亡

Objective To observe the expression of cytochrome Cand apoptosis-related gene Bax in hippocampus of posttraumatic stress disorderrats in order to investigate their effects and relationships.

目的 观察细胞色素C和凋亡相关基因 bax 在创伤后应激障碍大鼠海马神经元中的表达，探讨其在细胞凋亡中的作用及相互关系。

Methods:The proliferation inhibition of cantharides injections on LoVo cells were detected by tetrazolium.Annexin V-FITC were applied to study cell apoptosis of LoVo cells.FCM were applied to detect the expressions of the Fas and Bcl-2 protein.Results:Cantharides iniections could inhibit the proliferation of LoVo cells and could couse cell apoptosis.

四甲基偶氮唑盐测定药物对LoVo细胞抑制作用与时间和剂量的关系，Annexin V-FITC方法对细胞凋亡进行检测，流式细胞术检测细胞Fas、Bcl-2蛋白表达水平结果：MTT实验表明复方斑蝥注射液可抑制LoVo细胞的生长并诱导发生凋亡。

Objective To investigate the effects of Carthamus tinctorius on infarction volumes,apoptosis of neurons and bcl-2,caspase-3 expressions after acute injury of cerebral ischemia and reperfusion.

目的 探讨红花注射液对大鼠急性脑缺血再灌注损伤后梗死体积和神经元凋亡及凋亡相关蛋白bcl-2，caspase-3表达的影响。

Methods Human nasopharyngeal carcinoma ceil line CNE-1 with p53 mutation, was used in this study.

应用流式细胞仪和凋亡试剂盒测定不同条件下CNE-1细胞的凋亡比例。

However it was shown its eventually inhibiting effect on apoptotic myocyte cells during I〓 R〓h (P＜0.05); The PKC inhibitors chelerythrine and scutellarein have no effect on expression of Fas protein, whereas they can significantly upregulate the expression of Bcl-2 protein.

4PKC激活剂PMA可下调Fas基因的蛋白表达，对Bcl-2蛋白的表达也有抑制作用，它对缺血30min、再灌注24h后心肌凋亡细胞的最终影响为抑制效应(P＜0.05)，而PKC抑制剂CHE、灯盏花素对Fas基因蛋白表达无明显干预影响，但对Bcl-2蛋白表达呈显著上调作用，故对心肌细胞凋亡的抑制作用更为明显(P＜0.01)。

Objective To elucidate the patterns of chemotherapeutic drugs induced apoptosis and its role in cancer chemotherapy.

目的 了解化疗药物诱导细胞凋亡的规律，阐明细胞凋亡在肿瘤化疗中的作用和地位。

MethodsThe cells of human gastric cancer cell line SGC7901 was treated respectively with Chrysin at different concentration 10、20、40、80μM for 48h. The proliferation inhibitory rate was measured by MTT assay. Acridine orangestaining and flow cytometry were used to detect apoptosis. Western blot assay was used to detect the expression of apoptosis related genes NF拨B,Bcl2,Bax.

方法分别用10、20、40、80μM的ChR处理人胃癌细胞株SGC7901 48h，采用MTT比色法检测ChR对SGC7901细胞的增殖抑制效应；采用丫啶橙染色、流式细胞术检测ChR诱导SGC7901细胞凋亡的发生；应用Westernblot法检测凋亡相关基因NF拨B、Bcl2、Bax的蛋白表达，并用计算机图像分析软件进行半定量分析。

Results The IC50 of SPG-Rg3 was (155.7±0.71) mg·L-1,when the concentration of SPG-Rg3 was arranged from 37.5 to 600.0 mg·L-1,the growth inhibitory rate of MCF-7 cells was higher following the increase of its concentration,the cell growth was greatly inhibited compared with control group(P.05); cell cycle was changed,the cell number of S period increased compared with control group(P.01),the cell number of G2/M period decreased compared with control group(P.01),in experiment group there was an apoptotic apex before G1 apex,and the number of apoptotic cells increased; most of cell nuleus in SPG-Rg3 (150 mg·L-1)experiment group appeared yellow or chrysoidine fluorescence,contracted,beaded and crescent; the result of immunocellularchemical staining of caspase-8 indicated that caspase-8 protein had no expression in control group,but stronger expression in SPG-Rg3 group.

结果：SPG-Rg3 IC50为（155.70±0.71） mg·L-1，当SPG-Rg3浓度在37.5~600.0mg·L-1时，MCF-7细胞的生长抑制率随SPG-Rg3浓度的增加而增大，与对照组比较，细胞增殖受到明显抑制（P.05）；MCF-7细胞的生长周期也发生变化，S期细胞数增加，明显高于对照组（P.01，G2/M期细胞数则明显少于对照组（P.01；并且在G1峰前出现明显的凋亡峰，凋亡细胞数增加。

By FCM analy sing, p53 gene can induce cells apoptosis and make it G1 arrest; Cisplatine can induce cell apoptosis and make it G2 arrest.

流式细胞计数证实p53能诱导QBC939细胞发生凋亡并导致其发生G1期阻滞，顺铂能诱导QBC939细胞发生凋亡并导致细胞发生明显的G2期阻滞。

Objective:To investigate the relationship between cell proliferation,apoptosis and clinicopathological characteristics in primary lung cancer.

目的：研究人肺癌组织中细胞增殖状态、凋亡水平与临床病理生理特征之间的关系，探讨肿瘤细胞增殖与凋亡的关系。

We have no common name for a mime of Sophron or Xenarchus and a Socratic Conversation; and we should still be without one even if the imitation in the two instances were in trimeters or elegiacs or some other kind of verse--though it is the way with people to tack on 'poet' to the name of a metre, and talk of elegiac-poets and epic-poets, thinking that they call them poets not by reason of the imitative nature of their work, but indiscriminately by reason of the metre they write in.

索夫农 、森那库斯和苏格拉底式的对话采用的模仿没有一个公共的名称；三音步诗、挽歌体或其他类型的诗的模仿也没有——人们把&诗人&这一名词和格律名称结合到一起，称之为挽歌体诗人或者史诗诗人，他们被称为诗人，似乎只是因为遵守格律写作，而非他们作品的模仿本质。

The relationship between communicative competence and grammar teaching should be that of the ends and the means.

交际能力和语法的关系应该是目标与途径的关系。