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On the basis of the photoreactivity, spectroscopy, and spectrometry data, we determined the structure of the new photoproduct to be a nonadjacent cis-anti cyclobutane dimer formed between T2 and T7 with the absolute stereochemistry depicted by structure I (Figure 8B).

结论根据photoreactivity、分光学和光谱数据,我们确定新的photoproduct的结构是nonadjacent同边反cyclobutane二聚体被形成在T2和T7之间以结构描述的绝对立体化学I (图8B)。

Keywords: DNA photoreactivation; CPD photolyase; tryptophan; electron transfer; cyclobutane pyrimidine dimer

中文关键词: DNA光复活;CPD光解酶;色氨酸;电子转移;嘧啶二聚体

Objective To observe the production and removal of photo-product, cyclobutane pyrimidine dimers, in HaCaT cells induced by UVB irradiation and intervention effect of ligustrazine, and to investigate its photo-protective mechanisms on HaCaT cells damaged from UVB irradiation.

目的观察中波紫外线辐射后永生化角质形成细胞株中光产物环丁烷嘧啶二聚体的产生和清除情况,观察川芎嗪对UVB辐射后细胞光产物的影响并探讨其保护作用机制。

UV-B radiation during the cell cycle induced accumulation of cyclobutane pyrimidine dimers.

单细胞凝胶电泳检测结果表明,UV-B辐射引起拟南芥根尖细胞内积累大量的环丁烷嘧啶二聚体

Ozone College depletion in stratosphere has led to the increase of solar UV-B radiation reaching to the earth surface, which would damage DNA of plant epidermal cells and produce cyclobutane pyrimidine dimmers (CPD and 6-4 photoproducts.

臭氧衰减导致地表紫外辐射增强,而紫外线辐射对植物表皮细胞的DNA具有损害作用,可导致环丁烷嘧啶二聚体和6,4-光产物的形成。

Aim To investigate the production and removal of cyclobutane pyrimidine diners after UVB irradiation and the effect of baikal skullcap root, szechwan lovge rhizome and epigallocatechingallate on the irradiation damage in HaCaT cells, and finally, to clarify the probable regulatory mechanisms. Methods HaCaT cells were cultured in RMPI-1640 medium with 10% fetal bovine serum. The time and dosage of UVB irradiation were conducted according to the experimental design. Tested medicines were added into the medium before or after UVB irradiation.

目的 观察中波紫外线辐射后HaCaT细胞光产物胸腺嘧啶二聚体(cyclobutanepyrimidine dimers, CPDs)的生成和清除情况及3种中药活性成分:黄芩(baikal skullcap root, BSR)、川芎(szechwan lovge rhizome, SLR)和表没食子儿茶素没食子酸酯[-epigallocatechin-3-gallate, EGCG]对该过程的干预情况并探讨上述药物对相关调控分子的作用机制。

Our experiment results suggest that the dimeric form of the enzyme is the major form for biological activity.

根据这一实验结果我们提出:二聚体是冠状病毒3CL蛋白酶的主要生物活性形式。

By co-immunoprecipitation, a complex of tau with Hsc70 and dimeric CHIP was observed.

免疫共沉淀发现tau蛋白、Hsc70和CHIP二聚体共存。

Mass spectrometry provided evidence for the dimeric serotonin species from UV radiation studies.

以质谱分析照射 UV 后变色的 serotonin 标准品,可侦测到 serotonin 形成二聚体的结构。

C. hytivus exhibited the typical hybrid dimeric banding pattern. The difference in band intensity between C.

新种的AAT酶谱在两个多态性位点表现了典型的杂种二聚体谱型。

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