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Methods By the bioinformatics analyzing tools in bioinformatics webs site,a Ta CRISP 2 fulllength gene from the Taenia saginata asiatica fulllength cDNA plasmid libratory was identified and the coding region sequence and the characteristics of the deduced protein were analyzed.The coding region of Ta CRISP was amplified with PCR,endonuclease digestion and cloned into the prokaryotic expression vector pET30a and then expressed in E.coli BL21 with IPTG induction.

用生物信息学方法从亚洲牛带绦虫成虫全长cDNA质粒文库中识别TaCRISP的全长编码基因并预测编码蛋白质的各种结构与功能;将其编码区序列克隆到原核表达载体pET-30a上,测序鉴定重组质粒,之后进行诱导表达,表达产物经十二烷基磺酸钠-聚丙烯酰胺凝胶电泳鉴定。

Bone marrow mesenchymal stem cells could differentiate into osteoblasts, adipocytes and neural like cells with osteoblast inductor (β-sodium glycerophosphate, dexamethasone, vitamin C), lipoblast inductor (dexamethasone, 3-isobutyl-1-methylxanthine, bovine insulin, indometacin) and serum-free medium inductor (dimethyl sulphoxide, butylated hydroxyanisole) respectively. Osteoblast marker (alkaline phosphatase, osteocalcin mRNA, calcium node), adipocyte marker (lipid droplet, PPAR γ-2mRNA) and neural cell-like marker (nissl body, neuron specific enolase, neurofilament protein) were respectively determined by the immunohistochemical method, polymerase chain reaction and immunocytochemical method.

分别采用成骨细胞诱导剂(β-甘油磷酸钠,地塞米松,维生素C)、成脂肪细胞诱导液(地塞米松,甲基异丁酸黄嘌呤,牛胰岛素,吲哚美辛)及二甲基亚砜和羟基丁酸苯甲醚无血清培养基诱导剂干预细胞向成骨、脂肪、神经细胞分化,经免疫组织化学染色、PCR、免疫细胞染色方法检测成骨标志物(碱性磷酸酶、骨钙素mRNA、钙结节)、脂肪标志物(脂滴、PPARγ-2mRNA)、以及类神经标志物(尼克氏体、神经烯醇化酶、神经丝蛋白)。

The group treated with 3 mmol/L malonate sodium was selected as damage group.

选择3mmol/L丙二酸钠作为损伤组进行下一步实验。

If 0.01 M sodium malonate is added to actively respiring mitochondria that are using pyruvate as fuel source, respiration soon stops and a metabolic intermediate accumulates.

如果0.01M丙二酸钠加入到以丙酮酸为能源并正在进行活跃呼吸作用的线粒体中,呼吸作用很快停止,代谢中间产物累积下来。

Then the contents of brain tissue water, malonyl diadehyde and the activities of Na+-K+-adenosine triphosphatase (Na+-K+-ATPase), Ca2+-Mg2+-adenosine triphosphatase (Ca2+-Mg2+-ATPase), CuZn-superoxide dismutase and mitochondrial Mn-superoxide dismutase were measured.

测定脑组织含水量、丙二醛含量、钠钾三磷酸腺苷酶(Na~+-K~+-ATPase)、钙镁三磷酸腺苷酶Ca~(++-Mg~-ATPase、铜锌超氧化物歧化酶以及线粒体锰超氧化物歧化酶的活力。

Hign-purity manganese carbonate from the manganiferous waste water was produced from the manufacture of 1,4-dihydroxy benzene, especially the preparations from waste water containing plenty of natrium and sulphate were studied.

以对苯二酚生产中副产物含锰废水为原料,研究了生产高纯碳酸锰时除杂质钠盐与硫酸根的工艺,并研究了影响杂质含量的诸因素,如温度、锰离子浓度、加料方式等。

The precursor of sodium titanate nanotubes is prepared by: reacting titanium dioxide, metatitanic acid or titanate ester in 20-80 wt.% NaOH aqueous solution at 100-140 deg.C for 12-72 h, washing with water, filtering and drying.

用于反应的前驱体纳米管钛酸钠由二氧化钛、偏钛酸或钛酸酯与20-80wt%NaOH水溶液在100-140℃反应12-72h后用水洗去NaOH,过滤、干燥制成。

Acetylpyruvic acid methyl ester can be synthesized by the condensation of dimethyl oxalate and acetone in the presence of sodium methoxide.

叙述了在催化剂甲醇钠的存在下,以草酸二甲酯和丙酮为原料合成乙酰丙酮酸甲酯的工艺。

An individual with methylmalonic acidemia who is critically ill must first be stabilized; the base deficit of the individual with metabolic acidosis is calculated and corrected using intravenous bicarbonate solutions containing glucose, and measurements of electrolytes, venous or arterial blood gases, urine output, and serum sodium concentrations are monitored.

治疗:患有甲基丙二酸血症的个体,若病情严重应首先稳定病情;代谢性酸中毒的患者的碱缺失需要计算,并用静脉注射含有葡萄糖的碳酸氢盐溶液进行矫正,电解质测量、静、动脉血气、排尿量和血清钠浓度需要监控。

Pingxiang Iron and Steel Co., Ltd. has developed a de-cyanide pretreatment technology with molysite, but the concentration of CN - at a range of 10~18 mg/L in the wastewater after treating is still too high as for germ in dephenolization. The influence o...

根据对活性污泥的驯化过程对脱酚活性污泥影响的研究,得出适于细菌生存繁殖的运行参数为磷酸二氢钠加入量 12kg/d以上,葡萄糖 9kg/d以上,自沉污泥指数约 2 0mL/10 0mL ,回流污泥指数约 30mL/10 0mL ,温度为 2 5~35℃。

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