亡

Hewever the caspase-3 expression was still very high in cortex. Conclusion: In KA model the hippocampus amygdala plays a key role in epilepsy. Therefore it provides a mimic optimal conditions for human temporal lobe epilepsy study. Its an effective method of studying the development, change and pathopoiesis of epilepsy. There is a close relationship between NPY and epilepsy. The regulation of NPY via VPA may be one of the antiepilepsy mechanism of mechanism of VPA. Moreover NGF can enhance the caspase-3 expression. Excito-amino acid can aggravate the apoptosis, which may be one of the reason for intractability of epilepsy.

KA模型因其海马杏仁核在癫痈中起核心作用，故具有模拟人类颗叶癫痈的理想条件，是研究癫痈发展变化、致痈机理的有效工具，NPY与癫病有着密切的联系，VPA调节NPY有可能是其抗癫痈机理之一，而NGF对caspase一3表达有促进作用，兴奋性氨基酸可造成细胞凋亡的增加，而凋亡细胞的增多有可能是癫痈久治不愈的原因之一。

Methods DCs were prepared from peripherad blood mononuclear induce d with gra-nulocyt e/macrophage colony-stimulating factor and interleukin-4. Apoptosis of glioma cells were induced with γ-radiation. We design these experiment groups including (1) coculture of DCs and apoptotic glioma cells and T cells,(2) coculture of DCs and U937 cells and T cells,(3) coculture of DCs and cultured glioma cell and T cells,(4) coculture of Des and T cell.

用粒－巨噬细胞集落刺激因子加白介素－4(IL-4)从人外周血分化、诱导DCs、γ-射线在体外诱导培养的人脑胶质瘤细胞凋亡，将DCs、T淋巴细胞和凋亡胶质瘤细胞共培养，同时设计不同类型肿瘤细胞（U937及培养胶质瘤细胞）作对照，分离、富集DCs、T淋巴细胞进行免疫应答及肿瘤细胞杀伤试验。

Methods DCs were pre pared from peripherad blood mononuclear induce d with gra-nulocyt e／macrophage colony-stimulating factor and interleukin-4.Apoptosis of he patochlangioma cells were induced with γ-radiation.We design these experiment groups including(1)coculture of DCs and apoptotic cancer cells and T cells,(2)co culture of DCs and necrosis cancer cells and T cells,(3)coculture of DCs and cul tured cancer cell and T cells,(4)cocultu re of DCs and T cell,(5)cocuture of DCs and cultured cancer cell.

用粒-巨噬细胞集落刺激因子加白介素-4(IL-4)从人外周血分化、诱导DCs、γ-射线在体外诱导培养的人胆管癌细胞凋亡，将DCs、T淋巴细胞和凋亡胆管癌细胞共培养，同时设计不同类型肿瘤细胞(坏死胆管癌细胞及培养胆管癌细胞)作对照，7d后，分离、富集DCs、T 淋巴细胞进行免疫应答及肿瘤细胞杀伤试验。

In this study, we investigate the effect of benzyl isothiocyanate and phenethyl isothiocyanate on human prostate cancer DU-145 cells, the results demonstrate that benzyl isothiocyanate and phenethyl isothiocyanate induced cause G2 / M phase arrest and apoptosis. These effects are in a dose- and time-dependent manners.

但是苯甲基异硫氰酸酯和苯乙基异硫氰酸酯造成人类前列腺癌细胞株DU-145的凋亡机转则尚未有较详细的报导，所以本研究即是探讨苯甲基异硫氰酸酯和苯乙基异硫氰酸酯造成人类前列腺癌细胞株DU-145的相关凋亡机转。

AIM: To investigate the antineoplastic effect of pholiota nameko polysaccharides and the mechanism of inducing K562 cell apoptosis. METHODS: MTT assay was used to determine the inhibitory rate of PNP with different concentrations on the proliferation of K562 cells, and the cell growth curve was drawn.

采用MTT法检测PNP对K562细胞增殖的抑制作用；绘制生长曲线；Hochest 33258染色计算凋亡率；RTPCR法、Western Blot方法检测凋亡相关基因Caspase3的表达。

And the Akt of activation carries gene of phosphation Bad, GSK-3, transcribe familial, IB, Caspases make close to wither dies gene misses work, have inhibitory nerve thereby yuan wither dies reach stimulative nerve yuan surviving action, reduce AD nerve then yuan many and missing, the pathology that improves AD changes.

而活化的Akt通过磷酸化Bad、GSK-3、转录因子家族、IB、caspases等使促凋亡基因失活，从而起到抑制神经元凋亡及促进神经元存活的功能，进而减少AD神经元的大量丢失，改善AD的病理变化。

Results 5-FU inhibits growth of Lovo cells; it is found that within a limited dosage and period of time, there has been an obvious increase in apoptosis cells under the photomechanical scope; the analysis of Lovo cells by FCM showed that after the induction of 5-FU, the percentage of apoptosis cells has increased; and FCM also displays dosage and time effects. Electrophoresis was obviously in a stripe shape of trapeziform.

结果 5－FU能抑制 Lovo细胞生长，在一定剂量和时间范围内，通过光镜观察，见凋亡细胞明显增多，经5－FU诱导后，用FCM 分析 Lovo细胞，发现凋亡百分率增加并显示剂量和时间效应。

Methods Expression of the survivin mRNA was evaluated by reverse transcriptase polymerase chain reaction in 76 NSCLC tumor samples,20 benign phymatoid lesion and 21 adjacent normal lung tissue samples Immunohistochemical assay was to detect the expression of P53,c myc,k ras proteins.

研究细胞凋亡的调节机制对于弄清肿瘤包括肺癌的发病机制及新的防治方法具有重要意义。survivin是最近发现的一种凋亡抑制蛋白，在已检测的几种肿瘤组织中表达上调[1 3] ，在肺癌中的表达目前报道甚少，其与

Both Fas and NO can lead chondrocyte apoptosis respectively and cause articular cartilage destruction. IGF-Ⅰ, TGF-β, bFGF, BMP and other growth factors are polypeptide agents that can influence cell activity by signal convection. They can accelerate chondrocyte proliferation and proteoglycan synthesis, play the local regulation action on formation and plerosis of bone and cartilage tissue by autocrine or paracrine. They have the ability to induce cartilage formation. Some investigations showed that growth factors can influence chondrocyte metabolism, synthesis of specific Ⅱ type collagen and proteoglycan by co-operation and inhibition. 1. 3 Situation of OA therapeutics The therapeutic methods of OA mainly comprised non-drug treatment, drug treatment, operation treatment, tissue and genetic engineering, et al. Drug treatment is the chief method among them.

若其活性发生改变，则将导致关节软骨基质成分的丢失和进行性破坏；软骨细胞凋亡与OA的发病密切相关，Fas与NO可各自独立介导软骨细胞凋亡，造成关节软骨破坏；IGF-Ⅰ、TGF-β、bFGF、BMP等生长因子是一组通过细胞间信号传递影响细胞活动的多肽因子，具有促进细胞生长、增殖与合成等作用，可通过自分泌或旁分泌方式对骨和软骨的形成和修复起局部调节作用，可促进软骨细胞增殖、分化与蛋白多糖的合成，具有较强的诱导软骨形成的能力，研究表明多种生长因子相互促进、相互抑制，以协同或拮抗方式影响软骨细胞代谢，影响软骨细胞特异性Ⅱ型胶原和蛋白多糖的合成分泌。

BMK can suppress HL-60 the growth and the induction cell perishes, the caspase-3 activation poisonously participated in the East Asian pliers scorpion to induce the process which the HL-60 cell weakly perished.

BMK能抑制HL-60的生长和诱导细胞亡，caspase-3活化参与了东亚钳蝎毒诱导HL-60细胞凋亡的过程。

We have no common name for a mime of Sophron or Xenarchus and a Socratic Conversation; and we should still be without one even if the imitation in the two instances were in trimeters or elegiacs or some other kind of verse--though it is the way with people to tack on 'poet' to the name of a metre, and talk of elegiac-poets and epic-poets, thinking that they call them poets not by reason of the imitative nature of their work, but indiscriminately by reason of the metre they write in.

索夫农 、森那库斯和苏格拉底式的对话采用的模仿没有一个公共的名称；三音步诗、挽歌体或其他类型的诗的模仿也没有——人们把&诗人&这一名词和格律名称结合到一起，称之为挽歌体诗人或者史诗诗人，他们被称为诗人，似乎只是因为遵守格律写作，而非他们作品的模仿本质。

The relationship between communicative competence and grammar teaching should be that of the ends and the means.

交际能力和语法的关系应该是目标与途径的关系。