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Methods: into a series of 10 ml colorimetric tube were added different aliquots of the 1,8 -dihydroxyanthraquinone standard solution,2.0 ml of naac-hac buffer solution(ph=3.60),2.0 ml of ethylacetate.

于10.0 ml比色管中加入一定量的1,8-二羟基蒽醌标准溶液,ph值为3.60的naac-hac缓冲溶液2.0 ml,有机溶剂乙腈2.0 ml,最后定容至10.0 ml;同时做空白对照,在最大激发波长为440 nm,最大发射波长为521 nm处测定一系列溶液的荧光强度。

CoCl2 (a chemical hypoxia-mimetic agent) was used to establish the chemical hypoxia-induced PC12 cell injuries model. Sodium hydrosulfide was used as a H2S donor. The viability of PC12 cells was measured by CCK-8 assay. The percentage of apoptotic cells was assessed by propidium iodide stain flow cytometry. The morphological change of apoptotic cells was tested by using the chromatin dye Hoechst 33258. The mitochondrial membrane potential was analyzed by rhodamine 123 staining and photofluorography. The level of reactive oxygen species in PC12 cells was measured by DCFH-DA staining and photofluorography.

应用化学性缺氧模拟剂CoCl2在PC12细胞建立化学性缺氧损伤模型;以硫氢化钠作为H2S的供体;应用CCK-8比色法检测细胞存活率;碘化丙啶染色流式细胞技术检测细胞凋亡率;Hoechst33258染色检测细胞凋亡的形态学变化;罗丹明123(Rh123)染色荧光显微镜照相检测细胞线粒体膜电位;2',7'-二氯荧光黄双乙酸盐染色荧光显微镜照相检测细胞内的活性氧水平。

Methods Male SD rats were given drinking water containing manganese chloride (MnCl24H2O 0.5g/L and 5.0g/L respectively) for 60 days. At the end of experiment, all the rats of the manganese-exposed groups and control group were decapitated after anaesthesia with 2% sodium phenobarbitone intraperitoneally, the brains were removed and activities of alanine aminotransferase, aspartate aminotransferase, alkaline phosphate, cholinestarase, monoamine oxidase, superoxide dismutase, nitric oxide synthase, and levels of glutathione, maleic dialdehyde, and proteins were biochemically analyzed.

大鼠经饮水连续染锰(MnCl24H2O 0.5g/L和5.0g/L)60d后,应用荧光光度比色法测定新鲜脑组织匀浆的谷丙转氨酶、谷草转氨酶、碱性磷酸酶、胆碱酯酶、单胺氧化酶、氧化亚氮合酶、超氧化物歧化酶活性和谷胱甘肽、丙二醛、蛋白质含量,并设生理盐水对照组。

Infrared, ultraviolet, and mass spectrometry Gaoxiaoyexiang tests showed that benzene p rosin in neighbouring seats husband alkali metal objects with catalytic, the oxide containing hydroxy generated, ethylene, or a functional group, such as the Food oxide product.

红外、紫外、高效液相色谱和质谱测试表明,松香在邻苯二胺席夫碱金属配合物的催化下,氧化生成了含羟基、环氧基、过氧基等官能团的氧化产物。

The reaction was stirred for the given time (the reaction was followed by TLC) after which the methanol was removed and the residual ionic liquid was extracted with diethyl ether (3 15 mL).

反应搅拌为在给定的时间(反应,其次是薄层色谱法)后,其中甲醇被删除,剩余的离子液体中提取二乙酯基醚。

The next year Musa attacked the combined force of Sulayman and the Byzantine emperor Manuel II in Thrace; but when Musa's Serbians and Bulgarians deserted, Sulayman occupied Edirne.

明年穆萨攻击部队的Sulayman和拜占庭皇帝曼努埃尔二世在色雷斯;但是,当穆萨的塞族和保加利亚空无一人, Sulayman占领埃迪尔内。

So they being sent by the Holy Ghost, went to Seleucia: and from thence they sailed to Cyprus.

二人既被圣神派遣,遂下到色娄基雅,又从那里乘船往塞普勒斯。

So they being sent by the Holy Ghost, went to Seleucia: and from thence they sailed to Cyprus.

二人既被圣神派遣,遂下到色娄基雅,又从那里乘船往塞浦路斯。

So, being sent out by the Holy Spirit, they went down to Seleucia

二人既被圣神派遣,遂下到色娄基雅,又从那里乘船往塞浦路斯。

2 So they, sent forth by the holy Spirit, went down to Seleucia and from there sailed to Cyprus.

二人既被圣神派遣,遂下到色娄基雅,又从那里乘船往塞浦路斯。

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Finally, according to market conditions and market products this article paper analyzes the trends in the development of camera technology, and designs a color night vision camera.

最后根据市场情况和市面上产品的情况分析了摄像机技术的发展趋势,并设计了一款彩色夜视摄像机。

Only person height weeds and the fierce looks stone idles were there.

只有半人深的荒草和龇牙咧嘴的神像。

This dramatic range, steeper than the Himalayas, is the upturned rim of the eastern edge of Tibet, a plateau that has risen to 5 km in response to the slow but un stoppable collision of India with Asia that began about 55 million years ago and which continues unabated today.

这一引人注目的地域范围,比喜马拉雅山更加陡峭,是处于西藏东部边缘的朝上翻的边框地带。响应启始于约5500万年前的、缓慢的但却不可阻挡的印度与亚洲地壳板块碰撞,高原已上升至五千米,这种碰撞持续至今,毫无衰退。