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Our data are showed that apoptosis parameters including DNA fragmentation, nuclear morphological change and sub-G1 fraction, appeared after 24 h by 5 μM rottlerin treatment. Furthermore, rottlerin caused a decrease in 72% of ODC protein expression and an increase in threonine, rather than serine and tyrosine dephosphorylation on ODC enzyme compared with the control after incubation for 10 h. To determine whether ODC could down-regulate rottlerin-induced apoptosis, HL60 cells were stably transfected with ODC cDNA. HL60-ODC cells exhibited a 2.5-fold increase in ODC protein compared with vector only (HL60-pcDNA3) or HL60 cells.

在我们筛选天然化合物实验中,发现rottlerin抑制鸟胺酸去羧化活性最具成效,之前的研究人员已知蛋白激Cd调节鸟胺酸去羧化的活性,因此在人类白血病HL60细胞处理rottlerin的实验中发现加入rottlerin二十四小时后,细胞进行计划性死亡,并可侦测到DNA片段、细胞核浓缩及sub-G1提增;而细胞处理药物十小时后与控制组细胞比较,观察到鸟胺酸去羧化的蛋白表现与活性下降50﹪以上;除此以外,测定鸟胺酸去羧化去磷酸化的情况,发现於酥胺酸位置有显著差异,而丝胺酸和酪胺酸位置并无差异。

The results showed that intermidiate 1, 2-propanediol-sebacate of low relative molecular mass was synthesized with toluene-p-sulfoinc acid as catalyst, benzene as watercarrying agent, and of mole ratio of sebacic acid to 1, 2-propanediol of 1:4. The glass-transition temperatures of PPSC were below 0 ℃ which confirmed that PPSC would be in a high-elastic state of ambient temperature and human body temperature. PPSC was a elastomer with moderate crosslinkage, low module and high elongation, and had rapid degradation ratio (degradation degree more than 50%) in phosphate (pH value 7.4) at 37℃.

结果表明,以对甲苯磺酸为催化剂、苯为带水剂,在酸醇比为1,4的条件下可合成较低相对分子质量的中间体癸二酸聚酯二元醇;PPSC弹性体的玻璃化转变温度在0℃以下,于室温及人体温度下处于高弹态;PPSC为适度交联的、低模量高伸长的弹性体,其在37℃、pH值为7.4的磷酸盆缓冲溶液中具有较快的降解性(降解度超过50%)。

Diammonium phosphate,NKP compound fertilizer,granular superphosphate,titanium dioxide,sodium silicofluoride,potassium silicofluoride,phenyl Peri acid,Peri acid,Laurent's acid,antioxidant,pyrite.

提供产品:磷酸二铵、NPK复合肥、造粒磷肥、钛白粉、氟硅酸钠、氟硅酸钾、苯基周位酸、周位酸、劳伦酸、橡胶防老剂、硫铁矿。

The result showed that application of diammonium phosphate coated with palygorskite at 225kghm^-(2), the effective siliquae and yield of rape increased by 2.27% and 255.33% respectively compared with control.

结果表明:施用坡缕石包衣的磷酸二铵225kghm^(-2)后,油菜有效结角数和经济产量分别较对照提高了2.27%和255.33%;油菜后复种小麦,施用坡缕石包衣肥300kghm^(-2)后,小麦生物产量比对照增加了57.67%。

The effect of characteristics, including concentration and flow velocity of absorbent and feeding gas, on the CO_2 absorption process has been widely investigated. And the active materials of different concentration, including Potassium phosphate tribasic、 Potassium tetraborate、 N-methyldiethanolamine、 2-Amido-2-methyl-l-propylacohol and Piperazine, were added in the absorbent, of which the effect has also been investigated.

主要考察了气液流速及其浓度等操作参数对CO_2吸收性能的影响;同时研究了在氨基酸盐吸收剂中添加各种活化剂,包括磷酸钾、硼酸钾、N-甲基二乙醇胺、2-氨基-2-甲基-1-丙醇和哌嗪对CO_2分离效果的影响;并对膜组件运行的稳定性进行了初步考察。

The results from the model are in good agreement with the experimental data. Potassium phosphate tribasic、 Potassium tetraborate、 and Piperazine are of obvious active affection for enhancement foctor, where N-methyldiethanolamine and 2-Amido-2-methyl-l-propylacohol are negative ones in all additives.

在反应增强因子的研究方面,其模型计算值与实验值符合较好;复合吸收剂体系中,活化剂N-甲基二乙醇胺和2-氨基-2-甲基-1-丙醇对增强因子起了消极影响,活化剂磷酸钾、硼酸钾和哌嗪对增强因子则具有明显的促进作用。

The results from the model are in good agreement with the experimental data. Potassium phosphate tribasic、Potassium tetraborate、and Piperazine are of obvious active affection for enhancement foctor, where N-methyldiethanolamine and 2-Amido-2-methyl-1-propylacohol are negative ones in all additives.

在反应增强因子的研究方面,其模型计算值与实验值符合较好;复合吸收剂体系中,活化剂N-甲基二乙醇胺和2-氨基-2-甲基-1-丙醇对增强因子起了消极影响,活化剂磷酸钾、硼酸钾和哌嗪对增强因子则具有明显的促进作用。

The results from the model are in good agreement with the experimental data. Potassium phosphate tribasic、Potassium tetraborate、and Piperazine are of obvious active affection for enhancement foctor, where N-methyldiethanolamine and 2-Amido-2-methyl-1-propylacohol are negative ones in all additives.

在反应增强因子的研究方面,其模型计算值与实验值符合较好;复合吸收剂体系中,活化剂N-甲基二乙醇胺和2-氨基-2-甲基-1-丙醇对增强因子起了消极影响,活化剂磷酸钾、硼酸钾和对增强因子则具有明显的促进作用。

Twelve new Schiff bases of 5-amino-1H-1,2,3,4-tetrazolyl were synthesized. These Schiff bases were further converted into α-(5- tetrazolyl)-aminoalkyl phosphonates.

合成了12个新的5—氨基—1H—1,2,3,4—四唑的Schiff碱,而后与亚磷酸二乙酯反应,合成出了12个新的含四唑基的α-氨基烃基膦酸酯,通过IR,^1H NMR,^31P NMR和元素分析证实了它们的结构。

Methods: Eleven B-CLL patients were studied. Leukemic lymphocytes with (n=8) or without (n=3) P2z receptors were exposed in vitro to ATP, benzoylbenzoic-ATP, 2-methylthio-ATP(2MeSATP), adenosine-5′-[γ-thio] triphosphate, and other nucleosides for 8h. Apoptosis was detected by electron microscopy, agarose gel electrophoresis, and quantitative TdT assay. Results:Apoptosis was detected only in leukemic lymphocytes with P2z receptors. By using the quantitative assay, ATP-inducing DNA strand breaks were found to occur specifically for BzATP, ATP and 2MeSATP, but not for ATP-γS and other nucleosides.

将表达P2z受体[P2z]与不含P2z受体[P2z]的两组CLL细胞分别同1.0mmol/L三磷酸腺苷体外培养8小时,以电镜、DNA凝胶电泳和定量DNA 3′端TdT法检测细胞凋亡;并对ATP、苯甲酰苯甲酸ATP、2-甲基硫ATP(2MeSATP)、γ-硫代ATP及其它核苷的诱导效应和氧化型ATP、1-[N,O-二(5-异喹啉碘酰基)N-甲基-L-酪氨酰]-4-苯哌嗪KN-62)的抑制效应做定量研究。

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According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm~150rpm时取得最大值,此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世,形体几无变化。

When I was in school, the rabbi explained everythingin the Bible two different ways.

当我上学的时候,老师解释《圣经》用两种不同的方法。