechanism.

目的探讨化疗药物诱导膀胱癌细胞凋亡及其对X染色体连锁的凋亡抑制蛋白表达和caspase-3剪切的影响。

Result:The apoptosis has been observed within the hair follicle cells and apocrine gland cells after irradiation.

结果 ：半导体激光照射后毛囊上皮细胞及大汗腺腺体细胞中出现凋亡细胞；低剂量重复治疗凋亡现象增加。

This study was purposed to observe the changes of caspase-3 activity during apoptosis of HL-60 cells induced by an iron chelator, DFO, and to explore the mechanism underlying apoptosis in HL-60 cells.

为了研究铁螯合剂—去铁胺(deferoxamine，DFO)诱导人白血病细胞株HL-60凋亡时半胱天冬酶-3(caspase-3)活性的变化并探讨DFO诱导凋亡的可能机制。

Vitrectomy allows us to manage otherwise untreatable ocular pathologies, but the substitution of the vitreous by an artificial intraocular irrigation solution would probably disturb the physiology of the retina and choroids.

中文题名眼内灌注液对视网膜色素上皮细胞凋亡的影响副题名外文题名 The effects of different intraocular irrigating solutions on the apoptosis of RPE cells 论文作者殷春悦导师黎晓新教授学科专业眼科学研究领域\研究方向临床医疗技能训练与研究学位级别博士学位授予单位北京大学学位授予日期2001 论文页码总数94页关键词玻璃体切除术眼科手术细胞凋亡视网膜色素上皮细胞眼内灌注液馆藏号BSLW /2001 /R779.6 /42 随着玻璃体切除术的出现和日益发展，眼内灌注液作为术中使用的玻璃体替代物，直接影响视网膜和脉络膜的正常生理功能。

Results After cocultured with rhG-CSF (10?ng/ml) for 96 hours, the apoptosis percentage of Fas transferred HL-60 cells was significantly higher than that of HL-60 cells, and the Fas expressions could be up-regulated by rhG-CSF.

结果 相同浓度的rhG-CSF作用相同时间后转染的HL-60细胞凋亡率较未转染的HL-60细胞凋亡率明显增加，且随rhG-CSF作用时间延长，Fas在HL-60细胞中的表达逐渐增加。

Cerebral cortexes were removed and then dissociated, measured with flow cytometer analyze cell apoptosis, the morphological changes were examined by electron microscope.(2) Measured cell apoptosis of cerebral cortexes with TUNEL.(3) detected expressions of Bcl-2, Bax and Caspase-3 proteins with the technique of immunohistochemistry.

1取大脑皮层细胞制成细胞悬液，流式细胞仪分析细胞凋亡改变，电镜观察细胞超微结构的变化；(2)用原位缺口末端标记法检测凋亡细胞百分率；(3)免疫组化法检测Bcl-2、Bax和Caspase-3蛋白表达。

METHODS: Firstly,depanding on the methods of FCM,MTT etc,we got the best density and actiontime of adriamycin.and then,we used several depressors of different signal transduction pathways to observe the possible pathway of osteosarcoma cell apoptosis.

首先通过FCM，MTT等方法探讨引起成骨肉瘤细胞凋亡的最佳药物作用浓度及时间；在此条件下应用不同信号转导通路的抑制剂观察导致细胞凋亡的可能通路；最后应用Western？？？？

The -300V and -500V electrets were applied on 3T3 cells for 24 and 48h, respectively, then the surface charge density on 3T3 cells and the intracellular free Ca2+ concentration and the number of apoptosis were measured by means of cell electrophoresis and flow cytometry method, respectively.

利用Fluo—3/AM Ca2+指示剂，采用流式细胞术测定3T3细胞内钙离子浓度的变化和3T3细胞的凋亡量，探讨驻极体诱导3T3细胞凋亡与细胞表面电荷密度和游离钙浓度变化之间的关系。

The labia have now been sutured together almost completely.The drains and the Foley catheter come out at the top.

此刻阴唇已经几乎完全的缝在一起了，排除多余淤血体液的管子和Foley导管从顶端冒出来。

To get the business done, I suggest we split the difference in price.

为了做成这笔生意，我建议我们在价格上大家各让一半。