亚磷酸

The invention discloses a preparing method of bacillus alcaligenes strain Alcaligenes sp., CCTCC No.M206121 of degraded o-nitrophenol, which comprises the following steps: allocating inorganic salt culture medium with dibasic sodium phosphate, monobasic potassium phosphate, manganese sulfate, copperas, addex-magnesium, calcii chloridum, blue copperas, white copperas and hydrogen dioxide solution; adjusting pH value; sterilizing under high temperature; enriching bacterial; sampling from sludge; allocating suspending liquid with soil sample and inorganic salt; seeding into ortho-nitrophenol fresh organic salt culture medium with finite volume seeding quantity; keeping the temperature; culturing on shaker; getting enriched culture; separating and purifying enriched culture in LB solid culture medium.

本发明公开了一种降解邻硝基酚的产碱杆菌菌株及制备方法，产碱杆菌为Alcaligenes sp。，CCTCC No.M206121。其制备步骤是：首先是配制无机盐培养基，成分为磷酸氢二钠、磷酸二氢钾、硫酸锰、硫酸亚铁、硫酸镁、氯化钙、硫酸铜、硫酸锌和双蒸水，调pH值高压灭菌；其次是菌种的富集，从污泥中取样，将土样与无机盐培养基配制成悬浮液，将悬浮液以一定体积接种量接种到含邻硝基酚的新鲜无机盐培养基中，恒温，摇床培养，获富集培养物；第三是将富集培养物在LB固体培养基中分离和纯化。

Up-regulated proteins under salt stress include an ABC transporter permease, glycerol-3-phosphate permease, pyrimidine nucleotide transporter, formate dehydrogenase, and down-regulated proteins include succinate dehydrogenase iron-sulfur subunit, flavoprotein subunit, cytochrome b-556 subunit, and a hypothetical membrane protein similar to charperone DnaJ. Some of these changes were further verified by enzyme activity assay.

盐胁迫诱导上调表达的蛋白包括ABC型转运蛋白，3-磷酸甘油透性酶，嘧啶核苷转运蛋白和甲酸脱氢酶；下调表达的蛋白包括琥珀酸脱氢酶铁硫亚基、黄素蛋白亚基、细胞色素b556亚基，以及分子伴侣DnaJ的同源蛋白，酶活力测定结果表明胁迫条件下上述蛋白的活性变化与表达量变化相一致。

The protein encoded by this sequence was deduced to be consisted with 213 amino acids, signal peptide sequence from 1 to 17 position, and transmembrane regions from 171 to 190 position, whose grand average of hydropathicity is computed to be 0.031, secondary structure is composed by alpha helix (57.28%), extended strand (6.57%) and random coil (36.15%), subcellular localization was cytoplasmic by CELLO v.2.5. There are some function sites predicted by PROSCAN software, such as one N-glycosylation site (151-154aa), one protein kinase C phosphorylation site (193-195aa), two casein kinase II phosphorylation sites (155-158aa and 173-176aa), and one N-myristoylation site (97-102aa).

推测编码蛋白由213个氨基酸组成，信号肽序列位于1-17aa，亲水性指数为0.031，跨膜区域位于171-190aa，二级结构由-螺旋（57.28%）、延伸主链（6.57%）和无规卷曲（36.15%）组成；亚细胞定位于细胞质，含有N-糖基化位点1个(151-154aa)，蛋白激酶C磷酸化位点1个(193-195aa)，酪蛋白激酶Ⅱ磷酸化位点2个(155-158aa and 173-176aa)，N端酰基化位点1个(97-102aa)。

Experiment by using thiophosphorylation and fluorescent labeling proved subunit A of vacuolar H〓-ATPase was one of the phosphorylated proteins located in tonoplast.

以硫代磷酸化为模型并引入荧光标记，证明V型H〓-ATPase的A亚基是液泡膜上可以被磷酸化的蛋白质之一。

For pre-selection of iron compounds that ferrous sulfate, ferrous chloride, ferrous fumarate, carbonyl iron, amino acid chelated iron, iron asporotate, fenic chloride and ferric pyrophosphate were added in reconstituted skim milk to undergo in vitro digestion, respectively, and then the selected iron compounds were added in reconstituted whole milk to compare the iron bioavailability by estimating dialyzable ferrous iron, dialyzable total iron, nondialyzable and total ferrous iron, respectively.

取硫酸亚铁、反丁烯二酸亚铁、氯化亚铁、艰基铁、Iron asporotate、Amino acid chelated iron、氯化铁与焦磷酸铁等不同铁剂添加於脱脂还原乳中进行体外消化试验，并筛选生物利用性佳之铁剂分别添加於全脂还原乳中，以可透析二价铁、可透析总铁、未透析二价铁及总二价铁等作为评估之指标。

For pre-selection of iron compounds that ferrous sulfate, ferrous chloride, ferrous fumarate, carbonyl iron, amino acid chelated iron, iron asporotate, fenic chloride and ferric pyrophosphate were added in reconstituted skim milk to undergo in vitro digestion, respectively, and then the selected iron compounds were added in reconstituted whole milk to compare the iron bioavailability by estimating dialyzable ferrous iron, dialyzable total iron, nondialyzable and total ferrous iron, respectively.

取硫酸亚铁、反丁烯二酸亚铁、氯化亚铁、艰基铁、Iron asporotate、Amino acid chelated iron、氯化铁与焦磷酸铁等不同铁剂添加於脱脂还原乳中进行体外消化试验，並筛选生物利用性佳之铁剂分别添加於全脂还原乳中，以可透析二价铁、可透析总铁、未透析二价铁及总二价铁等作为评估之指標。

Methods Before and 30 days after treatment with histotherapy,T lymphocyte subpopulation,complement and complement activation segment were measured in 17 patients by ELISA and APAAP techniques.

方法采用ELISA法和碱性磷酸酶抗碱性磷酸酶法，观察17例银屑病病人采用组织疗法治疗前及治疗30d后外周血T淋巴细胞亚群补体及活化片段的变化，同时观察了组织疗法的效果。

Nineteen spots were changed significantly after FA treatment.Thirteen proteins were identified by peptide mass fingerprinting or peptide sequence analysis,including putative nucleoside diphosphate kinase,elongation factor 1-gamma,triosephosphate isomerase,60S acidic ribosomal protein P0,heat shock protein 75 kDa,similar to heat shock 70kD protein binding protein,annexin I,hypothetical protein FLJ34423,microtubule-actin crosslinking factor 1,lamin B2,ATP synthase alpha chain,mitochondrial precursor,proteasome subunit alpha type 6.These identified proteins involved in energy metabolism,translation and RNA processing,protein folding,redox regulation,cell structure and cell signaling.

双向凝胶电泳结果显示，甲醛刺激后19个蛋白斑点发生变化，肽指纹图谱及肽序列标签鉴定了其中13个蛋白斑点，已鉴定的蛋白包括二磷酸核苷酸激酶、延长因子1-γ，磷酸丙糖异构酶、60S酸性核糖体蛋白P0、75kDa热休克蛋白、70kD热休克蛋白样结合蛋白、钙依靠磷脂结合蛋白I、假想蛋白FLJ34423、微管-肌动蛋白交叉连接因子1、核纤层蛋白B2、ATP合成酶α链、蛋白酶体α亚基6，这些蛋白功能涉及转录调节、蛋白折叠、信号传导、能量代谢、细胞骨架等各个方面。

Thirteen proteins were identified by peptide mass fingerprinting or peptide sequence analysis,including putative nucleoside diphosphate kinase,elongation factor 1-gamma,triosephosphate isomerase,60S acidic ribosomal protein P0,heat shock protein 75 kDa,similar to heat shock 70kD protein binding protein,annexin I,hypothetical protein FLJ34423,microtubule-actin crosslinking factor 1,lamin B2,ATP synthase alpha chain,mitochondrial precursor,proteasome subunit alpha type 6.These identified proteins involved in energy metabolism,translation and RNA processing,protein folding,redox regulation,cell structure and cell signaling.

双向凝胶电泳结果显示，甲醛刺激后19个蛋白斑点发生变化，肽指纹图谱及肽序列标签鉴定了其中13个蛋白斑点，已鉴定的蛋白包括二磷酸核苷酸激酶、延长因子1-γ，磷酸丙糖异构酶、60S酸性核糖体蛋白P0、75kDa热休克蛋白、70kD热休克蛋白样结合蛋白、钙依赖磷脂结合蛋白I、假想蛋白FLJ34423、微管-肌动蛋白交叉连接因子1、核纤层蛋白B2、ATP合成酶α链、蛋白酶体α亚基6，这些蛋白功能涉及转录调节、蛋白折叠、信号传导、能量代谢、细胞骨架等各个方面。

Bone marrow mesenchymal stem cells could differentiate into osteoblasts, adipocytes and neural like cells with osteoblast inductor (β-sodium glycerophosphate, dexamethasone, vitamin C), lipoblast inductor (dexamethasone, 3-isobutyl-1-methylxanthine, bovine insulin, indometacin) and serum-free medium inductor (dimethyl sulphoxide, butylated hydroxyanisole) respectively. Osteoblast marker (alkaline phosphatase, osteocalcin mRNA, calcium node), adipocyte marker (lipid droplet, PPAR γ-2mRNA) and neural cell-like marker (nissl body, neuron specific enolase, neurofilament protein) were respectively determined by the immunohistochemical method, polymerase chain reaction and immunocytochemical method.

分别采用成骨细胞诱导剂（β-甘油磷酸钠，地塞米松，维生素C）、成脂肪细胞诱导液（地塞米松，甲基异丁酸黄嘌呤，牛胰岛素，吲哚美辛）及二甲基亚砜和羟基丁酸苯甲醚无血清培养基诱导剂干预细胞向成骨、脂肪、神经细胞分化，经免疫组织化学染色、PCR、免疫细胞染色方法检测成骨标志物（碱性磷酸酶、骨钙素mRNA、钙结节）、脂肪标志物（脂滴、PPARγ-2mRNA）、以及类神经标志物（尼克氏体、神经烯醇化酶、神经丝蛋白）。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。