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The results show that all the hmgr genes from pigs, cattle and chickens are astable enzyme proteins with the same gene sequence characteristics and encoding protein and physical and chemical properties; all the amino acid sequences have a high degree of homology, containing two conservative HMG-CoA binding motifs and two NADPH binding motifs; all the amino acid sequences, containing signal peptide, are secreted proteins; they are all hydrophobic transmembrane proteins with five transmembrane domains; random coil and α-helix are the main structural elements in the HMGR polypeptide chain; all the predictions of three-dimensional structure are V- shaped; there are more closer evolution relationship between the HMGR gene from pigs and cattle.

结果表明:猪、牛和鸡HMGR基因序列特点及编码蛋白质理化性质基本相同,均属不稳定类酶蛋白;保守结构域氨基酸序列具有高度的同源性,均含有保守的2个β-羟基-β-甲基戊二酸单酰辅酶A结合基序和2个还原性烟酰胺腺嘌呤二核苷酸结合基序;均有信号肽,属分泌型蛋白质;属于跨膜的疏水性蛋白,均有5个跨膜结构域;无规则卷曲和α-螺旋是HMGR多肽链中的主要结构元件;三维结构预测都呈"V"字形;猪和牛HMGR基因的进化关系最近。

The preliminary stiochiometric analysis showed that when the enzyme metabolized EDDA, two acetyl groups were removed and ethylenediamine and two glyoxylates were produced in the presence of oxygen.

然后对该酶促反应进行了定量分析:结果显示在IDA氧化酶的作用下每代谢一分子的EDDA将生成二分子的乙醛酸及一分子的乙二胺,同时也检测到过氧化氢的生成。

The concentration of fluorine in the LPD-SiO/sub 2/ change with the concentration of hydro-fluorosilicic, and by the FTIR spectroscopy we find the concentration of about 1.5M will get the maximum concentration of fluoric incorporation.

在LPD含氟二氧化矽的实验中,调变六氟矽酸的浓度将可得到不同含氟量的二氧化矽膜,藉由FTIR的频谱可知,1.5M的浓度可得最高之含氟量。

A series of novel chiral aminophosphine ligands with binaphthyl backbone and partially reduced binaphthyl backbone were synthesized from optically pure NOBIN and H〓-NOBIN respectively. The effects of binaphthyl backbone and substitutes at the phosphorous atom on Pd (0)-catalyzed allylation of racemic 1, 3-dipheylprop-2-en-1-yl acetate with dimethyl malonate were systematically investigated.

从NOBIN出发合成了一系列膦上含有不同取代基的氮膦配体MAPs以及含有被部分还原的联萘骨架的氮膦配体H〓-MAPs,将此两类配体运用于钯催化的乙酸1,3-二苯基烯丙基酯与丙二酸二甲酯的反应中,考查了膦上取代基和配体骨架对反应的影响。

Then, aspartic acid was used for the enrichment of tryptic peptide mixture of C57BL/6J mouse liver lysate and good results were also obtained which demonstrated that aspartic acid was a promising non-specific binding inhibitor for complex biological samples.

将添加天冬氨酸的二氧化钛富集体系应用于鼠肝全蛋白质磷酸肽的富集中,同样取得了很好的效果,表明天冬氨酸在复杂的生物样本的磷酸肽富集中也同样具有良好的应用前景。

Typically,0.2 mmol of cd solution, and 0.4 mmol of mpa solution were mixed in a 40 ml solution and the ph of the solution was 二氰基丙酸乙酯 to 10.0 by solution was palced in a three-necked flask and was deaerated.

通常情况下, 0.2浓度的镉环硫乙烷,和0.4浓度的mpa的混合溶液中40巯基丙酸甲酯溶液和ph值的溶液是调整,以10.0的溶液放置在是在三制作乙酸钙中,并除去溶液中的气体。

Then we applied these chrial ligands to asymmetric borane reduction of w-bromoacetophenone and diketones.

将其应用于前手性单酮及二酮的还原中,其中双方酰化脯氨酸在1,6-二苯基-1,6-己二酮的还原反应中可获得最高为90%的ee值。

The influences of the reaction conditions on the grafting rate of dibutyl maleate to black N330 and the grafted black N330 on the physical properties of NR vulcanizate were investigated.

探讨炭黑N3 3 0表面固相接枝顺丁烯二酸二丁酯的反应条件对DBM接枝率的影响及炭黑接枝DBM对NR硫化胶物理性能的影响。

In order to meet the urgent needs of the environmental protection and avoid pollution from the source, the synthesis of WPU containing low VOC is very important.

为了满足环境保护需求,从源头上解决涂料污染的问题,选用结构组成不同的二元醇和1,6-己二酸,制备了一组支化度不同的低聚物聚酯二醇。

The proportion of the phase transfer catalyst and tertiary amine are usually in the range of about 1-5 and about 0.01-5.0 mole percent, respectively, based on total dihydroxyaromatic compound and dicarboxylic acid employed. In the second phosgenation step, the pH is raised to at least 10 and a stoichiometric phosgene excess of at least 5% is introduced.

相转移催化剂和叔胺的比例以二羟基芳族化合物和二羧酸的总量为基础计通常分别在约1―5和0.01―5.0摩尔百分数的范围内,在第二步光气化步骤中,pH值升高到至少约10,并导入超过化学计量约至少5wt%的光气。

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