二色性

A method for determinating the activities of pectinase was proposed in this paper. It can produce the red-brown amino compounds when 3,5-dinitrosalicylic acid and aldehyde were heated together. The amino compounds had maximum absorbency at 540nm. In certain range, there was a good linear relationship between the amount of reducing sugar and the color of the reaction mixture.

针对QB1502-92标准测定方法测定高活力果胶酶、固定化果胶酶活力存在的实验误差大，重现性及灵敏度差的缺陷，提出了利用3，5-二硝基水杨酸与醛糖共热产生棕红色的氨基化合物，在一定范围内还原糖的量和含有呈色氨基化合物的反应液颜色深浅成正比的原理，在540nm下测其吸光度，从而计算出果胶酶活力的测定方法，并对测定步骤、影响因素及测定中可能出现的问题进行了试验研究。

On a ZORBAX Stable Bound (2.150 mm, 1.8 m), the rutin、quercitrin、quercitrin-3-O-giucoside and quercitrin-3-O-galactose could be separated completely with 0.05 mol/L potassium dihydrogen phosphate and methanol as mobile phase (40:60,V/V) at a flow rate of 0.3 ml/min and detected with a photodiode array detector .

在色谱柱为ZORBAX Stable Bound (2.150 mm， 1.8 m)，流动相为0.05 mol/L的磷酸二氢钾缓冲溶液-甲醇(40：60，V/V)，流速为0.3 ml/min，在检测波长为360 nm的色谱条件下，芦丁、槲皮素、槲皮素-3-O-葡萄糖苷和槲皮素-3-O-半乳糖苷分离效果好，方法灵敏度高，重现性好，用于测定雪莲果叶中的上述几种黄酮芦丁型黄酮，效果满意。

Omeprazole racemate was resolved respectively by simulated moving bed chromatography and batch chromatography with the coated cellulose triphenylcarbamate as chiral stationary phase and ethanol containing 0.1% diethylamine as mobile phase.

用纤维素三苯基氨基甲酸酯涂敷型手性固定相和含0.1%二乙胺的乙醇溶液，研究了模拟移动床色谱和间歇色谱对奥美拉唑对映体的拆分。

Six experimental stages were designed in our procedure, those are:(1) metabolite recovery and tested sample preparation: the metabolites were recovered by Amberlite XAD-2 absorption, followed by MeOH elution and solvent concentration;(2) antioxidant detection and strain selection: samples were quantitatively analyzed by the inhibition effects on formation of lipid peroxides and TBARS to screen the strains able to produce antioxidants. According to the established screening methods, we chose out a strain of actinomycetes, designed as AMBL-029C;(3) antioxidant purification: the fermentation broth was recovered by a series of separation techniques including centrifugation, Amberlite XAD-2 absorption, followed by MeOH elution and a successive TLC purification. The resulting primary purified compound [temperately designed as AMBL-029C-TS] was further analyzed by HPLC to monitor its purity;(4) physical-chemical characteristics: judging from the acid-base fractionation experiments, and the pH and temperature stability tests, the compound was deduced to be a acidic compound with the properties of low polarity and highly pH and temperature stable;(5) mechanism of the antioxidant: in comparison with some other known antioxidants, TS was subjected to investigate its antioxidant mechanism, together with BHT,-tocopherol, as well as two streptomyces metabolites, homogentisic acid and -phenylpyruvic acid, which were previously isolated as the natural antioxidants in our laboratory.

针对本实验目的，我们设计了以下的实验步骤﹔（1）二次代谢物回收及检测样本处理：我们将发酵所得的培养上清液，利用疏水吸附性树酯Amberlite XAD-2吸附回收，并以甲醇溶离及真空减压浓缩脱水等方式处理，以取得提供抗氧化活性筛选之检测样本；（2）抗氧化活性检测及菌种筛选：以「过氧化脂质」和「硫丙二醯尿」的生成量进行定性定量分析以作为抗氧化物质生产菌筛选之用；经此筛选程序，我们选获了具有抗氧化物质高生产力的菌株，命名为AMBL-029C；（3）抗氧化物质的分离纯化：针对生产菌株的发酵回收处理液，以矽胶薄层色层分离法经物质层析纯化后，并以高效能液相层析法(High performance liquid chromatography；HPLC)分析物质可得一初级纯化物质，命名为AMBL-029C-TS；（4）抗氧化物质的物理化学性质分析：由酸碱转溶（acid-base fractionation）实验得知，此抗氧化物质属於中低极性的强酸性物质，对温度（37℃-100℃）及酸碱度（pH3.0-13.0）均表现出高稳定性；（5）在抗氧化机制探讨方面，我们针对数种不同的抗氧化机制进行探讨，即： 1。

The method uses first phase law to determine 63 DVT patient contrasts normally with 30 the F Ⅻ C of the group, use law of thing of the heart that deliver quality to detect at the same time zymogen of serous fine dissolve is active , law of enzymatic couplet immunity measures sex of antigen of content of activation of zymogen of methodize fine dissolve (depressor of content of activation of zymogen of dissolve of T-PAAg), fine - 1 antigen sex (PAI-1Ag) and immunity compare chaotic law to determine serous D-2 gets together body content.

方法采用一期法测定63例DVT患者和30例正常对照组的FⅫC，同时采用发色底物法检测血浆纤溶酶原活性，酶联免疫法测定组织型纤溶酶原激活物抗原性、纤溶酶原激活物抑制剂-1抗原性(PAI-1Ag)及免疫比浊法测定血浆D-二聚体含量。

The results of research showed that compound ferrous flocculant has simple preparation and not any second pollution matter is produced to the environment, it has big and stout floes formation and fast speed sedimentation while dealing with waste water, it is positively charged according to the electrophoresis, it belongs to cationic flocculant, can gives better coagulation results in turbidity removal, color removal, the maximum removal rate of turbidity and color are 95.4% and more than 81.7%, respectively. There are interactions between polysilicic acid and hydrolyzed ferrous species, the compound ferrous with appropriate Si/Fe not only but also can lower the water treatment cost. The compound ferrous removes colloid particles in water by charge oxidation-reduction, neutralization and furl mechanism.

研究结果表明：复合亚铁制备工艺简便，无二次污染；处理水样时产生的矾花大，密实、沉降速度快；复合亚铁带电性为正，为阳离子型絮凝剂；复合亚铁具有较好的除浊、脱色能力，除浊率及脱色率最高分别可达95.4％及81.7％；在复合亚铁中，聚硅酸与亚铁的水解产物间存在着相互作用，合适的硅铁摩尔比有利于提高混凝效果，而且还可以降低投药成本；复合亚铁是通过氧化还原、吸附电中和及卷扫三种作用机理起混凝作用的。

It has also been discovered that, before complete purification, the synthetic HWTX-Ⅰ, after oxidative treatment, showed wider and more asymmetric peaks than the native HWTX-Ⅰ that was denatured and then renatured under the same experimental conditions, which suggests that there is even greater conformational unhomogeneity in the synthetic HWTX-Ⅰ.

推测除了在合成HWTX-Ⅰ复性过程中有少数分子发生二硫键错配外，还有部分分子在固相合成中发生了消旋作用，需要交替采取多种色谱法才能完全分离纯化复性后的合成多肽产物。

Comprehensive two-dimensional gas chromatography is a new method, which is especially suitable to separate volatilizable and semi-volatilizable samples.

全二维气相色谱作为一种新的色谱技术，尤其适用于挥发、半挥发性复杂样品的分离。

Lycopene content of 33 watermelons materials involved in different flesh color and ploid were determinated by Spectrophotometry in this experiment,the results showed that: lycopene content of the white flesh of watermelon was lowest,the highest content was dark red flesh,among white flesh,orange flesh,golden yellow flesh,pink flesh,red and dark red flesh of watermelon material,There was a positive correlation between lycopene content and flesh color.

采用分光光度法对33个西瓜材料进行茄红素含量的测定，材料涉及不同的瓤色和倍性。结果表明：在白瓤、橙黄瓤、金黄瓤、粉红瓤、红瓤和深红瓤的西瓜材料之间，白瓤西瓜茄红素含量最低，深红瓤西瓜含量最高，茄红素的含量与瓤色大致成正相关。其中深红瓤二倍体西瓜材料中S292、VS8-44的茄红素含量比较高，分别为6.372 mg/100g鲜重和6.350 mg/100g鲜重，三倍体材料中最高的是VS6-19，茄红素含量为6.684 mg/100g鲜重。

This research utilized the method of organic synthesis, researching different amounts and positions of hydroxy-substituted benzoic acid and serotonin into amide reaction, so as to produce a series of polyhydroxy substituted N-benzoylserotonin derivatives (compound 1-10). Accordingly, this study conducts the following four tests. I. The activity of antioxidant and the experiment of ability to scavenge DPPH free radical, II. The ability of overall antioxidant—the activity of TEAC, III. The activity test of inhibit Tyrosinase, and IV. The study of absorbing UV / VIS Spectrophotometers. This study use trolox, arbutin, and octyl salicylate as the control groups, and to test the ability of anti-oxidation, the activity of inhibit Tyrosinase, and the ability of absorbing UV. This research ultimately intends to investigate the applied value of those compounds into cosmetics.

本研究主要利用有机合成的方法，将不同数量及不同位置的羟基取代苯甲酸化合物与色洛冬宁进行醯胺化反应后，得到一系列的多羟基取代之N-色洛冬宁苯甲醯胺衍生物（化合物1 ~ 10），进行四种不同活性测试方法：一、清除DPPH自由基能力抗氧化活性测试；二、总抗氧化能力活性测试；三、抑制酪胺酸酶活性美白测试；四、紫外线／可见光光谱仪吸收测试，并以水溶性维生素E、熊果素、水杨酸辛酯当作对照组，探讨其抗氧化能力、抑制酪胺酸酶的活性能力与吸收紫外线能力，再进一步探讨其应用於化妆品上的价值。

Plunder melds and run with this jewel!

掠夺melds和运行与此宝石！

My dream is to be a crazy growing tree and extend at the edge between the city and the forest.

此刻，也许正是在通往天国的路上，我体验着这白色的晕旋。