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The research presented here is aimed at exploring the effect of DTT on the in vitro fibril formation of hen egg-white lysozymes with four disulfide bonds. In this work, we specifically addressed the effect of DTT concentration under different incubation temperatures on the hen lysozyme structure and fibril formation, the effect of addition of DTT at later time points on the amyloid fibrillization of hen lysozymes, and the effect of concentration ratio between reduced and oxidized forms of DTT on the fibril formation of lysozymes.

本研究以具有四对双硫键的母鸡蛋白溶菌酶为蛋白质模式系统,在含盐类之酸性溶液环境下诱导生成类淀粉纤维,再配合二硫代苏糖醇破坏溶菌酶中的双硫键,探讨:(1)不同温度下加入不同DTT浓度对溶菌酶类淀粉纤维形成之影响,(2)不同生长时期加入DTT对溶菌酶类淀粉纤维形成之影响,(3)不同比例之DTT和氧化态DTT浓度对溶菌酶类淀粉纤维形成之影响。

METHODS: The bile was treated with 3 different methods, including the 2D-Clean-up kit group: the bile sample was dissolved with lysis buffer (contains: 7 mol/L carbamide, 2 mol/L thiourea, ammonium persulfate with mass concentration of 0.4 g/L, 65 mmol/L dithiothreitol, carrier ampholytes with mass concentration of 0.2 g/L).

将标本分别按3种不同方法进行处理:①2D-Clean-up试剂盒处理胆汁标本:用裂解液,7 mol/L尿素,2 mol/L硫脲,质量浓度为0.4 g/L的过硫酸铵,65 mmol/L二硫苏糖醇,质量浓度为0.2 g/L的载体两性电解质溶解沉淀过夜。

Compared to wild-type plants, the onset of flowering was advanced. The results of this study may be useful in the mulberry gene engineering and the results may be helpful to study the regulation of SBPase.Rubisco activase is a soluble chloroplast protein, coded in nucleus, and has the activation of Rubisco in photosynthetic autotrophs.

本研究为桑树基因工程提供了有效的候选基因,为深入研究SBPase的分子调控机制奠定了基础。1,5-二磷酸核酮糖羧化酶活化酶广泛存在于光合生物中,它是一种由核基因编码的叶绿体蛋白,具有调节Rubisco活性的功能。

Simulated water stress experiment on the five-leaf stage of maize growth with different concentrations of polyethylene glycol in nutrient solution was performed to study the effects of water stress and rewatering on chlorophyll content,chlorophyll fluorescence kinetic parameters,photosynthetic parameters and soluble sugar content.

采用聚乙二醇模拟干旱胁迫方法,研究玉米五叶期水分胁迫期间及复水后叶片叶绿素含量、叶绿素荧光动力学参数、光合参数和可溶性糖的补偿效应。

Simultaneously, the activity and coding gene expression of ribulose 1, 5-bisphosphate carboxylase, phosphoenolpyruvate carboxylase, and glycollic oxidase all decrease in two rice accessions under low potassium supply while significant decrease in non-allelopathic rice "Lemont".

水稻光合作用相关酶的分析结果表明,低钾条件下核酮糖-1,5-二磷酸羧化酶、磷酸烯醇式丙酮酸羧化酶和乙醇酸氧化酶的活性及其基因表达强度均降低,但化感水稻&PI312777&的下降幅度显著小于非化感水稻&Lemont&。

Moreover, MeJA induced defense genes, such as pathogenesis-related proteins (PR2a), phenylalanine ammonia-lyase, BX9, and TPS. MeJA also increased DIMBOA and Vannillic acid content of leaves and roots significantly but leaf p-Hydroxybenzoic acid concentration was decreased significantly and Protocatechuric acid was produced in roots only.

1MeJA叶片处理能诱导叶片和根系中丙二烯氧化物环化酶基因、糖基转移酶(BX9)基因、苯丙氨酸转氨酶基因的表达,使叶片和根系中丁布含量升高,同时,6μL/L MeJA处理使第二叶中原儿茶酸含量显著降低,诱导根中产生原儿茶酸,使第二叶中香草酸含量和根中未知物质峰面积显著增大。

I want 12 colors and lollipops on the ceiling.

我想要十二种颜色的天花板上面还有很多的棒棒糖。

The preferred cytokine release inhibiting compounds are dimeric gallotannins having a linker molecule that misaligns the carbohydrate cores of the compounds.

优选的细胞因子释放抑制化合物是具有连接分子的二聚单宁,所述连接分子误调该化合物的糖核。

In this dissertation the method of orthogonal test design was applied to discuss the relationship between the extrusion system parameters and the filtration time and some other important indexes of worts.

本文采用四因素五水平二次正交旋转组合试验设计,探讨了挤压系统参数与糖化醪过滤时间及麦汁主要指标之间的关系。

Effects of soil treatments with different pH values on aboveground growth, above-ground biomass, and contents of malonaldehyde and soluble sugar were discussed by randomized block design. Result showed that the whithered tips of A.

试验采用随机区组设计,观察研究耐寒指标及不同pH土壤处理对其地上部分生长、地上部分生物量积累、叶片丙二醛和可溶性糖含量的影响。

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What would he tell Judith and the children?

他将怎样告诉朱迪丝和孩子们呢?

I this is at that time, the opinion with peacockish true girl is full of in a heart.

这就是当时的我,一个心中布满虚荣的女孩子真实的想法。

Oh, and I bought myself a new laptop," he said."

哦,我还给我自己买了一个新笔记本"他说。"