二聚的

To study the expression, purification and bioactivity of human augmenter of liver regeneration in Pichia Pastoris, the expression plasmid pPICZαA- ALR was constructed and transformed into P. Pastoris by the method of electroporation transformation. Induced with 0.5% methanol, the 30 kD protein in the culture supernatant of recombinant P. Pastoris was confirmed to be rhALR by SDS-PAGE and Western blot. Quantitative analysis showed that the target protein was in a level of 66% of the total protein of the culture supernatant, with a yield of 40mg/L.we had performed DEAE anion exchange chromatography two times with excessive and regular adsorption quantity consecutively, and then the rhALR above 95% purity and 52% protein recovery could be obtained by G75 molecular sieve chromatography at last step. The bioactivity assay of the purified product showed that rhALR could stimulate the proliferation of HepG2, SMMC-7721 and NIH-3T3 in vitro.

为在毕赤酵母中分泌表达人肝再生增强因子，以色谱法分离纯化后进行体外活性研究，构建表达载体pPICZαA- ALR，经电穿孔转入毕赤酵母中，用0.5％甲醇诱导表达；重组酵母培养上清经SDS-PAGE电泳和western blot鉴定后表明， rhALR以分子量为30kD的二聚体为主；定量分析结果表明，重组酵母培养上清中rhALR约占总蛋白的66％，表达量约为40mg/L；经DEAE柱和G75柱纯化后，获得的rhALR纯度大于95％，得率为52％；体外生物学活性实验表明，rhALR能明显促进HepG2、SMMC-7721和NIH-3T3细胞的增殖。

Dimerization behavior of NiTSPc molecules was characterized by electronic absorption spectra, and changes in the absorption spectra were correlated with various stages of the sol-gel process.

对NiT SPc在此过程中电子吸收光谱的变化进行了系统的跟踪研究以考察其在溶胶凝胶体系中的二聚行为，并结合复合体系微化学环境的改变对其进行了自洽解释。

It indicates that the formation of NO dimer is favorable to the NO direct decomposition on the copper surface. The Mülliken population analysis shows that the average charge on Cu is 0.66 e, when the singlet state dimer interacts with the copper.

同时电荷布居分析表明，单重态的二聚体与铜作用时，铜原子上的平均电荷达到0.66e，说明在这种吸附形式中铜被离子化的倾向较大，而且这种吸附形式最有利於NO的分解。

Moreover, we have found the pentapeptide RGSGR, which is present at the C terminus of PatS and blocks heterocyst formation, inhibits the DNA-binding of HetR dimer and prevents hetR up-regulation.

另外，位于PatS蛋白C端的五肽RGSGR可以通过抑制HetR同源二聚体的DNA结合活性，阻断hetR的上调表达而影响异型胞的形成过程。

The industrial manufacturing technique, the trend of development of neohexene ,and the characteristics of these production technique were reviewed and analyzed in the paper, particularlyabout the selective dimerization of isobutylene and metathesis of βdiisobutylene with ethylene were introduced.

对新己烯生产技术的现状和今后的发展趋势进行了综述，分析了由异丁烯和乙烯生产新己烯的技术特征，讨论了烯烃二聚工艺和催化剂、烯烃复分解工艺及相应的催化剂，指出了各种工艺的优势和不足。

After subjected to the streaming treatment, the dispersion of the loaded Mo species pronouncedly increased, and some Mo species with small particle size migrated into the zeolite channels and reacted with the Brnsted acid sites to form (Mo2O5)(superscript 2+) dimers.

经高温蒸气处理后，催化剂上Mo物种的分散度显著提高，有较多的Mo物种迁移至分子筛的孔道内，并与B酸发生强相互作用生成钼氧二聚体，有效地增强了催化剂的抗积炭能力。

The effects of PEG addition to the precursor solution on the microstructure of the resultant thin films were studied.

涂层的形貌，如孔的大小和孔的分布可以通过聚乙二醇的加入量和分子量来控制。

The obtained activation barrier of β-scission of 1-butene is 217 kJmol^(-1) in the reverse reaction of ethene dimerization.

反应的逆过程也就是1-丁烯在酸性分子筛表面的1-丁基烷氧化合物发生B分裂反应，计算所得相应的l-丁烯B分裂反应的能垒为217kJmol^(-1)，远高于相应的乙烯二聚反应能垒。

Conformation of CaM has changed greatly after bound by Ci, with the result that the binding sites and interaction module can be roughly determined after chemical shifts assignment, perturbation and calculation of KD, which supports the supposal that CaM and CNA C-terminal were in symmetrical homodimer complex2. In addition, the possible position of"NewCBD"in the scale of residue 430-450 and classification of functional domains in C-terminal have also been discussed.

结合了Ci的CaM构象较之于其自由状态发生很大改变，经化学位移指认、微扰实验和解离平衡常数计算后可以初步确定两者的结合位点以及作用模式，支持了CaM与CNA C末端片段以对称的同二聚体形式命名为(CaM·Ci2复合的猜想，推断出"NewCBD"可能存在于430-450区间，对现有的C末端功能区划分作出了一些改进。

Yang E,Korsmeyer SJ.Molecular thanatopsis:a discourse on the BCL-2 family and cell death.Blood,1996,88:386[3] Campos L,Rouanlt JP,Salido O,et al.High expression of BCL-2 protein in acute myeloid leukemia cells is associated with poor response to chemotherapy.

BCL-2是新一类癌基因——凋亡调控基因BCL-2家族的代表，其编码26kd的蛋白存在于线粒体、核膜及内质网，与同家族的其它成员组成不同比例的同、异二聚体而影响凋亡启动[6]。

We have no common name for a mime of Sophron or Xenarchus and a Socratic Conversation; and we should still be without one even if the imitation in the two instances were in trimeters or elegiacs or some other kind of verse--though it is the way with people to tack on 'poet' to the name of a metre, and talk of elegiac-poets and epic-poets, thinking that they call them poets not by reason of the imitative nature of their work, but indiscriminately by reason of the metre they write in.

索夫农 、森那库斯和苏格拉底式的对话采用的模仿没有一个公共的名称；三音步诗、挽歌体或其他类型的诗的模仿也没有——人们把&诗人&这一名词和格律名称结合到一起，称之为挽歌体诗人或者史诗诗人，他们被称为诗人，似乎只是因为遵守格律写作，而非他们作品的模仿本质。

The relationship between communicative competence and grammar teaching should be that of the ends and the means.

交际能力和语法的关系应该是目标与途径的关系。