亚目的

OBJECTIVE: To observe the effects of T lymphocyte cells and subsets of rat Peyer's patch on the intestinal mucosa by earlier oral feed with medicined diet in severe scalded rats.

目的：观察早期药膳饮食对烫伤大鼠肠道肠黏膜Peyer's结T淋巴细胞及其亚群的影响。

Objective To explore the prevalence of sub-health among medicos and the influencing factors,so as to put forward countermeasures.

目的 探讨医科大学学生亚健康现状及发生原因和影响因素，为制订对策提供科学的理论依据。

Furthermore, Binary stars have been neglected in most studies of the integrated light of the stellar populations with the EPS methods. However in real clusters and galaxies over 50% stars are in binaries, and binary interaction can result in some important classes of objects, such as blue straggles and subdwarf B stars (sdB, also referred as extreme horizontal branch ) which contribute significantly to the integrated light.

另外，多数演化星族合成模型在对星族进行研究时没有考虑双星作用，而在真实星团或星系中50％以上的恒星属于双星系统，双星相互作用可以产生一些重要天体，例如蓝离散星和B型亚矮星，而这类天体对积分光谱有重要影响，因此本论文研究目的之二就是在演化星族合成模型中考虑双星作用。

AIM To study the T cell receptor and Vβ genes subfamilies expression characters in recognizing and killing hepatoma antigen and its relationship to activity of protein tyrosine kinase.

杨红目的探讨在T细胞活化信号传递中特异性识别和杀伤肝癌抗原的T细胞受体Vβ基因亚家族的表达特征，及与蛋白质酪氨酸激酶活性的关系。

Objective The purpose of this study was to evaluate the clinical results of 402 porcelain veneers bonded with the Panavia F system.

目的：通过对402颗烤瓷贴面的临床观察，评价帕娜碧亚F粘结系统对烤瓷贴面的粘结效果。

Objective: To observe the effects of Losartan in serum uric acid and urinary albumin excretion rate of the patients with diabetic nephropathy.

目的 ：观察氯沙坦( losartan，科素亚)对 2型糖尿病肾病患者血尿酸( serum uric acid，UA)、尿白蛋白排泄率( urine albumin excretion rate，UAER)的影响。

Objective To investigate the changes of T lymphocyte subsets, B cells and natural killer cells in peripheral blood of patients with secondary syphilis and its clinial significance.

目的 了解T淋巴细胞亚群、B细胞及自然杀伤细胞在二期梅毒患者中的改变，探讨这些免疫细胞在梅毒发病机理中的作用。

Objective To investigate the significance of changes in T lymphocyte subsets, B cells and natural killer cells of peripheral blood in patients with systemic lupus erythematosus.

目的 了解T淋巴细胞亚群、B细胞及自然杀伤细胞在活动期SLE患者中的改变，探讨这些免疫细胞在SLE发病机理中的作用。

Objective To investigate the significance of changes in T lymphocyte subsets, B cells and natural killer cells of peripheral blood in patients with systemic lupus erythematosus.

广东医学000515 目的了解T淋巴细胞亚群、B细胞及自然杀伤细胞在活动期SLE患者中的改变，探讨这些免疫细胞在SLE发病机理中的作用。

The PCR products were examined by agarose gel electrophoresis. The target gene fragments were purified by gel extraction kit and ligated to cloning vector pMD18-T. The recombinant vectors were transformed into host strain E. coli K802 by lithium chloride method, screened and identified with PCR and restrictive enzymatic digestion. Their sequences were confirmed by DNA sequencing.(2) sTWEAK1 gene was subcloned into expression vector pProEx HTb and transformed into E. coli BL21. sTWEAK2 gene was subcloned into expression vector pMAL-C2x and transformed into E. coli TB1. The recombinant vectors were screened and identified with PCR and restrictive enzymatic digestion. The recombinant fusion proteins were induced to express with IPTG, detected by coomassie brilliant blue-stained SDS-polyacrylamide gel electrophoresis , and confirmed by Western blot analysis.(3) The sTWEAK1 fusion protein was purified with Ni-NTA Spin Kit.(4) The biological activity was assayed on transformed and tumor cells by microplate photometer after crystal violet or sulfur rodamine B staining.(5) The contents of IL-8 in the supernatant of 1990 cell cultures were determined by ELISA.(6) The morphological changes of the sensitive cells were observed by light and transmission electron microscopies.(7) The cell cycle and apoptotic rate were assayed by flow cytometry in 1990 and M85 cells.(8) The effect of fusion proteins on induction of NF-κB in 1990 and LOVO cells was detected with Dual-Luciferase Reporter Assay system.(9) The TWEAK gene was subcloned into Adeno-X Viral DNA with pShuttle vector and transfected into HEK293 cells by lipofectamine method.

(1)本研究用RT-PCR方法，从人组织细胞总RNA中扩增可溶性TWEAK胞外区（sTWEAK1和sTWEAK2）的cDNA序列及全长编码序列，用琼脂糖凝胶电泳分析PCR产物，胶回收目的基因片段，连接到pMD18-T克隆载体中，转化大肠杆菌K802，PCR和酶切筛选阳性克隆，全自动DNA测序验证序列；(2)sTWEAK1和sTWEAK2分别亚克隆到pProEx HTb和pMAL-C2x表达载体中，分别转化大肠杆菌BL21和TB1，PCR筛选和酶切鉴定，阳性克隆用IPTG诱导表达，表达产物用SDS-PAGE分析和Western blot验证融合蛋白；(3)用NTA-Ni Spin试剂盒初步分离纯化sTWEAK1融合蛋白；(4)用体外培养的肿瘤细胞和正常对表达产物进行活性检测，贴壁细胞用结晶紫染色法，悬浮细胞用磺酰罗丹明B染色法，酶标仪检测OD值；(5)敏感细胞用ELISA法检测细胞培养上清中IL-8的含量；(6)用光镜和电镜观察敏感细胞死亡和细胞凋亡情况；(7)用流式细胞仪分析表达产物对敏感细胞凋亡率和细胞周期的影响；(8)用双荧光素酶报告基因检测法，测定表达产物对敏感细胞NF-κB的影响；(9)用pShuttle穿梭质粒将TWEAK重组到腺病毒载体上，用脂质体转染法转染HEK293细胞，PCR鉴定重组质粒。

We have no common name for a mime of Sophron or Xenarchus and a Socratic Conversation; and we should still be without one even if the imitation in the two instances were in trimeters or elegiacs or some other kind of verse--though it is the way with people to tack on 'poet' to the name of a metre, and talk of elegiac-poets and epic-poets, thinking that they call them poets not by reason of the imitative nature of their work, but indiscriminately by reason of the metre they write in.

索夫农 、森那库斯和苏格拉底式的对话采用的模仿没有一个公共的名称；三音步诗、挽歌体或其他类型的诗的模仿也没有——人们把&诗人&这一名词和格律名称结合到一起，称之为挽歌体诗人或者史诗诗人，他们被称为诗人，似乎只是因为遵守格律写作，而非他们作品的模仿本质。

The relationship between communicative competence and grammar teaching should be that of the ends and the means.

交际能力和语法的关系应该是目标与途径的关系。