亡

Aim: To investigate the efficacy on growth suppression and apoptosis of undifferential NPC cell line CNE-2 treated by Paclitaxel and the expression of Survivin in this course.

目的：探讨紫杉醇对低分化的鼻咽癌细胞株CNE-2生长抑制率、凋亡的影响以及凋亡抑制基因Survivin的变化。

Furthermore, preliminary work also performed to examine whether PI3K/AKT signal transduction pathway was activated in the process of refractory leukemia development. Materials and methods An immortalized human bone marrow stromal cell line, HS-5, was introduced to establish a bi-phase culture system for the cultivation of B-lineage precursor leukemia cells. ELISA and RT-PCR were used to investigate the expression of VEGF and its receptors in the leukemia cell lines and primary childhood leukemia cells in different treated groups. Flow cytometory method and immunofluorescent staining were employed to examine the apoptosis signals both in the VP16 treated and untreated leukemia cells. Western blot was utilized to explore the PI3K/AKT activated status in the drug induced or uninduced leukemia cells and lymphocytes from healthy donors.

材料和方法使用来源于人类骨髓基质细胞的细胞株HS-5作为滋养层细胞进行急性淋巴细胞性白血病细胞的体外培养，通过细胞生物学和免疫学方法评估培养体系并鉴定出难治性白血病细胞克隆；以ELISA和RT-PCR方法检测急性白血病细胞株和患儿白血病细胞VEGF及其受体的表达，了解不同治疗阶段VEGF及其受体的表达状况，并结合临床指标进行分析，明确VEGF及其受体在白血病发生过程中的作用；流式细胞仪和免疫荧光染色法对正常健康儿童、初发白血病患儿、复发白血病患儿及缓解后患儿进行凋亡因子检测和分析，初步阐明难治性白血病抗凋亡形成的原因；蛋白印记分析检测PI3K/AKT信号传导通路在健康儿童、初发白血病和复发白血病患儿的表达，初步了解难治性白血病形成的分子生物学机制。

Massion stain showed the collagen of the lesion was less in the early pregnant group with acute myocardial infarction than that in unpregnant group with acute myocardial infarction.

两组凋亡指数比较早孕心梗组凋亡指数明显高于未孕心梗组。massion染色显示：早孕心梗组心肌胶元含量明显较未孕心梗组少。

AIM: To study the effects of ursolic acid on human MCF7 cell apoptosis and the mechanism probably involved.

目的：探讨中药成分熊果酸（ursolic acid， UA）诱导MCF7人乳腺癌细胞的凋亡作用，并通过UA作用后的MCF7细胞Bax， Bcl2， cytochrome c蛋白表达的变化，探讨其诱导MCF7细胞凋亡的分子机制。

Results: Compared with that treated with anti-thyroid drugs alone, after the combined therapy, the size of thyroid decreased significantly, P＜0.01. Typical apoptosis appearance, such as vacuolated cell, marginal nuclei, chromatin condensation and nuclear fragmentation, could be seen under light microscopy. The apoptotic rate before and after adding Chinese herbs was (2.11±1.78)%and (18.66±20.01)%respectively (P＜0.01). TUNEL positive cells were detectable for up to 2-20 weeks after Chinese herbal treatment.

结果：与单纯抗甲状腺药物治疗比较，患者经抗甲状腺药物与中药联合应用治疗2-10周后，甲状腺组织明显缩小(P＜0.01)；光镜下可见细胞发泡、核着边、核固缩、核小体、核分裂等凋亡细胞的典型形态；加中药治疗前后甲状腺细胞凋亡比率分别为(2.11±1.78)%和(18.66±20.01)%(P＜0.01)；末端标记呈阳性。

Methods Model of vasoligation and vasostomy in male rat was established, an d then terminal deoxynucleotidyl transferase-mediated dUTP nick labelling techn ique to detect the apoptosis of spermatogenic cells at 4, 8, 12, 16 weeks after v asostomy.

以雄性SD大鼠为研究对象，建立输精管阻断及阻断后复通模型，采用DNA缺口原位标记凋亡细胞方法，观察大鼠生精细胞在输精管复通后4、8、12及16周的凋亡状况。

In this study, human tongue cancer Tca8113 cells and human umbilical venular endothelial cell ECV304 are treated with CPT and the cell proliferation and apoptosis are detected in order to investigate the effect of CPT on Tca8113 and the mechanism of antiangiogenesis.

本研究以喜树碱作用于舌鳞癌细胞(Tca8113)及人脐静脉血管内皮细胞(ECV304)，检测细胞增殖及凋亡状况，以研究喜树碱对舌鳞癌细胞及血管内皮细胞的生长抑制和凋亡诱导作用，探讨喜树碱抗鳞癌的作用及可能机理，并初步探索其抗血管生成机制，为该药治疗皮肤肿瘤和银屑病奠定实验基础。

ACET inhibit the descending of Bax,prolong its descending period andthen promote its expression,in order to weaken the inhibition of the apoptosis during 4 to 10 days after seizure and then promote the apoptosis during 10 to 14 days after.

埋线治疗通过抑制Bax蛋白表达的下降、延长其降至低峰值的时程，致使在癫痫发作后的中期(4d～10d)抑制大鼠海马神经元凋亡的作用减弱；继而通过促进Bax蛋白表达的回升，致使在癫痫发作后的远期(10d～14d)出现促进凋亡的作用。

Results:The results showed that the apoptosis of neutrophiles was promoted by the treatment of 1 μg/ml LPS and 1 μm A23187,in 6 hours and 12 hours respectively.The apoptosis of neutrophil was markedly delayed by a PLA2 inhibitor,4-bromophanacyl bromid (p-BPB 0.01～1 mM).

结果：脂多糖（LPS 1μg/ml）和钙离子载体（A23187 1 μmol/L）等PLA2激动剂分别在共同培养6 h和12 h之内促进中性粒细胞凋亡，而PLA2阻断剂4-溴苯甲酰溴甲烷(p-BPB 0.01～1 mmol/L)可明显减弱上述激动剂的促凋亡作用。

MATERAIL AND METHODS: The cell proliferation inhibition was measured by (3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromid,MTT) colorimetric assay. Morphological changes of EJ cells were observed by fluorescence staining of Hoechst 33258. Cell cycle and apoptosis were analyzed by flow cytometry.

材料与方法：四甲基偶氮唑蓝(3-[4，5-dimethylthiazol-2-yl]-2，5 diphenyl tetrazolium bromid，MTT)法检测EJ细胞增殖活力；荧光染色观察凋亡细胞的形态学变化；流式细胞术分析细胞周期及细胞凋亡；免疫细胞化学染色观察caspase-3 蛋白的表达。

The labia have now been sutured together almost completely.The drains and the Foley catheter come out at the top.

此刻阴唇已经几乎完全的缝在一起了，排除多余淤血体液的管子和Foley导管从顶端冒出来。

To get the business done, I suggest we split the difference in price.

为了做成这笔生意，我建议我们在价格上大家各让一半。