二肽

In all, A.subjunquillea.S.lmai methyl alcohol crude extract were research by high pressure liquidchromatography,.separation and purification obtained to 9 kind of compounds, identified 6 kind ofcompounds. The compounds 1～6:β-Amanitin、α-Amanitin、phallisacin、phallisin、phallacidin and phalloidin.

总之，黄盖鹅膏甲醇粗提物应用高效液相分离纯化9种单体化合物，鉴定6种分别为β-鹅膏毒肽、α-鹅膏毒肽、羧基三羟鬼笔毒肽、三羟鬼笔毒肽、羧基二羟鬼笔毒肽和二羟鬼笔毒肽。

All resonance peaks of gramicidin S were assigned.And then,the NMR assignments were used to determine the three dimensional structure of gramicidin S in DPPC reversed micelle.

用二维相关谱和二维NOE谱对反向脂囊泡中短杆菌肽S的共振峰进行了识别，短杆菌肽S所有的共振峰都被指定。

Methods Tissue culture was used to study the cataractous lenses of rats induced by sodium selenite or galactose. At the early stage, the content of nonprotein sulfhydryl, protein sulfhydryl, non-soluble disulfide bond, malonaldehyde and the activities of glutathione peroxidase, glutathione reductase and glutathione-S-transferase were measured in the cataractous lenses, and they were compared with that of the normal lenses. Results The two kinds of material could all induce cataract in rats, sodium selenite being more potent.

采用器官培养方法，以不同浓度亚硒酸钠及半乳糖作用于晶状体，诱发大鼠晶状体形成白内障，在培养初期测定晶状体内非蛋白质巯基（nonprotein sulfhydryl， NP-SH、蛋白质巯基（protein sulfhydryl ，P-SH）和不溶性蛋白质二硫键的含量，脂类过氧化水平，以及与谷胱甘肽代谢有关的谷胱甘肽过氧化物酶(GSH peroxidase， GSH-PX)、谷胱甘肽还原酶(GSH reductase， GSSG-R)及谷胱甘肽硫转移酶(GSH S transferase， GSH-S)的活性。

Since several dipeptides and tripeptides have been reported to be played the important roles in biophysiological functions, specific peptidyl-peptiases may have great potentialities for use in food biotechnology industry.

由於某些二胜肽及三胜肽被研究报告为具有许多生理功能之活性胜肽，因此，具有将蛋白质水解成特殊小胜肽之分解酶的研究与开发，在食品生物技术领域上具有很大的应用潜力。

To synthesize the octreotide, Phe-Leu-Glu-Glu-Leu and C_(15)H_(31)-CO-Lys-Thr -Thr-Lys-Ser peptides, the strategies are as followings: Fmoc- group as protective group for α-NH_2; TBTU, DIEA and HOBt as coupling reagent; and ethanedithiol -phenol - thioanisole - H_2O - TFA ( 2.5:5:5:5:82.5 , V/V ) as cleaving solution.

采用Fmoc固相合成法，以苯并三哗四甲基脲四氟硼酸盐、二异丙基乙胺和1-羟基苯骈三氮唑为缩合剂；苯酚—乙二硫醇—苯甲硫醚—水—三氟乙酸(5：5：2.5：5：82.5，V/V)为切割试剂，合成了奥曲肽、Phe-Leu-Glu-Glu-Leu及C_(15)H_(31)-CO-Lys-Thr-Thr-Lys-Ser三种小分子多肽，并对奥曲肽的合成规模进行了放大。

Furthermore, influences of different phosphate salts at various concentrations on the ionization efficiency of phosphopeptide were investigated systematically, finding that the signal intensity for phosphopeptide 48FQEEQQQTEDELQDK63 digested from β-casein were 5 to 8 times increased in an optimized condition with 10 mmol/L ammonium monobasic phosphate or 3 to 4 times increased with 10 mmol/L ammonium dibasic phosphate as additive to matrix 2,5-dihydroxybenzoic acid, respectively.

考察了两种适合于磷酸化肽离子化的基质类型2，5-二羟基苯甲酸和2，4，6-三羟基苯乙酮。用2，5-二羟基苯甲酸作为基质时，当加入10 mM 磷酸氢二铵时，磷酸化蛋白质β-casein的磷酸肽 48FQEEQQQTEDELQDK63的离子化效率可以增强5-8倍，当加入10 mM磷酸二氢胺时，磷酸肽的离子化效率可以增强3-4倍。

The content of essential amino acid and flavor amino acid of experiment groups were higher than control group, especially group 1.0% small peptide were increased by 6.4% and 2.5%; IMP content of experiment groups was increased in different extent, especially group 1.0% small peptide was 0.17mg/100mg and increased by 70.0%(P.05); The content of palmitoleic acid、 octadeca、 arachidonic、 arachidic and stearic acid of experiment groups were higher than control group indifferent extent.

随着小肽用量的增加，成鲤肌肉中的总氨基酸含量呈上升趋势，必须氨基酸和风味氨基酸的含量也随之提高，尤其是1.0％小肽组的必须氨基酸和风味氨基酸的含量分别比对照组提高6.4％和2.5％，其中谷氨酸的含量最高，为10.70mg／100mg，比对照组提高14.0％(P＜0.05)；随着饵料中小肽用量的增加，成鲤肌肉的肌苷酸含量显著提高(P＜0.05)，1.0％小肽组的IMP含量高达0.17mg／mg，比对照组提高70.0％；成鲤肌肉中的油酸和二十？

This study is design and preparation of active peptide analogues of mung bean trypsin inhibitor Lys fragment, Trichosanthes trypsin inhibitor I, carcinoembryonic antigen peptide and aspartame using solid and liquid phase peptide synthesis methods.

本论文研究活性多肽类似物的设计、制备和功能，分为四个部分，采用多肽固相和液相合成的方法，分别合成了活性多肽绿豆胰蛋白酶抑制剂 Lys片段长链16肽、天花粉胰蛋白酶抑制剂、癌胚抗原肽、甜味肽等一系列类似物，并测定了这些类似物的生物活性，有以下新的研究结果：1、furin酶抑制剂的设计、制备和功能；2、芳香氨基酸芳环之间的π-π共轭能部分补偿二硫键的作用；3、癌胚抗原肽Gly4被L-Pro替代可增加与HLA-A2分子的结合；4、肽键对甜味肽保持甜味是必须的。

Highly active, small-molecule furin inhibitors are attractive drug candidates to fend off bacterial exotoxins and viral infection. Based on the 22-residue, active Lys fragment of the mung bean trypsin inhibitor, a series of furin inhibitors were designed and synthesized, and their inhibitory activity toward furin and kexin was evaluated using enzyme kinetic analysis. The most potent inhibitor, containing 16 amino acid residues with a Ki value of 2.45×10~(-9) M for furin and of 5.60×10~(-7) M for kexin, was designed by three incremental approaches.

本论文研究活性多肽类似物的设计、制备和功能，分为四个部分，采用多肽固相和液相合成的方法，分别合成了活性多肽绿豆胰蛋白酶抑制剂Lys片段长链16肽、天花粉胰蛋白酶抑制剂、癌胚抗原肽、甜味肽等一系列类似物，并测定了这些类似物的生物活性，有以下新的研究结果：1、furin酶抑制剂的设计、制备和功能；2、芳香氨基酸芳环之间的π-π共轭能部分补偿二硫键的作用；3、癌胚抗原肽Gly4被L-Pro替代可增加与HLA-A2分子的结合；4、肽键对甜味肽保持甜味是必须的。

By using lysozyme, RNase A and protein disulfide isomerase as model proteins, we present a hypothesis that protein cross-linking can be accomplished in three concerted steps:(1) a change in protein conformation;(2) formation of interchain disulfide bonds; and (3) formation of interchain isopeptide cross-links.

我们以溶菌酶、核糖核酸酶A和蛋白质二硫键异构酶为模型对异肽键进行了体外详细的研究，提出了解释异肽键形成机理的假说：1蛋白质发生构象变化包括二级结构的改变；2蛋白质形成分子间二硫键：3二硫键的形成帮助蛋白质形成了分子间异肽键。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。