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The carboxyl groups of QDES were used to couple the free amino groups of EAH Sepharose 4B with the carbodiimide coupling method, and immobilized density was 5μmol/ml.

通过碳二亚胺反应将四肽配基QDES固定在商品介质EAH Sepharose 4B上制备出了配基固定化密度为5μmol/ml的亲和吸附介质,并利用亲和色谱柱考察了其吸附蛋白质特性。

Methods: Hapten of dimethoate was synthesized, then it was conjugated to bovine sera albumin and ovalbumin to prepare immunogen and coating antigen respectively by carbodiimide method.

采用生产乐果的中间体硫磷醋与l, 4-二氨基丁烷反应合成乐果半抗原,利用碳二亚胺法,将半抗原与牛血清白蛋白及卵清蛋白偶联,分别制备免疫原和包被原。

Ferrocenecarboxylic-ssdeoxyribonucleic acid probe was made by chemically bounding FCA to terminal 5'-amino of oligonucleotide in the presence of a water-soluble carbodiimide. The hybridization reaction was conducted by immersing the immobilized-ssDNA electrode in FCA-ssDNA probe solution.

在偶联活化剂碳二亚胺的存在下,通过羧基二茂铁上的羧基与DNA分子上的氨基共价键合,将FCA标记在氨基修饰的寡聚核苷酸片段上,制备成FCA-ssDNA探针。

Three immobilization modes of antigen to the polymers in the pH-sensitive phase separation immunoassay were investigated and compared. The results showed that the immobilization mode in the presence of N-ethyl-N'-(3-dimethylaminopropyl)carbodiimide hydrochloride rendered the most desirable results. The immobilization efficiencies and immunological reaction activities of immobilized antigen of this mode were improved over the other two modes.

本文研究并比较了pH敏感相分离免疫分析中抗原在高分子上的三种固定化模式,结果显示由碳二亚胺偶联的固定模式效果最好,固定化效率、固定抗原的免疫反应活性较之另外两种模式均得到了提高。

The conjugation of MMS and Ab (ready for Reagent 1, R1): Chose the 3μm diameter Carboxyl MMS and use the carbodiimide cross-link technique activate the MMS with EDC and NHS in different concentration, then linked mice anti-HBsAg-IgG and mice anti-HCcAg-IgG on them.

磁微粒的活化及其与抗体的连接:选择粒径3μm的羧基化磁微粒,采用碳二亚胺共价交联法,在活化剂EDC与NHS的不同浓度组合条件下活化磁微粒,选择最适的活化剂浓度组合。

In order to overcome the problem of multidrug resistance in human epidemic carcinomata anti-adriamycin cells (KB-A-1), the antisense and antigene oligonucleotides were used to investigate their effectiveness on inhibiting the mdr1 gene expression. The effectiveness of antisense or antigene oligonucleotide on inhibiting the multidrug resistance was detected by MTT colometric assay and ELISA.

中文题名反义与反基因寡核苷酸及其萘二酰亚胺偶联物对靶基因表达的抑制作用研究副题名外文题名 The inhibition of the targeted gene expression by the antisense or antigene oligonucleotides and their naphthylimide-conjugated derivatives 论文作者李军生导师张元兴魏东芝教授学科专业生物化工研究领域\研究方向生物化学与分子生物学学位级别博士学位授予单位华东理工大学学位授予日期2001 论文页码总数106页关键词基因表达基因治疗反义寡核苷酸反基因寡核苷酸核酸馆藏号BSLW /2001 /Q78 /264 针对人表皮癌抗阿霉素细胞株(KB-A-1)的多药抗药性问题,本文从反义核酸和反基因核酸角度,通过MTT法检测细胞生长情况,ELISA法检测基因表达产物P-gp表达水平的变化,对寡核苷酸抑制肿瘤细胞MDR1基因表达的机制进行了探讨。

The results of FTIR and QCM measurement showed that Schiff bases could chemisorb on the copper surface through coordination, indicating that it is feasible to prepare SAMs of Schiff bases on the copper surface.

QCM和FTIR结果表明,希夫碱分子通过其中的亚胺基与铜的配位作用在铜表面发生化学吸附,从而形成自组装膜。因此用自组装技术在铜表面上制备希夫碱自组装膜是可行的。

The emission amount of tail gas in polyisobutylene succinimide production is very large,and the concentration of oil in the recovered chlorhydric acid by absorption is too high to sell.

针对聚异丁烯丁二酰亚胺生产工艺中废气排放量大、尾气吸收得到的盐酸含油量高、难以销售等问题,在分析原因的基础上,对尾气吸收系统进行了技术改进。

Results Cefoperazone/Sulbactam, Imipenem, Piperacillin/Tazobactam, Cefetamet and Quinolones had strong effects to resist Chromobacterium violaceum,while resistant effects of those antibiotics were not significant, including Ampicillin, Cefazolin, Gentamicin, Tobramycin, Aztreonam, Ceftriaxono, and Ceftazidime.

结果 12种抗菌药物中舒普深、亚胺培南、特治星、马斯平及喹喏酮类抗菌药物对紫色色杆菌均有较强的抗菌作用,而氨苄西林、头孢唑啉、庆大霉素、妥布霉素、氨曲南、头孢曲松和头孢他啶等抗菌药物抗菌效果不佳。

The antimicrobial susceptibility test showed that various pathogens had high resistant rate to penicillins and cephalosporins, but sensitive to Imipenem with a sensitive rate of 80%. The sensitive rate of the gram-positive cocci to Vancomycin was more than 85%.

药敏结果显示各种病原菌对青霉素类及头孢类抗生素有较高的耐药率,而对亚胺培南-西司他丁有较高的敏感率,达80%以上,革兰阳性球菌对万古霉素敏感率达85%以上。

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