亡

Supervital NBS staining Nile Blue Sulphate , a lysosomotropic vital dye which stains embryonic apoptotic, but not necrotic cell death, is employed to identify the occurence and location of apoptosis.

活体胚胎的NBS染色 NBS是一种亲溶酶体染料，可使凋亡细胞着色，而坏死细胞不着色，因而可初步判定细胞凋亡发生的区域和程度。

To test the hypothesis, neontal rat cadiomyocytes Treat with heat shock(HS,42℃,2h) to induce the expression of HSP70,then teated with 0.5mmol/L H2O2. We first found H2O2 can reduced cadiomyocytes apoptosis and HSP70 only over-expression in the HS group, other two group only express HSP70 a little in the test of expression of HSP70.Then we tested the concentration of Ca2+, the [Ca2+ ] of CON was 192.224+6.654, the [Ca2+ ] of H2O2 group was 290.6918+8.922and the [Ca2+]of HS group was 214.2633+4.484.To further determine how HSP70 repaired the Ca2+ homestasis, we measured calcium transient of each group.

实验中应用MTT和流式细胞技术检测细胞的凋亡，发现损伤组的存活率明显低于其他2组，热休克组(Heat shock group，HS group)细胞存活率略低于正常组细胞；免疫组化结果表明只有在HS组才检测到阳性反应，即是HSP70的表达，其他2组均为弱阳性结果；应用离子成像技术对细胞内钙离子浓度进行测定，发现损伤组290.6918+8.922明显高于正常组192.224+6.654和HS组214.2633+4.484，HS组细胞内的钙离子浓度略高于正常组；为了进一步探讨HSP70对于ROS引起的心肌凋亡过程中钙离子的调控机制，又对各组的心肌细胞进行了钙瞬变的测定。

MethodsFlow cytometry was used to test the effects of Pinellia ternate proteins. The dosageeffect relationship was also studied.

方法应用流式细胞技术，检测半夏蛋白对人肝癌细胞Bel7402的诱导凋亡作用，并观察了其诱导凋亡作用的量效关系。

MethodsFlow cytometry was used to test the effects of Pinellia ternate proteins. The dosage effect relationship was also studied.

方法应用流式细胞技术，检测半夏蛋白对人肝癌细胞Bel 7402的诱导凋亡作用，并观察了其诱导凋亡作用的量效关系。

Suicide genes can convert the drug into toxical metabolic product, cytokines can enhance the antineoplastic and immunologic responses, angiogenesis inhibitors can inhibit the development of new vessels, and apoptosis related factors can induce the apoptosis of tumor cells.

自杀基因能将药物转化为毒性代谢产物，细胞因子能增强抗肿瘤免疫效应，血管生成抑制因子能抑制血管生成，凋亡相关分子能诱导细胞凋亡，这些基因在前列腺癌的基础和临床研究中均表现出一定的肿瘤生长抑制作用。

The animal models of subchronic cad-mium toxication and antagonism of selenium were established.

结果显示，亚慢性镉中毒可引起鸡肝组织细胞凋亡，加硒后可有效减少肝细胞凋亡数量及损伤程度。

Methods Human prostate cancer PC-3 cell, breast cancer ER-75-30 cell and lung cancer A549 cell were cultured in RPMI medium 1640.Light microscope,transmissional electron microscope, flow cytometer and immunohistochemical method were used to observe apoptosis morphological changes of three kinds of tumor cell lines, time and dose effect relationship and to detect expression of bcl-2 and bax.

方法人前列腺癌 PC-3细胞株、乳腺癌 ER-75-30细胞株和非小细胞肺癌 A549细胞株经培养后，采用光镜、电镜、流式细胞仪和免疫组化方法研究 188Re诱导 3种肿瘤细胞凋亡的形态学变化、时间剂量效应关系、细胞周期依赖性以及凋亡相关基因 bcl-2和 bax在其中的表达情况。

These data demonstrated that tributyrin can efficiently inhibit gastric cancer cell growth by inhibiting DNA synthesis and inducing apoptosis. The tributyrin-induced apoptosis is associated with down-regulation of Bcl-2 expression and up-regulation of Bax expression.

这些结果首次显示tributyrin能有效地通过抑制DNA合成和诱导细胞凋亡达到抑制胃癌细胞生长的作用，细胞凋亡与Bcl-2表达量下调和Bax表达量上升有关。

Cell activity were examined by trypan blue staining,morphological changes were examined by fluorescence microscope,and cell cycle were measured by flow cytometry.

结果表明，硝酸镧能够降低MDCC-MSB1的细胞活性，能够引起典型的凋亡形态学变化及细胞周期的改变，并呈现细胞凋亡的细胞型，变化均呈现时间-剂量效应。

By the Spearman＇s correlation analysis, apoptosis of renal tubuli was positively related to Bax protein expression and negatively to Bcl-2 protein expression.

经Spearman's相关性分析，肾小管细胞Bax蛋白表达与肾小管细胞凋亡呈正相关，而Bcl-2蛋白表达则与细胞凋亡呈负相关。

The labia have now been sutured together almost completely.The drains and the Foley catheter come out at the top.

此刻阴唇已经几乎完全的缝在一起了，排除多余淤血体液的管子和Foley导管从顶端冒出来。

To get the business done, I suggest we split the difference in price.

为了做成这笔生意，我建议我们在价格上大家各让一半。