亚磷酸

We confirmed the interaction of DNA-PKcs with Plk1 in vitro and vive using GST-pull down and co- immunoprecipitation.

2首次确定了对于DNA-PKcs发挥功能至关重要的磷酸化形式(pT2609)在细胞周期不同时期的亚细胞定位。

After thecomplete genome extraction of the strain was performed, the genomic DNA was partiallydigested by restriction enzyme Sau3AⅠ, the DNA fragments from 1 to 5Kb was clonedinto prokaryote expression vector pET-28a-c, and transformed host bacteria. The resultsshowed that we succeeded in constructing the gene expression library of haemophilusparasuis serovar 5, which is fundamental for the study of advanced gene screening. Inaddition, primer design was performed based on haemophilus influenzae in this study. In addition, PCR was performed by using genomic DNA of haemophilus parasuisserovar 5 as the template. The results demonstrated that we obtained two neo-gene:23SrRNA gene(conserved gene belonging to the large-subunit of ribosome) and adenylatecyclase gene(encodes adenylate cyclase and participates in converting adenyl nucleosidetriphosphate to cyclic adenosine3",5"-monophosphate). Furthermore, the phylogeneticanalyses between the species was performed, and neighbor-joining tree was constructedbased on comparison of 23S rRNA gene sequences, so it was illuminated betweenHaemophilus parasuis and other species in molecular evolution relationship.

选择我国流行优势菌株副猪嗜血杆菌血清5型地方株为研究对象，提取细菌基因组DNA，用限制性内切酶Sau3AⅠ对基因组DNA进行部分酶切，回收大小为1～5Kb的DNA片段，将其连接入原核表达载体pET-28a-c，最后转化宿主菌，结果成功地构建了基因表达文库，为后续的基因筛选工作奠定基础；另外，本研究选择嗜血杆菌属的流感嗜血杆菌为参考对象进行引物的设计，以副猪嗜血杆菌血清5型地方菌株的基因组DNA为模板，进行PCR扩增反应，结果表明成功地获得两个新基因：23S rRNA基因(存在于核糖体大亚基中的保守性基因)和腺苷酸环化酶基因(负责将腺嘌呤核苷三磷酸转变为环腺苷酸)，并进一步做了不同物种之间的分子系统发育分析，构建了基于23S rRNA基因的邻接法系统发育树，阐明了副猪嗜血杆菌与其它菌种的分子进化关系。

Immobilization of the anticoagulative or antithrombogenic biomolecule has been considered as one of the important methods to improve the blood compatibility of artificial biomaterials. This paper describes research to improve the blood compatibility of silicone rubber and polyethylene films.

利用血小板粘附实验对接枝膜的血液相容性作了评价，实验结果表明壳聚糖膜表面接枝水溶性高分子N—双取代的亚甲基磷酸壳聚糖后，其血液相容性得到很大的提高。

The method is based on the catalytic effect of NO~-_2 on oxidation of bromophenol red by potassium bromate in H_3PO_4 mediun.

基于稀磷酸介质中亚硝酸根催化溴酸钾氧化溴酚红的褪色反应，建立测定痕量亚硝酸根的新方法。

Glucose-6-phosphatase system (G6PA) is the key enzyme that catalyses the reaction glucose production in cells, which is composed by 5-6 subunits.

葡萄糖-6-磷酸酶（G6PA）是催化细胞内产生葡萄糖的关键酶，此酶由5-6个亚基构成。

Its main products include:ZOLEDRONIC ACID, ADAPALENE, SIMDAX, VORICONAZOLE, J ACID UREA, J ACID UREA SODIUM SALT, 1,7-CLEVE'S ACID, 1,6-CLEVE'S ACID, 1,5- DINAPHTHYL ISOCYANATE, 5- INROINDOLE,----2-METHOXYIMINO-2-（FURYL-2-YL）ACETIC ACID AMMONIUM SALTand etc.

部分产品远销美国、德国、英国、印度、日本等十几个国家和地区，主要产品有：唑来磷酸、阿达帕林、左西孟旦、伏立康唑、猩红酸、猩红酸钠盐、双介酸、1，7克力夫酸1，6克力夫酸、1，5-萘二异氰酸酯、5-硝基吲哚、甲氧亚胺基呋喃乙酸铵等。

We found that under acid condition, selenite radical combined with active hydroxide radical and amino-group of chitooligosaccharides to form chitooligosaccharides - Se compound molecule, the remaining group complex interacted with the negative sodium tripolyphosphate via electrostatical interactation to form nanoparticles with the size about 100nm.

通过透射电镜、粒径分析仪等方法表征，发现壳寡糖-硒纳米颗粒平均粒径为100nm左右，在酸性条件下，亚硒酸根与壳寡糖单元上活性羟基和氨基化合，形成壳寡糖-硒复合分子，剩余的基团与带负电的多聚磷酸钠复凝聚，在4℃下能够稳定保存，25℃以上壳寡糖溶胀而致纳米颗粒发生分解。

In the forth part, in the presence of chiral phosphoric acid, highly enantio-enriched (up to 98% ee)α-aryl glycine derivatives could be synthesized via asymmetric transfer hydrogenation of the α-aryl imino esters using Hantzsch ester as the hydrogen source.

第四部分工作中，利用Hantzsch酯作为氢源，高对映选择性地实现了手性磷酸催化芳基酮酸酯亚胺的不对称仿生氢化反应，以最高98％的对映选择性得到苯甘氨酸衍生物。

The results showed polymeric diaryliodonium salts were effective aryl ion transfer reagents. Thirdly, a new type polymer supported hypervalent iodine reagents poly { [4-Hydroxy iodo] styrene} has been developed. It was an effective oxidative reagent, and could be applied to the one pot synthesis of 4-arylthiazoles.

然后，我们以聚苯乙烯羟基对甲苯磺酰氧基碘苯为原料，成功制备了一类新型聚合物高碘试剂—聚苯乙烯负载的炔基碘盐，并研究了其作为炔基转移试剂的有效性：(1)该试剂可对硫、氮、硒等负离子进行亲电炔基化反应，合成炔基亚砜、0，0-二烃基二硫代磷酸炔基酯、1-苯乙炔基苯并噻唑以及炔基硒醚。

The starch-cyclodextrin microspheres were prepared by using starch/β-cyclodextrin as raw material, normal hexane as dispersed phase, Span60 and Tween60 as emulsifying, pentanol as assistant of emulsifying, and metaphosphoric acid trisodium salt as linking agent.

纳米微球以可溶性淀粉和β-环糊精为原料，选用正己烷为油相，Span60和Tween60为乳化剂，正戊醇为助乳化剂，三偏磷酸钠为交联剂，以对亚甲基蓝的去除率为指标进行了单因素和正交试验。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。