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The invention discloses a preparing method of bacillus alcaligenes strain Alcaligenes sp., CCTCC No.M206121 of degraded o-nitrophenol, which comprises the following steps: allocating inorganic salt culture medium with dibasic sodium phosphate, monobasic potassium phosphate, manganese sulfate, copperas, addex-magnesium, calcii chloridum, blue copperas, white copperas and hydrogen dioxide solution; adjusting pH value; sterilizing under high temperature; enriching bacterial; sampling from sludge; allocating suspending liquid with soil sample and inorganic salt; seeding into ortho-nitrophenol fresh organic salt culture medium with finite volume seeding quantity; keeping the temperature; culturing on shaker; getting enriched culture; separating and purifying enriched culture in LB solid culture medium.

本发明公开了一种降解邻硝基酚的产碱杆菌菌株及制备方法,产碱杆菌为Alcaligenes sp。,CCTCC No.M206121。其制备步骤是:首先是配制无机盐培养基,成分为磷酸氢二钠、磷酸二氢钾、硫酸锰、硫酸亚铁、硫酸镁、氯化钙、硫酸铜、硫酸锌和双蒸水,调pH值高压灭菌;其次是菌种的富集,从污泥中取样,将土样与无机盐培养基配制成悬浮液,将悬浮液以一定体积接种量接种到含邻硝基酚的新鲜无机盐培养基中,恒温,摇床培养,获富集培养物;第三是将富集培养物在LB固体培养基中分离和纯化。

Then,in the bovine serum albumin buffer solution containing disodium hydrogen phosphate and citric acid,ferrite/polyacrylamide was added with crosslinker glutaraldehyde and then be enwrapped by BSA.

首先在反相微乳液体系中制备出Fe3O4/PAM磁性微球;然后在牛白蛋白的磷酸氢二钠-柠檬酸缓冲溶液中,以戊二醛为交联剂,加入Fe3O4/PAM微球,使蛋白质特异性吸附在微球上。

Disodium hydrogen phosphate dodecahydrates possesses the largest mass heat storage capacity among the low temperature phase change materials, which are classified as inorganic, organic and then: composites, yet it experiences phase segregation and super cooling when melting and hinders its application in latent heat storage system.

低温相变材料可分为无机、有机物及其复合物。十二水磷酸氢二钠(Na_2HPO_4·12H_2O)是其中质量储热密度最大的一种,但由于熔化时发生相分离,而妨碍了它的实际应用。

Like thionamic acid, alkyl benzene sulfonic acid, nonyl phenol oxygen ethylene ether, ethylene diamine four ethanoic acid two sodium, oxalic acid, deionized water and so on.

如氨基磺酸、烷基苯磺酸、壬基酚氧乙烯醚、乙二胺四乙酸二钠、草酸、去离子水等。

Meanwhile, the standard experiment on addition of small molecular organic arsenics revealed that the small molecular organic arsenics such as cacodylic acid, disodium methylarsonate were detected to be inorganic arsenic by the two detection methods with high recovery efficiency.

同时,试验中用小分子有机砷进行加标试验,结果表明:二甲基胂酸、甲基胂酸二钠等小分子有机砷对两种测定方法的影响很大,会把小分子有机砷作为无机砷检测出来,且检出回收率很高。

The product includes the licorice frost, licorice paste, licorice acid, Acetyl Glycyrrhetic Acid, Crude Disodium Glycyrrhizininate, Dipotassium Glycyrrhizininate, Monopotassium Glycyrrhizininate, Diammonium GlycyrrhizininateDiammonium Glycyrrhizininate, Monoammonium Glycyrrhizininate etc.

产品有甘草霜、甘草膏、甘草酸、乙酰甘草次酸、甘草酸单铵盐、甘草酸单钾盐、甘草酸二钾盐、甘草酸三钾盐、甘草酸二钠盐、高纯甘草酸等品种。

Preservatives in general are troublesome to crystalline structures and especially disodium EDTA.

一般的防腐剂对水晶结构是有麻烦的,特别是乙二胺四乙基二钠

Bi2Te3 nanocapsules have been synthesized by the aqueous chemical route at 65℃ with the addition of disodium ethylenediaminetetraacetate salt.

采用65℃低温湿化学合成方法,在添加乙二胺四乙酸二钠的情况下,制备了Bi2Te3纳米囊。

A proper composition of the plating solution was obtained as follows: copper sulfate 16 g/L,EDTA·2Na 30 g/L,glyoxylic acid 13 g/L,2,2'- bipyridine 10 mg/L.

优化工艺条件:硫酸铜16 g/L,乙二胺四乙酸二钠30 g/L,乙醛酸13 g/L,2,2′-联吡啶10 mg/L,pH 12.5,温度 40℃。

In addition, MSG, or baking soda with the bogey and alkali use, so as not to become one of the glutamic acid disodium sodium glutamate lose flavor.

此外,味精,或与忌和碱使用小苏打,以免成为谷氨酸二钠,谷氨酸钠一失去味道。

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