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To realize the molecular compositions and bio-spinning mechanisms of spidroin in spider major ampullate, the molecular weight and amino acid compositions of spidrion solution were measured and compared with that of dragline silk. The molecular conformations and morphologies of spidroin and dragline silk in different spinning phases were determined by circular dichroism spectroscopy and laser Raman spectroscopy.

本文以广泛分布于我国各地的大腹园蛛为对象,研究了大腹园蛛主腺体内丝蛋白溶液的分子量和氨基酸组成,并利用圆二色光谱和拉曼光谱分析了蜘蛛在不同成丝阶段丝蛋白的分子构象和聚集态结构的变化,以期了解蜘蛛主腺体内丝蛋白的分子组成及其生物纺丝机制。

In this research, the spider silk from the major ampullate gland of native Nephila pilipes was collected via the forced-silking apparatus at the reeling speed of 3 m/min. The obtained silk was dissolved in hexafluoro-isopropanol. The influence of pH and temperature over the secondary structure of the silk protein in solution state was examined via Circular Dichroism. The effect of silk concentration and electrical field strength on the structure conformation of silk protein during film casting process was investigated via FT-IR. During the drying process, the increase of silk concentration and the presence of electric field tend to form β-sheet and β-turn. Finally, the dried film of silk protein was treated with different temperatures and cation solutions to study the post-treatment effects.

本研究所采用之蜘蛛丝蛋白质系取自本土品种人面蜘蛛之大囊壶腺体,以3 m/min之卷丝速度强迫取丝(forced-silking)收集蜘蛛丝,再将纤维溶於六氟异丙醇溶剂中配制成溶液,以圆二色光谱仪分析在不同 pH 值与温度条件下丝蛋白质在溶液中之二级结构变化,再将蜘蛛丝溶液以不同浓度及电场强度下乾燥成薄膜后,以傅立叶红外线光谱仪检测分析丝蛋白质在固体状态下之二级结构,最后以不同温度加温、离子溶液浸泡等条件对乾燥所得之薄膜进行后处理,并分析其二级结构之变化。

L. bicolor has high value in directoring the large-scale plant in flat saline soil.

试验结果说明二色补血草具有较强的耐盐能力,适合在滩涂盐碱地推广种植。

The effects of different concentrations of various hormones on the root number and length of Limonium bicolor were studied and the survival ratio and growth of plantlets in different substrates were evaluated.

摘 要:以二色补血草为材料,研究了不同浓度激素配比对试管苗生根量及根长的影响,以及生根苗在不同移栽基质中的成活率和生长状况。

In both leaf and root of L. bicolor, the expression of LbMT2 was induced by CuSO4, CdCl2, NaCl, and cold, but inhibited by PEG stress.

结果表明, CuSO4、CdCl2、NaCl和低温处理均能诱导LbMT2基因在二色补血草的根和叶中的表达,而PEG处理则抑制了LbMT2在根和叶中的表达。

The expression of LbMT2 gene in L. bicolor in response to CuSO4, CdCl2, NaCl, cold, and PEG was further investigated using real time quantitative PCR.

利用实时定量PCR方法研究了二色补血草在CuSO4、CdCl2、NaCl、低温和PEG胁迫下不同时间该基因的表达模式。

Taking leaf and hypocotyl of Limonium bicolor as the explant, the system of rapid propagation were established, the influences of hormone concentration to induce the tissue culture and adventitious buds were studied.

方法]以二色补血草的子叶与胚轴作为外植体,建立快速繁殖体系,研究不同激素浓度对诱导愈伤组织、不定芽以及生根率的影响。

TPP gene plays an important role in synthesis of trehalose. The cloning of TPP gene lays a solid basis for investigating the biosynthesis of trehalose in L. bicolor.

海藻糖-6-磷酸磷酸酯酶在海藻糖合成过程中起关键作用,该基因的克隆为研究海藻糖在二色补血草中的合成奠定了基础。

In view of studying the suitable type of medium, probable way of explant regeneration and suitable condition of culture for tissue culture in Brunfelsia latifolia , an experimentation was established in which stem segments and a series of different medium were employed.

以茎段为材料,采用不同培养基,研究确定适合二色茉莉组织培养的培养基类型,植株再生的可能途径,以及适宜的培养条件。

It caused aggregation of purple membrane fragments at very low concentration. This effect might be attributed to its positive charges because cationic surfactant DTAB acted the same. On the other hand, melittin seemed to have behavior similar to CHAPS which has a rigid cholane ring,both had little effect on the conformation of retinal chromporphores.In consideration of the two-dimensional crystalline structure of purple membrane,melittin may form a rigid conformation such as α-helix on the surface of the membrane.

通过与三种在结构和电荷上不同的表面活性剂与紫膜的作用相比较,可以年出蜂毒作为带正电荷的分子与同样带正电的表面活性剂DTAB在引起紫膜凝聚方面表现相同;但在对紫膜可见光区的吸收光谱和圆二色谱的影响上却与具有刚性结构的CHAPS相似,表明蜂毒可在紫膜表面以一种刚性较大的构象存在,不能进入膜蛋白流水区的深层。

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Finally, according to market conditions and market products this article paper analyzes the trends in the development of camera technology, and designs a color night vision camera.

最后根据市场情况和市面上产品的情况分析了摄像机技术的发展趋势,并设计了一款彩色夜视摄像机。

Only person height weeds and the fierce looks stone idles were there.

只有半人深的荒草和龇牙咧嘴的神像。

This dramatic range, steeper than the Himalayas, is the upturned rim of the eastern edge of Tibet, a plateau that has risen to 5 km in response to the slow but un stoppable collision of India with Asia that began about 55 million years ago and which continues unabated today.

这一引人注目的地域范围,比喜马拉雅山更加陡峭,是处于西藏东部边缘的朝上翻的边框地带。响应启始于约5500万年前的、缓慢的但却不可阻挡的印度与亚洲地壳板块碰撞,高原已上升至五千米,这种碰撞持续至今,毫无衰退。