亚该亚

The company further strengthened its North American product and service network by opening slitting and distribution terminals in Ontario, California and Wilkes-Barre, Pennsylvania in 2001 and 2004 respectively.

该公司进一步加强其在北美的产品和服务网络的开放纵剪和分销终端在安大略，加利福尼亚和威尔克斯巴里，宾夕法尼亚州在2001年和2004年分别。

The microorganism can utilize succinimide as a sole carbon source and nitrogen source, which indicates the presence of a complete transformation pathway of succinimide, and a hydantoin-cleavi...

真养产碱杆菌 1 1 2R4 能在以琥珀酰亚胺为唯一碳、氮源的培养基上生长，表明该菌中存在琥珀酰亚胺完整的转化途径。

This was brought up by the Kota Kinabalu MP Dr. Hiew King Cheu not long ago regarding the many months of total stoppage of work on the Tanjong Aru traffic junction flyover which caused heavy traffic jams during peak hours and caused safety hazard and inconvenience to road users.

与其同时亚庇国会议员邱庆洲博士曾经在不久前询问政府，有关停工多个月的丹容亚路天桥问题，由于该工程处在繁忙的交通繁路口，造成了严重交通堵塞，引起道路使用者的不便及有安全隐患，该项工程已经停工多个月。

The Teatro Tapia was built in 1832 (financed by subscriptions and by taxes on bread and imported liquor) and remodeled in 1949 and 1987 is one of the oldest theaters in the Western Hemisphere, was named for Alejandro Tapia y Rivera, the famous Puerto Rican playwright.

该剧院皮亚始建于1832年(由征订和面包及进口酒税)、1949年和1987改装是世界上最古老影剧院在西半球，被命名为杭德罗塔皮亚肽Y河畔波多黎各著名剧作家。

The structure of ArsA ATPase with its ligand, arsenite, either on membranes or in solution, was revealed. The current trimeric ring-like structure of this protein doubts the previous dimeric model and gives deeper insight on this protein's physiological roles.

对亚砷酸根复合体ArsA蛋白质分子结构的揭示显示了该蛋白在亚砷酸根存在情况下，在膜上和水溶液中都是环形三聚体，对前人的二聚体模型假说提出了置疑，并为解释它的生理功能提供了重要的结构信息。

The jagged University Range in Alaskas snow-draped St. Elias Mountains blushes red in the Arctic twilight. Many peaks in Wrangell-St. Elias National Park remain unnamed—and unclimbed—more than 20 years after the park was established.

该锯齿状大学射程在阿拉斯加的 S雪域披上圣埃利亚斯山变得红不红，北极曙光，许多山峰在遥远的圣埃利亚斯国家公园仍然是无名和unclimbed - 20余年后，公园成立。

Chicken IL-15(ChIL-15), Which was discovered in 2001, plays a main role in activating NK cells and CD8+ memory T cells, and may therefore have potential as a vaccine adjuvant.According to published ChIL-15 gene sequence, a pair of primers were designed and synthesized to clone ChIL-15 cDNA from ConA-activated chicken splenocytes by RT-PCR. Recombinant plasmid pUC19ChIL-15 carrying the ChIL-15 gene was constructed. The gene encoding mature ChIL-15 (mChIL-15) was amplified from pUC19ChIL-15 by PCR and cloned into pMD 18-T vector. After digested with Kpn I /Pst I , the mChIL-15 gene was subcloned into prokaryotic expression vector pPRoEX橦Ta and transformed into E.coli DH5a .The transformant was induced by IPTG, and the recombinant ChIL-15(rChIL-15) was expressed as a fusion protein with a histidine hexamer tag at the N-terminal end of the protein. Following expression, the protein was purified by ProBond?Nickel-Chelating Resin. The uninduced and induced protein lysates and the purified protein were separated by SDS-PAGE and transferred onto nitrocellulose membrane to identify rChIL-15 by Western blot. The rChIL-15 was used to immune the guinea pig to obtain rChIL-15 polyclonal antibody.

参考已发表的ChIL-15基因序列，设计合成引物，应用RT-PCR技术从刀豆球蛋白A活化的白来航鸡脾淋巴细胞中克隆ChIL-15 cDNA，将其与SmalⅠ酶切处理的pUC19载体连接，构建重组质粒pUC19ChIL-15；用PCR技术从pUC19ChIL-15中扩增出去信号肽的成熟ChIL-15(mChIL-15)基因，与pMD 18-T载体相连构建重组质粒pMDChIL-15；然后用KpnⅠ／PstⅠ双酶切下mChIL-15基因片段，定向亚克隆到经同样酶切处理的带有6个组氨酸标签的表达载体pP_RoEX~HTa中，构建重组质粒pP_RoChIL-15，对其测序确认读框正确后，转入大肠杆菌DH5α中诱导表达并进行纯化，用重组ChIL-15(rChIL-15)免疫豚鼠，制备多克隆抗体；再用HindⅢ／PstⅠ从质粒pP_RoChIL-15上切下mChIL-15基因，定向亚克隆到经同样酶切处理的杆状病毒转移载体pMelBacB中，经酶切、PCR和序列测定鉴定后，与杆状病毒线形化DNABac-N-Blue~(TM DNA共转染Sf9昆虫细胞，经三轮蚀斑纯化，构建重组杆状病毒rBac-ChIL-15，用该重组病毒感染处于对数生长期的Sf9细胞，按不同的时间收取样品，离心后对其上清和沉淀进行SDS-PAGE和Western blot分析。

Additional Green Business leaders speaking include Libby Reder of eBay's Environmental Initiatives, Woody Tasch of Slow Money, Bryan Welch from Ogden Publicatons, Priya Haji of World of Good, Joshua Onysko founder of Pangea Organics, Margot Fraser former CEO of Birkenstock USA, Joey Shepp of Earthsite, Terri Spath of RSF Social Finance and Adam Lowry of Method Products.

额外的绿色企业领导人来说，包括利比是eBay的环保措施，伍迪缓慢的货币，从布赖恩韦尔奇塔施奥格登Publicatons，普里亚的好，约书亚Onysko有机物的盘古，马戈弗雷泽勃肯美国，乔伊Shepp该公司前任CEO创始人世界哈吉瑞德Earthsite，特丽的无国界社会融资和亚当方法产品劳瑞斯帕思。

Complete genome sequences of 20 viruses in Bunyaviridae loaded from NCBI were analyzed by two biological softwares of DNAMAN and DNASTAR. The results indicated great variability in both nucleic acid sequence and protein structure between plant viruses and animal viruses:(1) only plant viruses could encored NSm in M genome;(2) the length of nucleic acid sequence and protein sequence was different;(3) GC content of nucleic acid of animal viruses was higher than plant viruses of that;(4) protein topology analysis by online software SMART discovered that there was significant difference for the structure of glycoproteins G(subscript nG between animal-infecting and plant-infecting viruses;(5) usually, protein of plant-infecting virus had more complicated structure, more low compositional complexity and had N-Signal peptides except INSV.

本文从NCBI数据库下载具有完整基因组序列的布尼亚科病毒的5个属20种病毒的序列，用生物学软件DNAman，DNAstar进行比对分析，发现布尼亚科病毒属中的植物病毒在核酸序列及蛋白结构上与动物病毒有很大差异：(1)只有植物病毒的M基因组能编码NSm运动蛋白；(2)植物病毒和动物病毒在核酸序列和蛋白质序列长度均有差异，表明该科病毒是进化速度较快的病毒；(3)植物病毒核酸序列的GC含量低于动物病毒；(4)通过SMART网络软件进行蛋白质拓扑结构分析发现植物病毒和动物病毒在糖蛋白GG的结构上存在显著差异；(5)植物病毒糖蛋白结构较为复杂，有较多的紊乱区域，除INSV外，其他病毒都具有N端信号肽。

Indonesia – Pray for an end to the migration of Muslims to West Papua, that the area will not become Islamized and that West Papua will be freed from the oppression of Indonesian control.

印尼—求神终止穆斯林迁移至西巴布亚岛的行动，使该地区不会被伊斯兰化，并使西巴布亚岛免於印尼掌控的压迫。

They weren't aggressive, but I yelled and threw a rock in their direction to get them off the trail and away from me, just in case.

他们没有侵略性，但我大喊，并在他们的方向扔石头让他们过的线索，远离我，以防万一。

In slot 2 in your bag put wrapping paper, quantity does not matter in this case.

在你的书包里槽2把包装纸、数量无关紧要。