亚目的

The Omp25 gene of wapiti's Brucella abortus was amplified by RT-PCR and sequenced, Then the interesting gene was subcloned into pET28a for expression. The results showed that the full length of Omp25 gene was 642bp, which encoded 213 amino acids.

运用RT-PCR技术从马鹿布氏杆菌分离株中扩增出Omp25基因，将其克隆入pMD18-T中进行核苷酸序列测定和遗传变异分析，并将目的基因亚克隆到大肠杆菌表达载体pET28a中诱导表达。

Objective: To study changes of subtype of T lymphocytes and ACTH in different trait-anxiety levels of new recruits under acute stress.

目的：研究急性应激对不同特质焦虑新兵的T淋巴细胞亚群及ACTH的影响。

AIM:To study the effect of ONOO- on the airway epithelial injury.

目的：探讨过氧亚硝基阴离子对气道上皮细胞的损伤作用。

Aristotle, too, defines Nature as purposive activity, purpose is what is immediate and at rest, the unmoved which is also self-moving, and as such is Subject.

贺译（ p13）：亚里士多德曾规定自然为有目的的行动，同样我们认为，目的是直接的、静止的、不动的东西；不动的东西自身却能引起运动，所以它是主体。

Count Ilya Andreitch bethought himself of this diplomatic stratagem to give the future sisters-in-law greater freedom to express their feelings to one another (so he told his daughter afterwards), but also to avoid the possibility of meeting the prince, of whom he was afraid.

伊利亚·安德烈伊奇想出了这套外交手腕，其目的无非是给未来的小姑和嫂嫂留有谈话的余地（后来他把这桩事告诉她女儿），其目的无非是避免碰见他所惧怕的公爵。

Methods:The total RNA was extracted from mouse RAW264.7 cells stimulated by LPS.The sequence including the whole length of HMGB1 was amplified by RT-PCR and inserted into pMD-19T.The combinant vector was used as a template for PCR which was cloned into vector pMD-19T,then subcloned into expression vector pET-26b with pelB signal sequence and His-Taq sequence.After transforming E.coli BL21(DE3) and four hours induction by IPTG,HMGB1 expression confirmed by SDS-PAGE and the purification was performed by Ni2+-chelate affinity chromatograph.

脂多糖刺激后的RAW264.7细胞，提取总RNA，经RT-PCR扩增出含HMGB1的目的片段，克隆于pMD-19T载体，再亚克隆至含有pelB引导肽及His-标签肽的高效表达载体pET-26b，转化大肠杆菌BL21（DE3），经IPTG诱导后行SDS-PAGE鉴定目标蛋白表达，用镍鳌合琼脂糖凝胶亲和层析法分离纯化含His-标签肽的目的蛋白。

Methods:The total RNA was extracted from mouse RAW264.7 cells stimulated by LPS.The sequence including the whole length of HMGB1 was amplified by RT-PCR and inserted into pMD-19T.The combinant vector was used as a template for PCR which was cloned into vector pMD-19T,then subcloned into expression vector pET-26b with pelB signal sequence and His-Taq sequence.

脂多糖刺激后的RAW264.7细胞，提取总RNA，经RT-PCR扩增出含HMGB1的目的片段，克隆于pMD-19T载体，再亚克隆至含有pelB引导肽及His-标签肽的高效表达载体pET-26b，转化大肠杆菌BL21（DE3），经IPTG诱导后行SDS-PAGE鉴定目标蛋白表达，用镍鳌合琼脂糖凝胶亲和层析法分离纯化含His-标签肽的目的蛋白。

The combinant vector was used as a template for PCR which was cloned into vector pMD-19T,then subcloned into expression vector pET-26b with pelB signal sequence and His-Taq sequence.After transforming E.coli BL21(DE3) and four hours induction by IPTG,HMGB1 expression confirmed by SDS-PAGE and the purification was performed by Ni2 -chelate affinity chromatograph.

脂多糖刺激后的RAW264.7细胞，提取总RNA，经RT-PCR扩增出含HMGB1的目的片段，克隆于pMD-19T载体，再亚克隆至含有pelB引导肽及His-标签肽的高效表达载体pET-26b，转化大肠杆菌BL21（DE3），经IPTG诱导后行SDS-PAGE鉴定目标蛋白表达，用镍鳌合琼脂糖凝胶亲和层析法分离纯化含His-标签肽的目的蛋白。

First, to take ideology as a weapon was the starting point of the revolutionary drama-"the Left Drama"while to pay attention to human and the conflict between human and the society, which led to the probation of the connotation of the conflict consciously was the core of sub-current realism. Second, the main current realism had a strong revolutionary"proletariat"aim and political sense-objective sense while the sub-current realism focused on people and setting up characters. Third the main current realism laid too much emphasis on the sense of mission of its time which led to the tendency of formulizing and generalizing while the sub-current realism paid attention to the firm objectivity and to imitate life as their aesthetic ideal, and as a result the sub-current realism produced vigorous works. CHAPTER 4 Realistic Chinese modern drama experienced a hard and winding way of development. It was even worse after the People Republic of China was established.

本章还从三个基本方面入手，论述了主流的现实主义与非主流的现实主义内质的差异与不同的发展趋向，即作为一种武器的思想是主流的革命现实主义中&左翼戏剧&的出发点，与注重人与命运、人与现实的冲突，从而自觉追求冲突内涵的挖掘是非主流现实主义的核心；主流的革命现实主义中&普罗列塔利亚式&强烈的革命目的&目的意识论&与非主流现实主义的倾心于人物、注重人物塑造的审美原则；主流的革命现实主义所强调的时代使命感而产生的公式化、概念化倾向与非主流的现实主义所注重的客观性、以摹仿生活真实为审美理想而产生的具有生命力的话剧剧作。

Objective: To observe the postponed effect of mild hypothermia on neuron apoptosis process after traumatic brain injury, and study the positive significance at cellular level.

目的：观察亚低温对脑创伤后神经元程序性细胞死亡进程的延缓作用，从细胞水平测试亚低温治疗的意义。

We have no common name for a mime of Sophron or Xenarchus and a Socratic Conversation; and we should still be without one even if the imitation in the two instances were in trimeters or elegiacs or some other kind of verse--though it is the way with people to tack on 'poet' to the name of a metre, and talk of elegiac-poets and epic-poets, thinking that they call them poets not by reason of the imitative nature of their work, but indiscriminately by reason of the metre they write in.

索夫农 、森那库斯和苏格拉底式的对话采用的模仿没有一个公共的名称；三音步诗、挽歌体或其他类型的诗的模仿也没有——人们把&诗人&这一名词和格律名称结合到一起，称之为挽歌体诗人或者史诗诗人，他们被称为诗人，似乎只是因为遵守格律写作，而非他们作品的模仿本质。

The relationship between communicative competence and grammar teaching should be that of the ends and the means.

交际能力和语法的关系应该是目标与途径的关系。